Fluorescent molecular probes V: A sensitive caspase-3 substrate for fluorometric assays

Bioorganic and Medicinal Chemistry Letters

Volumn 9, Issue 22, 1999, Pages 3231-3236

  Liu, Jixiang ^a   Bhalgat, Mahesh ^a   Zhang, Cailan ^a   Diwu, Zhenjun ^a   Hoyland, Brian ^a   Klaubert, Dieter H ^a  

^a MOLECULAR PROBES INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CASPASE 3; RHODAMINE 110;

ARTICLE; CHEMICAL MODIFICATION; ENZYME ACTIVITY; ENZYME SUBSTRATE; FLOW CYTOMETRY; FLUOROMETRY; MOLECULAR PROBE; STRUCTURE ANALYSIS; SYNTHESIS;

EID: 0033571424     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/s0960-894x(99)00566-1     Document Type: Article

References (8)

1. Keppler-Hafkemeyer, A.; Brinkmann, U.; Pastan, I. Biochemistry 1998, 37, 16934.
2. Kumar, S. Clin. Exp. Pharmacol. Physiol. 1999, 26, 295.
3. Porter, A. G.; Janicke, R. U. Cell Death Differ. 1999, 6, 99.
4. Stennicke, H. R.; Salvesen, G. S. J. Biol. Chem. 1997, 272, 25719.
5. 4OAc (pH = 7); 65 mL/min flow rate. The structure was confirmed to elemental analysis, mass and NMR spectra.
6. Purified active recombinant human caspase-3 (Pharmingen, San Diego, CA) was used in the microplate assays.
7. Diwu, Z.; Lu, Y.; Zhang, C.; Klaubert, D. H. Photochem. Photobiol. 1997, 66, 424.
8. Jurkat cells (American Type Culture Collection Co., Rockville, MD) were used in our flow cytometric assays. 10 μM camptothecin was used to induce apoptosis.