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Volumn 111, Issue 19, 2007, Pages 5062-5075

Functional metalloproteins integrated with conductive substrates: Detecting single molecules and sensing individual recognition events

Author keywords

[No Author keywords available]

Indexed keywords

BIOMATERIALS; BIOMOLECULES; ELECTRON TRANSITIONS; NANOTECHNOLOGY; REAL TIME SYSTEMS; REDOX REACTIONS; SUBSTRATES;

EID: 34249789018     PISSN: 15206106     EISSN: None     Source Type: Journal    
DOI: 10.1021/jp070035m     Document Type: Article
Times cited : (40)

References (131)
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    • Chen, R. J.; Bangsaruntip, S.; Drouvalakis, K. A.; Wong Shi Kam, N.; Shim, Yiming Li, M.; Kim, W.; Utz, P. J.; Dai, H. Proc. Natl. Acad. Sci. U.S.A. 2003, 100, 4984.
    • Chen, R. J.; Bangsaruntip, S.; Drouvalakis, K. A.; Wong Shi Kam, N.; Shim, Yiming Li, M.; Kim, W.; Utz, P. J.; Dai, H. Proc. Natl. Acad. Sci. U.S.A. 2003, 100, 4984.
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    • 0036509495 scopus 로고    scopus 로고
    • Andolfi, L.; Cannistraro, S.; Canters, G. W.; Facci, P.; Ficca, A. G.; Van, Amsterdam, I. M. C.; Verbeet, M. Ph. Arch. Biochem. Biophys. 2002, 399, 81.
    • Andolfi, L.; Cannistraro, S.; Canters, G. W.; Facci, P.; Ficca, A. G.; Van, Amsterdam, I. M. C.; Verbeet, M. Ph. Arch. Biochem. Biophys. 2002, 399, 81.
  • 59
    • 84906385140 scopus 로고    scopus 로고
    • The N-succinimidyl 3-maleimidopropionate (SMP) solution is prepared at 25 mM concentration in N,N-dimethylformamide (DMF). Full coverage of the Au(111) surface with maleimide groups is obtained by exposing the substrate to this solution for 40 min at room temperature.
    • The N-succinimidyl 3-maleimidopropionate (SMP) solution is prepared at 25 mM concentration in N,N-dimethylformamide (DMF). Full coverage of the Au(111) surface with maleimide groups is obtained by exposing the substrate to this solution for 40 min at room temperature.
  • 60
    • 84906413803 scopus 로고    scopus 로고
    • The N-succinimidyl-S-acetylthiopropionate (SATP) solution is prepared at 2 mM concentration in 10% dimethyl sulfoxide (DMSO) and 90% pH 7 phosphate-buffered saline (PBS) buffer. Room temperature incubation of the amino-terminated Au with this solution for 2 h provides full coverage of the Au surface with protected thiol groups. Deprotection is achieved by subsequent exposure of the modified substrate to a solution of 0.5 M hydroxylamine in pH 7 50 mM PBS, 25 mM ethylenediaminetetraacetic acid, and 50 mM dithiothreitol for 20 min. Samples were then rinsed with Milli-Q water (Millipore 18.2 MΩ).
    • The N-succinimidyl-S-acetylthiopropionate (SATP) solution is prepared at 2 mM concentration in 10% dimethyl sulfoxide (DMSO) and 90% pH 7 phosphate-buffered saline (PBS) buffer. Room temperature incubation of the amino-terminated Au with this solution for 2 h provides full coverage of the Au surface with protected thiol groups. Deprotection is achieved by subsequent exposure of the modified substrate to a solution of 0.5 M hydroxylamine in pH 7 50 mM PBS, 25 mM ethylenediaminetetraacetic acid, and 50 mM dithiothreitol for 20 min. Samples were then rinsed with Milli-Q water (Millipore 18.2 MΩ).
  • 66
    • 0004065793 scopus 로고
    • Marti, O, Ed, Academic Press Inc, San Diego, CA
    • Amrein, M. STM and SFM in Biology, Marti, O., Ed.; Academic Press Inc.: San Diego, CA, 1993.
    • (1993) STM and SFM in Biology
    • Amrein, M.1
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    • 0018101150 scopus 로고
    • Bell, G. I. Science 1978, 200, 618.
    • (1978) Science , vol.200 , pp. 618
    • Bell, G.I.1
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    • 84906370811 scopus 로고    scopus 로고
    • For details on tip functionalization with the spacer and following C551 incubation, see ref 110
    • For details on tip functionalization with the spacer and following C551 incubation, see ref 110.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.