Threshold Level of p53 Required for the Induction of Apoptosis in X-Irradiated MOLT-4 Cells

International Journal of Radiation Oncology Biology Physics

Volumn 68, Issue 3, 2007, Pages 883-891

  Nakano, Hisako ^a   Yonekawa, Hiromichi ^a   Shinohara, Kunio ^a,b  

^a TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE   (Japan)

^b JAPAN SYNCHROTRON RADIATION RESEARCH INSTITUTE   (Japan)

Author keywords

Apoptosis; Flow cytometry; MOLT 4 cells; p53 protein; Threshold level

Indexed keywords

CELL DEATH; DNA; DOSIMETRY; FLOW CYTOMETRY; X RAY ANALYSIS;

CELLULAR FREQUENCY DISTRIBUTION; DNA DAMAGE; MITOCHONDRIAL MEMBRANE POTENTIAL;

PROTEINS;

CASPASE 3; PROTEIN P53;

APOPTOSIS; ARTICLE; CELL DEATH; CONTROLLED STUDY; DNA DAMAGE; ENZYME ACTIVATION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MEMBRANE POTENTIAL; MITOCHONDRIAL MEMBRANE; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN LOCALIZATION; SIGNAL TRANSDUCTION; X IRRADIATION;

APOPTOSIS; APOPTOSIS REGULATORY PROTEINS; CELL LINE; DIFFERENTIAL THRESHOLD; DOSE-RESPONSE RELATIONSHIP, RADIATION; GENE EXPRESSION REGULATION; HUMANS; LEUKEMIA, T-CELL; RADIATION DOSAGE; TUMOR CELLS, CULTURED; TUMOR SUPPRESSOR PROTEIN P53;

EID: 34249282049     PISSN: 03603016     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijrobp.2007.03.007     Document Type: Article

References (42)

1. Levine A.J. p53, the cellular gatekeeper for growth and division. Cell 88 (1997) 323-331
2. Vogelstein B., Lane D., and Levine A.J. Surfing the p53 network. Nature 408 (2000) 307-310
3. Hollstein M., Sidransky D., Vogelstein B., et al. p53 mutations in human cancers. Science 253 (1991) 49-53
4. Greenblatt M.S., Bennett W.P., Hollstein M., et al. Mutations in the p53 tumor suppressor gene: Clues to cancer etiology and molecular pathogenesis. Cancer Res 54 (1994) 4855-4878
5. Laurie N.A., Donovan S.L., Shih C.S., et al. Inactivation of the p53 pathway in retinoblastoma. Nature 444 (2006) 61-66
6. Brooks C.L., and Gu W. p53 ubiquitination: MDM2 and beyond. Mol Cell 21 (2006) 307-315
7. Maki C.G., and Howley P.M. Ubiquitination of p53 and p21 is differentially affected by ionizing and UV radiation. Mol Cell Biol 17 (1997) 355-363
8. Lowe S.W., Schmitt E.M., Smith S.W., et al. p53 is required for radiation-induced apoptosis in mouse thymocytes. Nature 362 (1993) 847-849
9. Vousden K.H., and Lu X. Live or let die: The cell's response to p53. Nat Rev Cancer 2 (2002) 594-604
10. Villunger A., Michalak E.M., Coultas L., et al. p53- and drug-induced apoptotic responses mediated by BH3-only proteins puma and noxa. Science 302 (2003) 1036-1038
11. Chipuk J.E., Bouchier-Hayes L., Kuwana T., et al. PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. Science 309 (2005) 1732-1735
12. Chen X., Ko L.J., Jayaraman L., et al. p53 levels, functional domains, and DNA damage determine the extent of the apoptotic response of tumor cells. Genes Dev 10 (1996) 2438-2451
13. Shinohara K., Tomioka M., Nakano H., et al. Apoptosis induction resulting from proteasome inhibition. Biochem J 317 (1996) 385-388
14. Oda K., Arakawa H., Tanaka T., et al. p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell 102 (2000) 849-862
15. Zhao R., Gish K., Murphy M., et al. Analysis of p53-regulated gene expression patterns using oligonucleotide arrays. Genes Dev 14 (2000) 981-993
16. Kaeser M.D., and Iggo R.D. Chromatin immunoprecipitation analysis fails to support the latency model for regulation of p53 DNA binding activity in vivo. Proc Natl Acad Sci U S A 99 (2002) 95-100
17. Vassilev L.T., Vu B.T., Graves B., et al. In vivo activation of the p53 pathway by small-molecule antagonists of MDM2. Science 303 (2004) 844-848
18. Lane D. How cells choose to die. Nature 414 (2001) 25-27
19. Minowada J., Ohnuma T., and Moore G.E. Rosette-forming human lymphoid cell lines. 1. Establishment and evidence for origin of thymus-derived lymphocytes. J Natl Cancer Inst 49 (1972) 891-895
20. Han T., Pauly J.L., and Minowada J. In vitro preferential effect of irradiation on cultured T lymphoid cell line. Clin Exp Immunol 17 (1974) 455-462
21. Shinohara K., and Nakano H. Interphase death and reproductive death in X-irradiated MOLT-4 cells. Radiat Res 135 (1993) 197-205
22. Nakano H., and Shinohara K. X-ray-induced cell death: Apoptosis and necrosis. Radiat Res 140 (1994) 1-9
23. Nakano H., and Shinohara K. Correlation between unirradiated cell TP53 protein levels and radiosensitivity in MOLT-4 cells. Radiat Res 151 (1999) 686-693
24. Nakano H., Kohara M., and Shinohara K. Evaluation of the relative contribution of p53-mediated pathway in X-ray-induced apoptosis in human leukemic MOLT-4 cells by transfection with a mutant p53 gene at different expression levels. Cell Tissue Res 306 (2001) 101-106
25. Greenberg J.M., Gonzalez-Sarmiento R., Arthur D.C., et al. Immunophenotypic and cytogenetic analysis of Molt-3 and Molt-4: Human T-lymphoid cell lines with rearrangement of chromosome 7. Blood 72 (1988) 1755-1760
26. Zhao Q.L., Kondo T., Noda A., et al. Mitochondrial and intracellular free-calcium regulation of radiation-induced apoptosis in human leukemic cells. Int J Radiat Biol 75 (1999) 493-504
27. Nakano H., Yonekawa H., and Shinohara K. Delayed expression of apoptosis in X-irradiated human leukemic MOLT-4 cells transfected with mutant p53. J Radiat Res 44 (2003) 179-183
28. Crosby M.E., Oancea M., and Almasan A. p53 binding to target sites is dynamically regulated before and after ionizing radiation-mediated DNA damage. J Environ Pathol Toxicol Oncol 23 (2004) 67-79
29. Gong B., and Almasan A. Differential upregulation of p53-responsive genes by genotoxic stress in hematopoietic cells containing wild-type and mutant p53. Gene Expr 8 (1999) 197-206
30. Gong B., Chen Q., Endlich B., et al. Ionizing radiation-induced, Bax-mediated cell death is dependent on activation of cysteine and serine proteases. Cell Growth Differ 10 (1999) 491-502
31. Morita A., Zhu J., Suzuki N., et al. Sodium orthovanadate suppresses DNA damage-induced caspase activation and apoptosis by inactivating p53. Cell Death Differ 13 (2006) 499-511
32. Enomoto A., Suzuki N., Hirano K., et al. Involvement of SAPK/JNK pathway in X-ray-induced rapid cell death of human T-cell leukemia cell line MOLT-4. Cancer Lett 155 (2000) 137-144
33. Nakano H., and Shinohara K. Time sequence analysis of caspase-3-independent programmed cell death and apoptosis in X-irradiated human leukemic MOLT-4 cells. Cell Tissue Res 310 (2002) 305-311
34. Chehab N.H., Malikzay A., Stavridi E.S., et al. Phosphorylation of Ser-20 mediates stabilization of human p53 in response to DNA damage. Proc Natl Acad Sci U S A 96 (1999) 13777-13782
35. Quillet-Mary A., Jaffrezou J.P., Mansat V., et al. Implication of mitochondrial hydrogen peroxide generation in ceramide-induced apoptosis. J Biol Chem 272 (1997) 21388-21395
36. Filippini G., Griffin S., Uhr M., et al. A novel flow cytometric method for the quantification of p53 gene expression. Cytometry 31 (1998) 180-186
37. Healy E., Dempsey M., Lally C., et al. Apoptosis and necrosis: Mechanisms of cell death induced by cyclosporine A in a renal proximal tubular cell line. Kidney Int 54 (1998) 1955-1966
38. Pheng S., Chakrabarti S., and Lamontagne L. Dose-dependent apoptosis induced by low concentrations of methylmercury in murine splenic Fas+ T cell subsets. Toxicology 149 (2000) 115-128
39. Yonish-Rouach E., Resnitzky D., Lotem J., et al. Wild-type p53 induces apoptosis of myeloid leukaemic cells that is inhibited by interleukin-6. Nature 352 (1991) 345-347
40. Shaw P., Bovey R., Tardy S., et al. Induction of apoptosis by wild-type p53 in a human colon tumor-derived cell line. Proc Natl Acad Sci U S A 89 (1992) 4495-4499
41. Thompson T., Tovar C., Yang H., et al. Phosphorylation of p53 on key serines is dispensable for transcriptional activation and apoptosis. J Biol Chem 279 (2004) 53015-53022
42. Dumont P., Leu J.I., Della Pietra III A.C., et al. The codon 72 polymorphic variants of p53 have markedly different apoptotic potential. Nat Genet 33 (2003) 357-365