High level expression of recombinant Mycobacterium tuberculosis culture filtrate protein CFP32 in Pichia pastoris

Molecular Biotechnology

Volumn 35, Issue 1, 2007, Pages 41-49

  Benabdesselem, C ^a   Barbouche, M R ^a   Jarboui, M A ^a   Dellagi, K ^a   Ho, J L ^b   Fathallah, D M ^a  

^a PASTEUR INSTITUTE OF TUNIS   (Tunisia)

^b WEILL CORNELL MEDICAL COLLEGE   (United States)

Author keywords

Culture filtrate protein; Mycobacterium tuberculosis; Pichia pastoris; Recombinant

Indexed keywords

CULTURE FILTRATE PROTEIN; MYCOBACTERIUM TUBERCULOSIS; PICHIA PASTORIS; POSTTRANSLATIONAL MODIFICATIONS;

DISEASES; ESCHERICHIA COLI; GENES; GLYCOSYLATION; VACCINES; YEAST;

PROTEINS;

BACTERIAL PROTEIN; BCG VACCINE; CULTURE FILTRATE PROTEIN 32; HYBRID PROTEIN; MYCOBACTERIUM ANTIGEN; RECOMBINANT PROTEIN; SIGNAL PEPTIDE; UNCLASSIFIED DRUG;

ACYLATION; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CHEMICAL REACTION; CONTROLLED STUDY; DIAGNOSTIC VALUE; GENE EXPRESSION; GLYCOSYLATION; HUMAN; MOLECULAR CLONING; MOLECULAR WEIGHT; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PICHIA PASTORIS; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEIN SECRETION; SERODIAGNOSIS; TUBERCULOSIS;

AMINO ACID SEQUENCE; ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; BASE SEQUENCE; BIOTECHNOLOGY; CLONING, MOLECULAR; DNA, BACTERIAL; GENE EXPRESSION; GENES, BACTERIAL; HUMANS; MYCOBACTERIUM TUBERCULOSIS; PICHIA; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT FUSION PROTEINS; SEROLOGIC TESTS; TUBERCULOSIS;

ESCHERICHIA COLI; MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS; PICHIA PASTORIS;

EID: 34147109280     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/MB:35:1:41     Document Type: Article

References (31)

1. Cole, S. T., Brosch, R., Parkhill, J., et al. (1998) Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393, 537-544.
2. Bassey, E. O. E., Catty, D., Kumararatne, D. S., and Raykundalia, C. (1996) Candidate antigens for improved serodiagnosis of tuberculosis. Tubercle and Lung Disease 77, 136-145.
3. Weldingh, K., Rosenkrands, I., Okkels, L. M., Doherty, T. M., and Andersen, P. (2005) Assessing the serodiagnostic potential of 35 Mycobacterium tuberculosis proteins and identification of four novel serological antigens. J. Clin. Microb. 43, 57-65.
4. Huard, R. C., Lazzarini, L. C., Buttler, W. R., Van Soolingen, D., and Ho, J. L. (2003) PCR-Based method to differentiate the subspecies of Mycobacterium tuberculosis complex on the basis genomic deletions. J. Clin. Microb. 41, 1637-1650.
5. RosenKrands, I., Weldingh, K., Jacobsen, S., et al.(2000) Mapping and identification of Mycobacterium tuberculosis proteins by two dimensional gel electrophoresis, microsequencing and immunodetection. Electrophoresis 2, 935-949.
6. RosenKrands, I., King, A., Weldingh, K., Moniatte, M., Moertz, E., and Andersen, P. (2000) Towards the proteome of Mycobacterium tuberculosis. Electrophoresis 21, 3740-3756.
7. Huard, R. C., Chitale, S., Leung, M., et al. (2003). The Mycobacterium tuberculosis complex-restricted gene cfp32 encodes an expressed protein that is detectable in tuberculosis patients and is positively correlated with pulmonary interleukin-10. Infect. Immunol. 71, 6871-6883.
8. Andreu, N., Ysoto C., Roca, I., Martin, C., and Gibert, I. (2004) Mycobacterium smegmatis displays the Mycobacterium tuberculosis virulence-related neural red character when expressing the Rv0577 gene. FEMS Microb. Lett. 231, 283-289.
9. Korepanova, A., Gao, F. P., Hua, Y., Qin, H., Nakamoto, R. K., and Cross, T. A. (2005) Cloning and expression of multiple integral membrane proteins from Mycobacterium tuberculosis in Escherichia coli. Prot. Sci. 14, 148-158.
10. Choudhary, R. K., Pullakhandam, R., Ehtesham, N. Z., and Hasnain, S. E. (2004) Expression and characterization of Rv2430c, a novel immunodominant antigen of Mycobacterium tuberculosis. Prot. Expr. Purif. 36, 249-253.
11. Poletto, S. S., Da Fonseca, I. O., De Carvalho, L. P., Basso, L. A., and Santos, D. S. (2004) Selection of an Escherichia coli host that expresses mutant forms of Mycobacterium tuberculosis 2-trans enoyl-ACP(CoA) reductase and 3-ketoacyl-ACP(CoA) reductase enzymes. Prot. Expr. Purif. 34, 118-125.
12. Zhang, Y., Lathigra, R., Garbe, T., Catty, D., and Young, D. (1991) Genetic analysis of superoxide dismutase, the 23 kilodalton antigen of Mycobacterium tuberculosis. Mol. Microbiol. 5, 381-391.
13. Andersson, G. E. and Sharp, P. M. (1996) Codon usage in the Mycobacterium tuberculosis complex. Microbiology 142, 915-925.
14. Lakey, D. L., Rama K. R. Voladri, K. M., et al. (2000) Enhanced production of recombinant Mycobacterium tuberculosis antigens in Escherichia coli by replacement of low usage codons. Infect. Immunity 68, 233-238.
15. Atkins, D., Al-Ghussein, H., Prehaund, C., and Contes, A. R. (1994) Overproduction and purification of Mycobacterium tuberculosis chaperonin 10. Gene 150, 145-148.
16. Bigi, F., Taboga, O., Romano, M. I., Alito, A., Fisanotti, J. C., and Cataldi, A. A. (1999) Expression of the Mycobacterium bovis P36 gene in Mycobacterium smegmatis and the baculovirus/insect cell system. Braz. J. Med. Biol. Res. 32, 29-37.
17. Lara, M., Servin-Gonzalez, L., Singh, M., et al. (2004) Expression, secretion, and glycosylation of the 45- and 47-kDa glycoprotein of Mycobacterium tuberculosis in Streptomyces lividans. Appl. Environ. Microbiol. 70, 679-685.
18. Salim, K., Haedens, V., Content, J., Leblon, G., and Huygen, K. (1997) Heterologous expression of the Mycobacterium tuberculosis gene encoding antigen 85A in Corynebacterium glutamicum. Appl. Environ. Microbiol. 63, 4392-4400.
19. Dale, J. W. and Patki A. (1990) Mycobacterial gene expression and regulation, in Molecular Biology of Mycobacteria (McFadden, J., ed.). Surrey University Press, London, UK, pp. 173-198.
20. Harth, G., Lee, B. Y., Wang, J., Clemens, D. L., and Horwitz, M. A. (1996) Novel insights into the genetics, biochemistry, and immunocytochemistry of the 30-kilodalton major extracellular protein of Mycobacterium tuberculosis. Infect.Immunol. 64, 3038-3047.
21. Matsuo, K., Yamaguchi, R., Yamazaki, A., Tasaka, H., and Yamada, T. (1988) Cloning and expression of the Mycobacterium bovis BCG gene for extracellular alpha antigen. J. Bacteriol. 170, 3847-3854.
22. Al-Zarouni, M. and Dale, J. W. (2002) Expression of foreign genes in Mycobacterium bovis BCG strains using different promoters reveals instability of the hsp60 promoter for expression of foreign genes in Mycobacterium bovis BCG strains. Tuberculosis 82, 283-291.
23. Cregg, J. M., Cereghino, J. L., Shi, J., and Higgins, D. R. (2000) Recombinant protein expression in Pichia pastoris. Mol. Biotechnol. 16, 23-52.
24. Cereghino, J. L. and Cregg, J. M. (2000) α mating factor recombinant protein expression in Pichia pastoris. FEMS Micobiol. Rev. 45-66.
25. Romanos, M. A., Scorer, C. A., and Clare, J. J. (1992) Foreign gene expression in yeast. Yeast 8, 423-488.
26. Sreekrishna, K., Brankaamp, R. G., Kropp, K. E., et al. (1997) Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris. Gene 190, 55-62.
27. Wendeler, M., Hoerschemeyer, J., John, M., et al. (2004) Expression of the GM2-activator protein in the methylotrophic yeast Pichia pastoris, purification, isotopic labelling, and biophysical characterization. Prot. Expr. Purif. 34, 147-157.
28. Scorer, C. A., Buckholz, R. G., Clare, J. J., and Romanos, M. A. (1993) The intracellular production and secretion of HIV-1 envelope protein in the methylotrophic yeast Pichia pastoris. Gene 136, 111-119.
29. Macauley-Patrick, S., Fazenda, M. L., McNeil, B., and Harvey, L. M. (2005). Heterologous protein production using the Pichia pastoris expression system. Yeast 22, 249-270.
30. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
31. Guedel-Ben Tanfous, N., Kallel, H., Jarboui, M. A., and Fathallah, D. M. (2005) Expression in Pichia pastoris of a recombinant scFv form of MAb 107, an anti human CD11b integrin antibody. Enzymol. Microb. Technol. 38, 636-642.