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Volumn 316, Issue 5823, 2007, Pages 449-453

Raman-assisted crystallography reveals end-on peroxide intermediates in a nonheme iron enzyme

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME; HYDROGEN PEROXIDE; IRON; PEROXIDE; SCAVENGER; SUPEROXIDE; SUPEROXIDE REDUCTASE; UNCLASSIFIED DRUG;

EID: 34247525538     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1138885     Document Type: Article
Times cited : (133)

References (32)
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    • G. I. Berglund et al., Nature 417, 463 (2002).
    • (2002) Nature , vol.417 , pp. 463
    • Berglund, G.I.1
  • 8
    • 0347264753 scopus 로고    scopus 로고
    • A. Karlsson et al., Science 299, 1039 (2003).
    • (2003) Science , vol.299 , pp. 1039
    • Karlsson, A.1
  • 24
    • 34247503613 scopus 로고    scopus 로고
    • Materials and methods are available as supporting material on Science Online.
    • Materials and methods are available as supporting material on Science Online.
  • 25
    • 34247471086 scopus 로고    scopus 로고
    • Raman experiments were performed under nonresonant conditions, taking advantage of the large protein concentration found in crystals to enhance the signal-to-noise ratio. In this way, the crystals were sampled homogeneously, and potential light-induced damage or photochemistry that could develop under resonant conditions was avoided
    • Raman experiments were performed under nonresonant conditions, taking advantage of the large protein concentration found in crystals to enhance the signal-to-noise ratio. In this way, the crystals were sampled homogeneously, and potential light-induced damage or photochemistry that could develop under resonant conditions was avoided.
  • 26
    • 34247538300 scopus 로고    scopus 로고
    • J.-J. Girerd, F. Banse, A. J. Simaan, in Metal-Oxo and Metal-Peroxo Species in Catalytic Oxidations, 97 of Structure and Bonding (Springer, Berlin 2000), pp. 145-177.
    • J.-J. Girerd, F. Banse, A. J. Simaan, in Metal-Oxo and Metal-Peroxo Species in Catalytic Oxidations, vol. 97 of Structure and Bonding (Springer, Berlin 2000), pp. 145-177.
  • 27
    • 34247514269 scopus 로고    scopus 로고
    • A carefully controlled x-ray exposure was used to collect the diffraction data so that the dose absorbed by the crystal amounted to ∼1% of the Henderson limit, i.e, ∼2.1 × 105 gray Gy
    • 5 gray (Gy).
  • 28
    • 34247546991 scopus 로고    scopus 로고
    • δ) to a close distance (3.5 Å) to the distal oxygen of the hydroperoxo moiety.
    • δ) to a close distance (3.5 Å) to the distal oxygen of the hydroperoxo moiety.
  • 32
    • 34247523611 scopus 로고    scopus 로고
    • Coordinates and structure factor amplitudes of the structures SOR E114A, Fe(II, SORE114A, Fe(III)-OOH and SOR wt, Fe(II) have been deposited in the RCSB Protein Data Bank with pdb codes 2ji2, 2ji3, and 2ji1, respectively. The ESRF is acknowledged for continuous support of our methodological developments. We are grateful to the ESRF beamline staff at ID14-1, ID14-2, ID14-4, and ID29 for their expert assistance; T. Mattioli and C. Mathé for insightful discussions and for providing us with resonance Raman spectra on the E114A SOR mutant; C. Mathevon for help in sample preparation; M. F. Field and L. David for help with the pKa calculations; J. C. Fontecilla for reading the manuscript; and H. Jouve and J.-P. Mahy for providing us with H2 18O2. G.K. acknowledges support by a European Molecular Biology Organization long-term fellowship, and D.B. acknowledges support by the Contrat de Plan Etat-Ré
    • 2. G.K. acknowledges support by a European Molecular Biology Organization long-term fellowship, and D.B. acknowledges support by the Contrat de Plan Etat-Région and the Action Concertée Incitative programs from the French Ministry of Research. V.N. acknowledges support by the Toxicologie Nucléaire program from the CEA.


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