Homogeneous time-resolved fluorescence quenching assay (LANCE) for caspase-3

Journal of Biomolecular Screening

Volumn 7, Issue 3, 2002, Pages 223-231

  Karvinen, Jarkko ^a   Hurskainen, Pertti ^a   Gopalakrishnan, Sujatha ^b   Burns, David ^a   Warrior, Usha ^a   Hemmilä, Ilkka ^a  

^a ABBOTT LABORATORIES   (United States)

^b PerkinElmer Finland Oy   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

CASPASE 3; CHELATE; EUROPIUM; G PROTEIN COUPLED RECEPTOR; PHOSPHOTRANSFERASE; PROTEINASE; CASPASE;

ARTICLE; CELL LYSATE; ENZYME ASSAY; FLUORESCENCE ANALYSIS; INTERMETHOD COMPARISON; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN TARGETING; SENSITIVITY AND SPECIFICITY; TECHNOLOGY; BIOASSAY; FLUORESCENCE; METABOLISM;

BIOLOGICAL ASSAY; CASPASES; EUROPIUM; FLUORESCENCE; CASPASE 3;

EID: 0035991320     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1089/108705702760047727     Document Type: Article

References (22)

1. Caspases: Key mediators of apoptosis
2. The role of the caspase family of cysteine proteases in apoptosis
3. Macromolecular substrates for the ICE-like proteases during apoptosis
4. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis
5. Fluorometric and colorimetric detection of caspase activity associated with apoptosis
6. Fluorescent molecular probes V: A sensitive caspase-3 substrate for fluorometric assays
7. Development and application of a GFP-FRET intracellular caspase assay for drug screening
8. Synthesis of a fluorogenic interleukin-1β converting enzyme substrate based on resonance energy transfer
9. LANCE™ vs. HTRF® technologies (or vice versa)
10. LANCE™: Homogeneous assay platform for HTS
11. HTRF® technology
12. Rare earth cryptates and homogeneous fluoroimmunoassays with human sera
13. Synthesis of europium(III) chelates suitable for labeling of bioactive molecules
14. Development of luminescent lanthanide chelate labels for diagnostic assays
15. Biochemical characteristics of caspases-3, -6, -7 and -8
16. A simple statistical parameter for use in evaluation and validation of high throughput screening
17. Novel fluorogenic substrates for assaying retroviral proteases by resonance energy transfer
18. Homogeneous fluorescence readouts for miniaturized high-throughput screening: Theory and practice
19. Continuous format high throughput screening for identification of inhibitors of caspase-3
20. Evaluation of different FRET pairs for metalloproteinase substrates
21. Time-resolved fluorescence energy transfer DNA helicase assay for high throughput screening