Expression and characterization of Rv2430c, a novel immunodominant antigen of Mycobacterium tuberculosis

Protein Expression and Purification

Volumn 36, Issue 2, 2004, Pages 249-253

  Choudhary, Rakesh K ^a   Pullakhandam, Raghu ^b   Ehtesham, Nasreen Z ^b   Hasnain, Seyed E ^a,c  

^a CENTRE FOR DNA FINGERPRINTING AND DIAGNOSTICS   (India)

^b NATIONAL INSTITUTE OF NUTRITION   (India)

^c JAWAHARLAL NEHRU CENTRE FOR ADVANCED SCIENTIFIC RESEARCH   (India)

Author keywords

Mycobacterium tuberculosis; On column refolding; Rv2430c

Indexed keywords

BACTERIAL PROTEIN; EPITOPE; RV2430C PROTEIN, MYCOBACTERIUM TUBERCULOSIS; UREA;

ARTICLE; CHEMISTRY; CIRCULAR DICHROISM; ESCHERICHIA COLI; GENETICS; IMMUNOLOGY; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; MYCOBACTERIUM TUBERCULOSIS; OPEN READING FRAME; PROTEIN FOLDING; PROTEIN MOTIF; PROTEIN SECONDARY STRUCTURE;

AMINO ACID MOTIFS; BACTERIAL PROTEINS; CHROMATOGRAPHY, LIQUID; CIRCULAR DICHROISM; ESCHERICHIA COLI; IMMUNODOMINANT EPITOPES; MYCOBACTERIUM TUBERCULOSIS; OPEN READING FRAMES; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; UREA;

ESCHERICHIA COLI; MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS;

EID: 3142577686     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.03.016     Document Type: Article

References (21)

1. Cole S.T., et al. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature. 393:1998;537-544
2. Ramakrishnan L., Federspiel N.A., Falkow S. Granuloma-specific expression of Mycobacterium virulence proteins from the glycine-rich PE-PGRS family. Science. 288:2000;1436-1439
3. Banu S., Honore N., Saint-Joanis B., Philpott D., Prevost M.C., Cole S.T. Are the PE-PGRS proteins of Mycobacterium tuberculosis variable surface antigens? Mol. Microbiol. 44:2002;9-19
4. Abou-Zeid C., Garbe T., Lathigra R., Wiker H.G., Harboe M., Rook G.A., Young D.B. Genetic and immunological analysis of Mycobacterium tuberculosis fibronectin-binding proteins. Infect. Immun. 59:1991;2712-2718
5. Delogu G., Brennan M.J. Comparative immune response to PE and PE_PGRSantigens of Mycobacterium tuberculosis. Infect. Immun. 69:2001;5606-5611
6. Espitia C., Laclette J.P., Mondragon-Palomino M., Amador A., Campuzano J., martens A., Singh M., Cicero R., Zhang Y., Moreno C. The PE-PGRS glycine-rich proteins of Mycobacterium tuberculosis: a new family of fibronectin-binding proteins? Microbiology. 145:1999;3487-3495
7. Singh K.K., Zhang X., Patibandla A.S., Chien P. Jr., Laal S. Antigens of Mycobacterium tuberculosis expressed during preclinical tuberculosis: serological immunodominance of proteins with repetitive amino acid sequences. Infect. Immun. 69:2001;4185-4191
8. Brennan M.J., Delogu G., Chen Y., bardarov S., Kriakov M., Alavi M., Jacobs W.R. Jr. Evidence that mycobacterial PE-PGRS proteins are cell surface constituents that influence interactions with other cells. Infect. Immun. 69:2001;7326-7333
9. Sampson S.L., Lukey P., warrem R.M., van Helden P.D., Richardson M., Everett M.J. Expression, characterization and subcellular localization of the Mycobacterium tuberculosis PPE gene Rv1917c. Tuberculosis (Edinb). 81:2001;305-317
10. Ronning D.R., Klabunde T., Besra G.S., Vissa V.D., Belisle J.T., Sacchettini J.C. Crystal structure of the secreted form of antigen 85C reveals potential targets for mycobacterial drugs and vaccines. Nat. Struct. Biol. 7:2000;141-146
11. Anderson D.H., Harth G., Horwitz M.A., Eisenberg D. An interfacial mechanism and a class of inhibitors inferred from two crystal structures of the Mycobacterium tuberculosis 30. kDa major secretory protein (Antigen 85B), a mycolyl transferase J. Mol. Biol. 307:2001;671-681
12. Renshaw P.S., Panagiotidou P., Whelan A., Gordon S.V., Hewinson R.G., Williamson R.A., Carr M.D. Conclusive evidence that the major T-cell antigens of the Mycobacterium tuberculosis complex ESAT-6 and CFP-10 form a tight 1:1 complex and characterisation of the structural properties of ESAT-6, CFP-10 and the ESAT-6.CFP-10 complex. Implications for pathogenesis and virulence. J. Biol. Chem. 277:2002;21598-21603
13. Bloemink M.J., Kemmink J., Dentton E., Muskett F.W., Whelan A., Sheihk A., Hewinson G., Williamson R.A., Carr M.D. Sequence-specific assignment and determination of the secondary structure of the 163 residue M. tuberculosis and M. bovis antigenic protein mpb70. J. Biomol. NMR. 20:2001;185-186
14. Goulding C.W., Parseghian A., Sawaya M.R., Cascio D., Apostol M.I., Gennaro M.L., Eisenberg D. Crystal structure of a major secreted protein of Mycobacterium tuberculosis - MPT63 at 1.5-Å resolution. Protein Sci. 11:2002;2887-2893
15. Devikumar K.R., Kumar K.S., Ramalingam B., Alamelu R. Purification and characterization of three immunodominant proteins (38, 30, and 16. kDa) of Mycobacterium tuberculosis Protein Expr. Purif. 24:2002;188-195
16. Choudhary R.K., Mukhopadhyay S., Chakhaiyar P., Sharma N., Murthy K.J., Katoch V.M., Hasnain S.E. PPE antigen Rv2430c of Mycobacterium tuberculosis induces a strong B-cell response. Infect. Immun. 71:2003;6338-6343
17. Rodriguez G.M., Voskuil M.I., Gold B., Schoolnik G.K., Smith I. IdeR, an essential gene in Mycobacterium tuberculosis: role of IdeR in iron-dependent gene expression, iron metabolism, and oxidative stress response. Infect. Immun. 70:2002;3371-3381
18. Dahl J.L., Kraus C.N., Boshoff H.I., Doan B., Foley K., Avarbock D., Kaplan G., Mizrahi V., Rubin H., Barry III C.E. The role of RelMtb-mediated adaptation to stationary phase in long-term persistence of Mycobacterium tuberculosis in mice. Proc. Natl. Acad. Sci. USA. 100:2003;10026-10031
19. Boeggeman E.E., Ramakrishnan B., Qasba P.K. The N-terminal stem region of bovine and human beta1, 4-galactosyltransferase I increases the in vitro folding efficiency of their catalytic domain from inclusion bodies. Protein Expr. Purif. 30:2003;219-229
20. Srinivas V., Raman B., Rao K.S., Ramakrishna T., Rao Ch.M. Structural perturbation and enhancement of the chaperone-like activity of alpha-crystallin by arginine hydrochloride. Protein Sci. 12:2003;1262-1270
21. Pullakhandham R., Ghosh S., Soundarya K., Haseeb A., Aruna B., Ehtesham N.Z. Dimerization of human recombinant resistin involves covalent and non-covalent interactions. Biochem. Biophys. Res. Commun. 313:2004;652-656