Enhanced production of recombinant Mycobacterium tuberculosis antigens in Escherichia coli by replacement of low-usage codons

Infection and Immunity

Volumn 68, Issue 1, 2000, Pages 233-238

  Lakey, David L ^a,b,c   Voladri, Rama K R ^a   Edwards, Kathryn M ^a   Hager, Cynthia ^a   Samten, Buka ^c   Wallis, Robert S ^d   Barnes, Peter F ^c   Kernodle, Douglas S ^a,b  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b VETERANS AFFAIRS MEDICAL CENTER   (United States)

^c UNIVERSITY OF TEXAS HEALTH CENTER   (United States)

^d CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ANTIGEN; MESSENGER RNA; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL GENE; CODON; DNA SEQUENCE; ESCHERICHIA COLI; GENE OVEREXPRESSION; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN PURIFICATION;

EID: 0033970577     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.68.1.233-238.2000     Document Type: Article

References (26)

1. Andersen, P. 1994. Effective vaccination of mice against Mycobacterium tuberculosis infection with a soluble mixture of secreted mycobacterial proteins. Infect. Immun. 62:2536-2544.
2. Atkins, D., H. al-Ghusein, C. Prehaund, and A. R. Coates. 1994. Overproduction and purification of Mycobacterium tuberculosis chaperonin 10. Gene 150:145-148.
3. Bloom, B. R., and P. E. M. Fine. 1994. The BCG experience: implications for the future vaccines against tuberculosis, p. 531-557. In B R. Bloom (ed.), Tuberculosis: pathogenesis, protection, and control. American Society for Microbiology, Washington, D.C.
4. Cannistrara, V. J., M. N. Subbarao, and D. Kennell. 1986. Specific endonucleolytic cleavage sites for decay of Escherichia coli mRNA. J. Mol. Biol. 192:257-274.
5. Cole, S. T., R. Brosch, J. Parkhill, T. Garnier, C. Churcher, D. Harris, S. V. Gordon, K. Eiglmeier, S. Gas, C. E. Barry III, F. Tekaia, K. Badcock, D. Basham, D. Brown, T. Chillingworth, R. Connor, R. Davies, K. Devlin, T. Feltwell, S. Gentles, N. Hamlin, S. Holroyd, T. Hornsby, K. Jagels, A. Krogh, J. McLean, S. Moule, L. Murphy, K. Oliver, J. Osborne, M. A. Quail, M.-A. Rajandream, J. Rogers, S. Rutter, K. Seeger, J. Skelton, R. Squares, S. Squares, J. E. Sulston, K. Taylor, S. Whitehead, and B. G. Barrell. 1998. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393:537-544.
6. Content, J., A. de la Cuvellerie, L. De Wit, V. Vincent-Levy-Frebault, J. Ooms, and J. De Bruyn. 1991. The genes coding for the antigen 85 complexes of Mycobacterium tuberculosis and Mycobacterium bovis BCG are members of a gene family: cloning, sequence determination, and genomic organization of the gene coding for antigen 85-C of M. tuberculosis. Infect. Immun. 59:3205-3212.
7. Dale, J. W., and A. Patki. 1990. Mycobacterial gene expression and regulation, p. 173-198. In J. McFadden (ed.), Molecular biology of the mycobacteria. Surrey University Press, London, United Kingdom.
8. Gallagher, S., S. E. Winston, S. A. Fuller, and J. G. R. Hurrell. 1997. Immunoblotting and immunodetection, p. 10.8,1-10.8,21. In F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.), Current protocols in molecular biology. John Wiley & Sons, Inc., New York, N.Y.
9. Harth, G., B.-Y. Lee, J. Wang, D. L. Clemens, and M. A. Horwitz. 1996. Novel insights into the genetics, biochemistry, and immunocytochemistry of the 30-kilodalton major extracellular protein of Mycobacterium tuberculosis. Infect. Immun. 64:3038-3047.
10. Harth, G., B. Y. Lee, and M. A. Horwitz. 1997. High-level heterologous expression and secretion in rapidly growing nonpathogenic mycobacteria of four major Mycobacterium tuberculosis extracellular proteins considered to be leading vaccine candidates and drug targets. Infect. Immun. 65:2321-2328.
11. Ho, S. N., H. D. Hunt, R. M. Horton, J. K. Pullen, and L. R. Pease. 1989. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77:51-59.
12. Horwitz, M. A., B. W. E. Lee, B. J. Dillion, and G. Harth. 1995. Protection immunity against tuberculosis induced by vaccination with major extracellular proteins of Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. USA 92: 1530-1534.
13. Huygen, K., J. Content, O. Denis, D. L. Montgomery, A. M. Yawman, R. R. Deck, C. M. DeWitt, I. M. Orme, S. Baldwin, C. D'Souza, A. Drowart, E. Lozes, P. Vandendussche, J. P. Van Vooren, M. Liu, and J. B. Ulmer. 1996. Immunogenicity and protective efficacy of a tuberculosis DNA vaccine. Nat. Med. 2:893-898.
14. Makoff, A. J., M. D. Oxer, M. A. Romanos, N. F. Fairweather, and S. Ballantine. 1989. Expression of tetanus toxin fragment C in E. coli: high level expression by removing rare codons. Nucleic Acids Res. 17:10191-10202.
15. Matsuo, K., R. Yamaguchi, A. Yamazaki, H. Tasaka, and T. Yamada. 1988. Cloning and expression of the Mycobacterium bovis BCG gene for extracellular a antigen. J. Bacteriol. 170:3847-3854.
16. Menéndez, M. C., P. Domenech, J. Prieto, and M. J. Garcia. 1995. Cloning and expression of the Mycobacterium fortuitum superoxide dismutase gene. FEMS Microbiol. Lett. 134:273-278.
17. Nehrke, K. W., and T. Platt. 1994. A quaternary transcription termination complex: reciprocal stabilization by Rho factor and NusG protein. J. Mol. Biol. 243:830-893.
18. Pal, P. G., and M. A. Horwitz. 1992. Immunization with extracellular proteins of Mycobacterium tuberculosis induces cell-mediated immune responses and substantial protective immunity in a guinea pig model of pulmonary tuberculosis. Infect. Immun. 60:4781-4792.
19. Platt, T. 1994. Rho and RNA: models for recognition and response. Mol. Microbiol. 11:983-990.
20. Reischl, U., M. Pulz, W. Ehret, and H. Wolf. 1994 PCR-based detection of mycobacteria in sputum samples using a simple and reliable DNA extraction protocol. Biotechniques 17:844-855.
21. Salata, R. A., A. J. Sanson, I. J. Malhotra, H. G. Wiker, M. Harboe, N. B. Phillips, and T. M. Daniel. 1991. Purification and characterization of the 30,000 dalton native antigen of Mycobacterium tuberculosis and characterization of six monoclonal antibodies reactive with a major epitope of this antigen. J. Lab. Clin. Med. 118:589-598.
22. Stanssens, P., E. Remaut, and W. Fiers. 1986. Inefficient translation causes premature transcription termination in the lacZ gene. Cell 44:711-718.
23. Tascon, R. E., M. J. Colston, S. Ragno, E. Stavropoulos, D. Gregory, and D. Lowrie. 1996. Vaccination against tuberculosis by DNA injection. Nat. Med. 2:888-892.
24. Zhang, M., J. Gong, D. V. Iyer, B. E. Jones, R. L. Modlin, and P. F. Barnes. 1994. T cell cytokine responses in persons with tuberculosis and human immunodeficiency virus infection. J. Clin. Invest. 94:2435-2442.
25. Zhang, S., G. Zubay, and E. Goldman. 1991. Low-usage codons in Escherichia coli, yeast, fruit fly and primates. Gene 105:61-72.
26. Zhang, Y., R. Lathigra, T. Garbe, D. Catty, and D. Young. 1991. Genetic analysis of superoxide dismutase, the 23 kilodalton antigen of Mycobacterium tuberculosis. Mol. Microbiol. 5:381-391.