Suppression of the NF-κB signalling pathway by ergolide, sesquiterpene lactone, in HeLa cells

Journal of Pharmacy and Pharmacology

Volumn 59, Issue 4, 2007, Pages 561-566

  Jae, Kwang Chun ^a   Seo, Dong Wan ^b   Seong, Hoon Ahn ^c   Jae, Hyun Park ^a   You, Jueng Soo ^a   Lee, Chang Hee ^a   Jae, Cheol Lee ^a   Yong, Kee Kim ^d   Han, Jeung Whan ^a  

^a Sungkyunkwan University   (South Korea)

^b Kangwon National University   (South Korea)

^c Hanyang University   (South Korea)

^d Catholic Kwandong University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLOOXYGENASE 2; DNA; ERGOLIDE; I KAPPA B; I KAPPA B KINASE; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INDUCIBLE NITRIC OXIDE SYNTHASE; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; MITOGEN ACTIVATED PROTEIN KINASE P38; PHORBOL 13 ACETATE 12 MYRISTATE; PROTEIN KINASE C ALPHA; SESQUITERPENE LACTONE; SYNAPTOTAGMIN I; UNCLASSIFIED DRUG; LACTONE; SESQUITERPENE; TRANSCRIPTION FACTOR RELA;

ARTICLE; CONTROLLED STUDY; DNA BINDING; DRUG MECHANISM; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME REGULATION; GENE EXPRESSION; GENE TRANSLOCATION; GENETIC TRANSCRIPTION; HELA CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; PROTEIN DEGRADATION; PROTEIN PHOSPHORYLATION; CHEMISTRY; DRUG ANTAGONISM; DRUG EFFECT; INULA; METABOLISM; PHOSPHORYLATION; REPORTER GENE; SIGNAL TRANSDUCTION;

DNA; GENE EXPRESSION; GENES, REPORTER; HELA CELLS; HUMANS; I-KAPPA B KINASE; I-KAPPA B PROTEINS; INULA; LACTONES; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE 3; NF-KAPPA B; P38 MITOGEN-ACTIVATED PROTEIN KINASES; PHOSPHORYLATION; PROTEIN KINASE C-ALPHA; SESQUITERPENES; SIGNAL TRANSDUCTION; TETRADECANOYLPHORBOL ACETATE; TRANSCRIPTION FACTOR RELA; TRANSCRIPTION, GENETIC;

EID: 34250155644     PISSN: 00223573     EISSN: None     Source Type: Journal    
DOI: 10.1211/jpp.59.4.0011     Document Type: Article

References (29)

1. Chen, C. C., Wang, J. K. (1999) p38 but not p44/42 mitogen-activated protein kinase is required for nitric oxide synthase induction mediated by lipopolysaccharide in RAW 264.7 macrophages. Mol. Pharmacol. 55: 481-488
2. Chen, C., Chen, Y. H., Lin, W. W. (1999) Involvement of p38 mitogen-activated protein kinase in lipopolysaccharide-induced iNOS and COX-2 expression in J774 macrophages. Immunology 97: 124-129
3. Chen, L., Fischle, W., Verdin, E., Greene, W. C. (2001) Duration of nuclear NF-κB action regulated by reversible acetylation. Science 293: 1653-1657
4. Delhase, M., Hayakawa, M., Chen, Y., Karin, M. (1999) Positive and negative regulation of IκB kinase activity through IKKβ subunit phosphorylation. Science 284: 309-313
5. Garcia-Pineres, A. J., Lindenmeyer, M. T., Merfort, I. (2004) Role of cysteine residues of p65/NF-κB on the inhibition by the sesquiterpene lactone parthenolide and N-ethyl maleimide, and on its trans-activating potential. Life Sci. 75: 841-856
6. Genot, E. M., Parker, P. J., Cantrell, D. A. (1995) Analysis of the role of protein kinase C-α, -ε and -ζ in T cell activation. J. Biol. Chem. 270: 9833-9839
7. Hatada, E. N., Krappmann, D., Scheiderit, C. (2000) NF-κB and the innate immune response. Curr. Opin. Immunol. 12: 52-58
8. Hehner, S. P., Heinrich, M., Bork, P. M., Vogt, M., Ratter, F., Lehmann, V., Schulze-Osthoff, K., Dröge, W., Schmitz, M. L. (1998) Sesquiterpene lactones specifically inhibit activation of NF-κB by preventing the degradation of IκB-α and IκB-β. J. Biol. Chem. 273: 1288-1297
9. Je, J. H., Lee, J. Y., Jung, K. J., Sung, B., Go, E. K., Yu, B. P., Chung, H. Y. (2004) NF-κB activation mechanism of 4-hydrocyhexenal via NIK/IKK and p38 MAPK pathway. FEBS Lett. 566: 183-189
10. Karin, M. (1999) How NF-κB is activated: the role of the IκB kinase (IKK) complex. Oncogene 18: 6867-6874
11. Karin, M., Ben-Neriah, Y. (2000) Phosphorylation meets ubiquitination: the control of NF-κB activity. Annu. Rev. Immunol. 18: 621-663
12. Karin, M., Delhase, M. (2000) The IκB kinase (IKK) and NF-κB: key elements of proinflammatory signaling. Semin. Immunol. 12: 85-98
13. Kumar, S., Rabson, A. B., Gelinas, C. (1992) The RxxRxRxxC motif conserved in all Rel/κB proteins is essential for the DNA-binding activity and redox regulation of the v-Rel oncoprotein. Mol. Cell. Biol. 12: 3094-3106
14. Lahti, A., Lahde, M., Kankaanranta, H., Moilanen, E. (2000) Inhibition of extracellular signal-regulated kinase suppresses endotoxin-induced nitric oxide synthesis in mouse macrophages and in human colon epithelial cells. J. Pharmacol. Exp. Ther. 294: 1188-1194
15. Lozano, J., Berra, E., Municio, M. M., Diaz, Meco, M. T., Dominguez, I., Sanz, L., Moscat, J. (1994) Protein kinase C zeta isoform is critical for κB-dependent promoter activation by sphingomyelinase. J. Biol. Chem. 269: 19200-19202
16. Lyss, G., Schmidt, T. J., Merfort, I., Pahl, H. L. (1997) Helenalin, an anti-inflammatory sesquiterpene lactone from Arnica, selectively inhibits transcription factor NF-κB. Biol. Chem. 378: 951-961
17. Lyss, G., Knorre, A., Schmidt, T. J., Pahl, H. L., Merfort, I. (1998) The anti-inflammatory sesquiterpene lactone helenalin inhibits the transcription factor NF-κB by directly targeting p65. J. Biol. Chem. 273: 33508-33516
18. Matthews, J. R., Wakasugi, N., Virelizier, J. L., Yodoi, J., Hay, R. T. (1992) Thioredoxin regulates the DNA binding activity of NF-κB by reduction of a disulphide bond involving cysteine 62. Nucleic Acids Res. 20: 3821-3830
19. Mazor, R. L., Menendez, I. Y., Ryan, M. A., Fiedler, M. A., Wong, H. R. (2000) Sesquiterpene lactones are potent inhibitors of interleukin 8 gene expression in cultured human respiratory epithelium. Cytokine 12: 239-245
20. Nick, J. A., Avdi, N. J., Young, S. K., Lehman, L. A., McDonald, P. P., Frasch, S. C., Billstrom, M. A., Henson, P. M., Johnson, G. L., Worthen, G. S. (1999) Selective activation and functional significance of p38α mitogen-activated protein kinase in lipopolysaccharide-stimulated neutrophils. J. Clin. Invest. 103: 851-858
21. Rungeler, P., Castro, V., Mora, G., Gören, N., Vichnewski, W., Pahl, H. L., Merfort, I., Schmidt, T. J. (1999) Inhibition of transcription factor NF-κB by sesquiterpene lactones: a proposed molecular mechanism of action. Bioorg. Med. Chem. 7: 2343-2352
22. Shin, S. G., Kang, J. K., Lee, K. R., Lee, H. W., Han, J. W., Choi, W. S. (2005) Suppression of inducible nitric oxide synthase and cyclooxygenase-2 expression in RAW 264.7 macrophages by sesquiterpene lactones. J. Toxicol. Environ. Health A 68: 2119-2131
23. Tak, P. P., Firestein, G. S. (2001) NF-κB: a key role in inflammatory diseases. J. Clin. Invest. 107: 7-11
24. Trushin, S. A., Pennington, K. N., Algeciras-Schimnich, A., Paya, C. V. (1999) Protein kinase C and calcineurin synergize to activate IκB kinase and NF-κB in T-lymphocytes. J. Biol. Chem. 274: 22923-22931
25. Vane, J. R., Mitchell, J. A., Appleton, I., Tomlinson, A., Bishop-Bailey, D., Croxtall, J., Willoughby, D. A. (1994) Inducible isoforms of cyclooxygenase and nitric-oxide synthase in inflammation. Proc. Natl Acad. Sci. USA 91: 2046-2050
26. Vertegaal, A. C., Kuiperij, H. B., Yamaoka, S., Courtois, G., van der Eb, A. J., Zantema, A. (2000) Protein kinase C-α is an upstream activator of the IκB kinase complex in the TPA signal transduction pathway to NF-κB in U2OS cells. Cell Signal. 12: 759-768
27. Whan Han, J., Gon Lee, B., Kee Kim, Y., Woo Yoon, J., Kyoung Jin, H., Hong, S., Young Lee, H., Ro Lee, K., Woo Lee, H. (2001) Ergolide, sesquiterpene lactone from Inula britannica, inhibits inducible nitric oxide synthase and cyclo-oxygenase-2 expression in RAW 264.7 macrophages through the inactivation of NF-κB. Br. J. Pharmacol. 133: 503-512
28. Woronicz, J. D., Gao, X., Cao, Z., Rothe, M., Goeddel, D. V. (1997) IκB kinase-β: NF-κB activation and complex formation with IκB kinase-α and NIK. Science 278: 866-869
29. Yoon, J. W., Kang, J. K., Lee, K. R., Lee, H. W., Han, J. W., Seo, D. W., Kim, Y. K. (2005) β-Carboline alkaloid suppresses NF-κB transcriptional activity through inhibition of IKK signaling pathway. J. Toxicol. Environ. Health A 68: 2005-2017