A comparison of homogeneous bioluminescent and fluorescent methods for protease assays

Handbook of Assay Development in Drug Discovery

Volumn , Issue , 2006, Pages 125-139

  O'Brien, Martha A ^a  

^a PROMEGA CORPORATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 34147222777     PISSN: None     EISSN: None     Source Type: Book    
DOI: None     Document Type: Chapter

References (22)

1. Leytus SP, Melhado LL, Mangel WF. Rhodamine-based compounds as fluorogenic substrates for serine proteinases. Biochem J 1983;209:299-307.
2. Liu J, Bhalgat M, Zhang C, Diwu Z, Hoyland B, Klaubert DH. Fluorescent molecular probes V: a sensitive caspase-3 substrate for fluorometric assays. Bioorg Med Chem Lett 1999;9:3231-3236.
3. Grant SK, Sklar JG, Cummings RT. Development of novel assays for proteolytic enzymes using rhodamine-based fluorogenic substrates. J Biomol Screen 2002;7:531-540.
4. Monsees T, Miska W, Geiger R. Synthesis and characterization of a bioluminogenic substrate for alpha-chymotrypsin. Anal Biochem 1994;221:329-334.
5. Monsees T, Geiger R, Miska W. A novel bioluminogenic assay for alpha-chymotrypsin. J Biolumin Chemilumin 1995;10:213-218.
6. O'Brien MA, Daily WJ, Hesselberth PE, Moravec RA, Scurria MA, Klaubert DH, Bulleit RF, Wood KV. Homogeneous, bioluminescent protease assays: caspase-3 as a model. J Biomed Screen 2005;10:137-148.
7. Hall MP, Gruber MG, Hannah RR, Jennens-Clough ML, Wood KV. Stabilization of firefly luciferase using directed evolution. In: Roda, A. et al., (eds.): Bioluminescence and Chemiluminescence - Perspectives for the 21st Century. Chichester, U.K.: John Wiley & Sons, 1998:392-395.
8. Zhang JH, Chung TD, Oldenburg KR. A simple statistical parameter for use in evaluation and validation of high-throughput screening assays. J Biomol Screen 1999;4:67-73.
9. Tawa P, Tam J, Cassady R, Nicholson DW, Xanthoudakis S. Quantitative analysis of fluorescent caspase substrate cleavage in intact cells and identification of novel inhibitors of apoptosis. Cell Death Differ 2001;8:30-37.
10. Waud JP, Bermudez Fajardo A, Sudhaharan T, Trimby AR, Jeffery J, Jones A, Campbell AK. Measurement of proteases using chemiluminescence-resonance-energy-transfer chimaeras between green fluorescent protein and aequorin. Biochem J 2001;357:687-697.
11. Preaudat M, Ouled-Diaf J, Alpha-Bazin B, Mathis G, Mitsugi T, Aono Y, Takahashi K, Takemoto H. A homogeneous caspase-3 activity assay using HTRF technology. J Biomol Screen 2002;7:267-274.
12. Karvinen J, Hurskainen P, Gopalakrishnan S, Burns D, Warrior U, Hemmila I. Homogeneous timeresolved fluorescence quenching assay (LANCE) for caspase-3. J Biomol Screen 2002;7:223-231.
13. Gopalakrishnan SM, Karvinen J, Kofron JL, Burns DJ, Warrior U. Application of micro arrayed compound screening (^ARCS) to identify inhibitors of caspase-3. J Biomol Screen 2002;7:317-323.
14. Thornberry NA, Rano TA, Peterson EP, Rasper DM, Timkey T, Garcia-Calvo M, Houtzager VM, Nordstrom PA, Roy S, Vaillancourt JP, Chapman KT, Nicholson DW. A combinatorial approach defines specificities of members of the caspase family and granzyme B. J Biol Chem 1997;272:17,907-17,911.
15. Niles A, Moravec R, Scurria M, O'Brien M, Riss T. A novel homogeneous bioluminescent caspase-8 activity assay [Poster 274]. Paper presented at the Keystone Symposia: Molecular Mechanisms of Apoptosis, Banff, Alberta, February 2003.
16. Wiederman, PE, Trevillyan JM. Dipeptidyl peptidase IV inhibitors for the treatment of impaired glucose tolerance and type 2 diabetes. Curr Opinion Invest Drugs 2003;4:412-420.
17. Goll DE, Thompson VF, Li H, Wei W, Cong J. The calpain system. Physiol Rev 2003:83:731-801.
18. Sasaki T, Kikuchi T, Yumoto N, Yoshimura N, Murachi T. Comparative specificity and kinetic studies on porcine calpain I and calpain II with naturally occurring peptides and synthetic fluorogenic substrates. J Biol Chem 1984;259:12,489-12,494.
19. Tompa P, Schád E, Baki A, Alexa A, Batke J, Freidrich P. An ultrasensitive, continuous fluorometric assay for calpain activity. Anal Biochem 1995;228:287-293.
20. Thompson VF, Saldana S, Cong J, Goll DE. A BODIPY fluorescent microplate assay for measuring activity of calpains and other proteases. Anal Biochem 2000;279:170-178.
21. Mittoo S, Sundstrom LE, Bradley M. Synthesis and evaluation of fluorescent probes for the detection of calpain activity. Anal Biochem 2003;319:234-238.
22. Li H, Thompson VF, Goll DE. Effects of autolysis on properties of \i- and m-calpain. Biochim Biophys Acta 2004;1691:91-103.