Rho-kinase inhibition acutely augments blood flow in focal cerebral ischemia via endothelial mechanisms

Journal of Cerebral Blood Flow and Metabolism

Volumn 27, Issue 5, 2007, Pages 998-1009

  Shin, Hwa Kyoung ^a   Salomone, Salvatore ^a   Potts, E Michelle ^a   Lee, Sae Won ^a   Millican, Eric ^b   Noma, Kensuke ^b   Huang, Paul L ^a   Boas, David A ^a   Liao, James K ^b   Moskowitz, Michael A ^a   Ayata, Cenk ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b BRIGHAM AND WOMEN S HOSPITAL   (United States)

Author keywords

Focal cerebral ischemia; Hydroxyfasudil; Hypoxia; Isolated vessels; Laser speckle flowmetry; Rho kinase

Indexed keywords

4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE; ACETYLCHOLINE; ENDOTHELIAL NITRIC OXIDE SYNTHASE; FASUDIL; HYDRALAZINE; N5 (1 IMINOETHYL)ORNITHINE; RHO KINASE; RHO KINASE INHIBITOR;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BRAIN BLOOD FLOW; BRAIN CORTEX; BRAIN HYPOXIA; BRAIN INFARCTION SIZE; BRAIN ISCHEMIA; CHRONOTHERAPY; COMBINATION CHEMOTHERAPY; CONTINUOUS INFUSION; CONTROLLED STUDY; DISEASE EXACERBATION; DISEASE SEVERITY; DRUG MECHANISM; ENZYME ACTIVATION; ENZYME INHIBITION; ENZYME REGULATION; FLOW MEASUREMENT; HEMODYNAMICS; IN VIVO CULTURE; MIDDLE CEREBRAL ARTERY OCCLUSION; MONOTHERAPY; MOUSE; NONHUMAN; PRIORITY JOURNAL; RAT; VASCULAR ENDOTHELIUM; VASCULAR SMOOTH MUSCLE; VASODILATATION; WILD TYPE;

1-(5-ISOQUINOLINESULFONYL)-2-METHYLPIPERAZINE; ACETYLCHOLINE; ANIMALS; BRAIN ISCHEMIA; CEREBROVASCULAR CIRCULATION; DOWN-REGULATION; ENDOTHELIUM, VASCULAR; ENZYME INHIBITORS; HYPOXIA, BRAIN; INFARCTION, MIDDLE CEREBRAL ARTERY; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; LASER-DOPPLER FLOWMETRY; MICE; MICE, INBRED C57BL; MICE, KNOCKOUT; NG-NITROARGININE METHYL ESTER; NITRIC OXIDE SYNTHASE TYPE III; PROTEIN-SERINE-THREONINE KINASES; RATS; STIMULATION, CHEMICAL; VASODILATOR AGENTS;

EID: 34247491041     PISSN: 0271678X     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.jcbfm.9600406     Document Type: Article

References (38)

1. Amano M, Fukata Y, Kaibuchi K (2000) Regulation and functions of Rho-associated kinase. Exp Cell Res 261:44-51
2. Amin-Hanjani S, Stagliano NE, Yamada M, Huang PL, Liao JK, Moskowitz MA (2001) Mevastatin, an HMG-CoA reductase inhibitor, reduces stroke damage and upregulates endothelial nitric oxide synthase in mice. Stroke 32:980-6
3. Arai M, Sasaki Y, Nozawa R (1993) Inhibition by the protein kinase inhibitor HA1077 of the activation of NADPH oxidase in human neutrophils. Biochem Pharmacol 46:1487-90
4. Ayata C, Dunn AK, Gursoy OY, Huang Z, Boas DA, Moskowitz MA (2004) Laser speckle flowmetry for the study of cerebrovascular physiology in normal and ischemic mouse cortex. J Cereb Blood Flow Metab 24:744-55
5. Bao W, Hu E, Tao L, Boyce R, Mirabile R, Thudium DT, Ma XL, Willette RN, Yue TL (2004) Inhibition of Rho-kinase protects the heart against ischemia/reperfusion injury. Cardiovasc Res 61:548-58
6. Endres M (2005) Statins and stroke. J Cereb Blood Flow Metab 25:1093-110
7. Endres M, Laufs U, Huang Z, Nakamura T, Huang P, Moskowitz MA, Liao JK (1998) Stroke protection by 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase inhibitors mediated by endothelial nitric oxide synthase. Proc Natl Acad Sci USA 95:8880-5
8. Fagan KA, Oka M, Bauer NR, Gebb SA, Ivy DD, Morris KG, McMurtry IF (2004) Attenuation of acute hypoxic pulmonary vasoconstriction and hypoxic pulmonary hypertension in mice by inhibition of Rho-kinase. Am J Physiol Lung Cell Mol Physiol 287:L656-64
9. Fulton D, Gratton JP, McCabe TJ, Fontana J, Fujio Y, Walsh K, Franke TF, Papapetropoulos A, Sessa WC (1999) Regulation of endothelium-derived nitric oxide production by the protein kinase Akt. Nature 399:597-601
10. Hitomi A, Satoh S, Ikegaki I, Suzuki Y, Shibuya M, Asano T (2000) Hemorheological abnormalities in experimental cerebral ischemia and effects of protein kinase inhibitor on blood fluidity. Life Sci 67:1929-39
11. Huang PL, Huang Z, Mashimo H, Bloch KD, Moskowitz MA, Bevan JA, Fishman MC (1995) Hypertension in mice lacking the gene for endothelial nitric oxide synthase. Nature 377:239-42
12. Huang Z, Huang PL, Ma J, Meng W, Ayata C, Fishman MC, Moskowitz MA (1996) Enlarged infarcts in endothelial nitric oxide synthase knockout mice are attenuated by nitro-L-arginine. J Cereb Blood Flow Metab 16:981-7
13. Kawano Y, Fukata Y, Oshiro N, Amano M, Nakamura T, Ito M, Matsumura F, Inagaki M, Kaibuchi K (1999) Phosphorylation of myosin-binding subunit (MBS) of myosin phosphatase by Rho-kinase in vivo. J Cell Biol 147:1023-38
14. Kimura K, Ito M, Amano M, Chihara K, Fukata Y, Nakafuku M, Yamamori B, Feng J, Nakano T, Okawa K, Iwamatsu A, Kaibuchi K (1996) Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase). Science 273:245-8
15. Laufs U, Liao JK (1998) Post-transcriptional regulation of endothelial nitric oxide synthase mRNA stability by Rho GTPase. J Biol Chem 273:24266-71
16. Liao JK, Zulueta JJ, Yu FS, Peng HB, Cote CG, Hassoun PM (1995) Regulation of bovine endothelial constitutive nitric oxide synthase by oxygen. J Clin Invest 96:2661-6
17. Limbourg FP, Huang Z, Plumier JC, Simoncini T, Fujioka M, Tuckermann J, Schutz G, Moskowitz MA, Liao JK (2002) Rapid nontranscriptional activation of endothelial nitric oxide synthase mediates increased cerebral blood flow and stroke protection by corticosteroids. J Clin Invest 110:1729-38
18. Ming XF, Viswambharan H, Barandier C, Ruffieux J, Kaibuchi K, Rusconi S, Yang Z (2002) Rho GTPase/Rho kinase negatively regulates endothelial nitric oxide synthase phosphorylation through the inhibition of protein kinase B/Akt in human endothelial cells. Mol Cell Biol 22:8467-77
19. Noma K, Oyama N, Liao JK (2006) Physiological role of ROCKs in the cardiovascular system. Am J Physiol Cell Physiol 290:C661-8
20. Rikitake Y, Kim HH, Huang Z, Seto M, Yano K, Asano T, Moskowitz MA, Liao JK (2005) Inhibition of Rho kinase (ROCK) leads to increased cerebral blood flow and stroke protection. Stroke 36:2251-7
21. Rikitake Y, Liao JK (2005) Rho-kinase mediates hyperglycemia-induced plasminogen activator inhibitor-1 expression in vascular endothelial cells. Circulation 111:3261-8
22. Satoh S, Ikegaki I, Suzuki Y, Asano T, Shibuya M, Hidaka H (1996) Neuroprotective properties of a protein kinase inhibitor against ischaemia-induced neuronal damage in rats and gerbils. Br J Pharmacol 118:1592-6
23. Satoh S, Kobayashi T, Hitomi A, Ikegaki I, Suzuki Y, Shibuya M, Yoshida J, Asano T (1999) Inhibition of neutrophil migration by a protein kinase inhibitor for the treatment of ischemic brain infarction. Jpn J Pharmacol 80:41-8
24. Satoh S, Utsunomiya T, Tsurui K, Kobayashi T, Ikegaki I, Sasaki Y, Asano T (2001) Pharmacological profile of hydroxy fasudil as a selective rho kinase inhibitor on ischemic brain damage. Life Sci 69:1441-53
25. Seko T, Ito M, Kureishi Y, Okamoto R, Moriki N, Onishi K, Isaka N, Hartshorne DJ, Nakano T (2003) Activation of RhoA and inhibition of myosin phosphatase as important components in hypertension in vascular smooth muscle. Circ Res 92:411-8
26. Shah DI, Singh M (2006) Involvement of Rho-kinase in experimental vascular endothelial dysfunction. Mol Cell Biochem 283:191-9
27. Shimokawa H (2002) Rho-kinase as a novel therapeutic target in treatment of cardiovascular diseases. J Cardiovasc Pharmacol 39:319-27
28. Shin HK, Dunn AK, Jones PB, Boas DA, Moskowitz MA, Ayata C (2006) Vasoconstrictive neurovascular coupling during focal ischemic depolarizations. J Cereb Blood Flow Metab 26:1018-30
29. Takahara A, Sugiyama A, Satoh Y, Yoneyama M, Hashimoto K (2003) Cardiovascular effects of Y-27632, a selective Rho-associated kinase inhibitor, assessed in the halothane-anesthetized canine model. Eur J Pharmacol 460:51-7
30. Takemoto M, Sun J, Hiroki J, Shimokawa H, Liao JK (2002) Rho-kinase mediates hypoxia-induced downregulation of endothelial nitric oxide synthase. Circulation 106:57-62
31. Terzic A, Kurachi Y (1996) Actin microfilament disrupters enhance K(ATP) channel opening in patches from guinea-pig cardiomyocytes. J Physiol 492(Part 2):395-404
32. Toshima Y, Satoh S, Ikegaki I, Asano T (2000) A new model of cerebral microthrombosis in rats and the neuroprotective effect of a Rho-kinase inhibitor. Stroke 31:2245-50
33. Uehata M, Ishizaki T, Satoh H, Ono T, Kawahara T, Morishita T, Tamakawa H, Yamagami K, Inui J, Maekawa M, Narumiya S (1997) Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension. Nature 389:990-4
34. Vedernikov YP, Graser T, Leisner H, Tiedt N (1991) Effect of hypoxia on endothelium-dependent relaxation of porcine coronary arteries and veins. Biomed Biochim Acta 50:257-63
35. Wolfrum S, Dendorfer A, Rikitake Y, Stalker TJ, Gong Y, Scalia R, Dominiak P, Liao JK (2004) Inhibition of Rho-kinase leads to rapid activation of phosphatidylinositol 3-kinase/protein kinase Akt and cardiovascular protection. Arterioscler Thromb Vasc Biol 24:1842-7
36. Yagita Y, Kitagawa K, Sasaki T, Sugiura S, Todo K, Omura-Matsuoka E, Matsushita K, Hori M (2005) Activation of Rho/Rho kinase system after focal cerebral ischemia. J Cereb Blood Flow Metab 25:S264
37. Zhang F, White JG, Iadecola C (1994) Nitric oxide donors increase blood flow and reduce brain damage in focal ischemia: evidence that nitric oxide is beneficial in the early stages of cerebral ischemia. J Cereb Blood Flow Metab 14:217-26
38. Zhu Y, Liao HL, Niu XL, Yuan Y, Lin T, Verna L, Stemerman MB (2003) Low density lipoprotein induces eNOS translocation to membrane caveolae: the role of RhoA activation and stress fiber formation. Biochim Biophys Acta 1635:117-26