Reduced susceptibility to polyenes associated with a missense mutation in the ERG6 gene in a clinical isolate of Candida glabrata with pseudohyphal growth

Antimicrobial Agents and Chemotherapy

Volumn 51, Issue 3, 2007, Pages 982-990

  Vandeputte, Patrick ^a   Tronchin, Guy ^a   Bergès, Thierry ^b   Hennequin, Christophe ^c   Chabasse, Dominique ^a   Bouchara, Jean Philippe ^a  

^a ANGERS UNIVERSITY HOSPITAL   (France)

^b UNIVERSITÉ DE POITIERS   (France)

^c HÔPITAL TENON   (France)

Author keywords

[No Author keywords available]

Indexed keywords

AMPHOTERICIN B; CASPOFUNGIN; CLOTRIMAZOLE; CYSTEINE; ERGOSTEROL; FLUCONAZOLE; ITRACONAZOLE; KETOCONAZOLE; MICONAZOLE; NYSTATIN; PHENYLALANINE; POLYENE; STEROL DERIVATIVE; VORICONAZOLE;

AMINO ACID SUBSTITUTION; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; CANDIDA GLABRATA; CELL GROWTH; CELL MEMBRANE; CELL WALL; CGERG11 GENE; CGERG4 GENE; CGERG5 GENE; CONIDIUM; CULTURE MEDIUM; DIFFUSION; ERG6 GENE; FUNGUS HYPHAE; FUNGUS ISOLATION; GENE; GENE MUTATION; GENE OVEREXPRESSION; GENE SEQUENCE; GENETIC CODE; GENETIC COMPLEMENTATION; HUMAN; MINIMUM INHIBITORY CONCENTRATION; MISSENSE MUTATION; MOLECULAR DYNAMICS; MORPHOLOGY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; ULTRASTRUCTURE; WILD TYPE;

ANTIFUNGAL AGENTS; CANDIDA GLABRATA; CANDIDIASIS; DNA PRIMERS; GENES, FUNGAL; GENETIC COMPLEMENTATION TEST; METHYLTRANSFERASES; MICROBIAL SENSITIVITY TESTS; MOLECULAR SEQUENCE DATA; MUTATION, MISSENSE; PHENOTYPE; POLYENES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, FUNGAL; RNA, MESSENGER; STEROLS;

EID: 33847611100     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/AAC.01510-06     Document Type: Article

References (42)

1. Arthington, B. A., L. G. Bennett, P. L. Skatrud, C. J. Guynn, R. J. Barbuch, C. E. Ulbright, and M. Bard. 1991. Cloning, disruption and sequence of the gene encoding yeast C-5 sterol desaturase. Gene 102:39-44.
2. Ashman, W. H., R. J. Barbuch, C. E. Ulbright, H. W. Jarrett, and M. Bard. 1991. Cloning and disruption of the yeast C-8 sterol isomerase gene. Lipids 26:628-632.
3. Barker, K. S., S. Crisp, N. Wiederhold, R. E. Lewis, B. Bareither, J. Eckstein, R. Barbuch, M. Bard, and P. D. Rogers. 2004. Genome-wide expression profiling reveals genes associated with amphotericin B and fluconazole resistance in experimentally induced antifungal resistant isolates of Candida albicans. J. Antimicrob. Chemother. 54:376-385.
4. Brun, S., C. Aubry, O. Lima, R. Filmon, T. Bergès, D. Chabasse, and J. P. Bouchara. 2003. Relationships between respiration and susceptibility to azole antifungals in Candida glabrata. Antimicrob. Agents Chemother. 47:847-853.
5. Brun, S., T. Bergès, P. Poupard, C. Vauzelle-Moreau, G. Renier, D. Chabasse, and J. P. Bouchara. 2004. Mechanisms of azole resistance in petite mutants of Candida glabrata. Antimicrob. Agents Chemother. 48:1788-1796.
6. Calcagno, A. M., E. Bignell, P. Warn, M. D. Jones, D. W. Denning, F. A. Muhlschlegel, T. R. Rogers, and K. Haynes. 2003. Candida glabrata STE12 is required for wild-type levels of virulence and nitrogen starvation induced filamentation. Mol. Microbiol. 50:1309-1318.
7. Casey, W. M., J. P. Burgess, and L. W. Parks. 1991. Effect of sterol side-chain structure on the feed-back control of sterol biosynthesis in yeast. Biochim. Biophys. Acta 1081:279-284.
8. Csank, C., and K. Haynes. 2000. Candida glabrata displays pseudohyphal growth. FEMS Microbiol. Lett. 189:115-120.
9. Defontaine, A., J. P. Bouchara, P. Declerk, C. Planchenault, D. Chabasse, and J. N. Hallet. 1999. In-vitro resistance to azoles associated with mitochondrial DNA deficiency in Candida glabrata. J. Med. Microbiol. 48:663-670.
10. Ellis, D. 2002. Amphotericin B: spectrum and resistance. J. Antimicrob. Chemother. 49(Suppl. 1):7-10.
11. Gaber, R. F., D. M. Copple, B. K. Kennedy, M. Vidal, and M. Bard. 1989. The yeast gene ERG6 is required for normal membrane function but is not essential for biosynthesis of the cell-cycle-sparking sterol. Mol. Cell. Biol. 9:3447-3456.
12. Graham, T. R., P. A. Scott, and S. D. Emr. 1993. Brefeldin A reversibly blocks early but not late protein transport steps in the yeast secretory pathway. EMBO J. 12:869-877.
13. Hemmi, K., C. Julmanop, D. Hirata, E. Tsuchiya, J. Y. Takemoto, and T. Miyakawa. 1995. The physiological roles of membrane ergosterol as revealed by the phenotypes of syr1/erg3 null mutant of Saccharomyces cerevisiae. Biosci. Biotechnol. Biochem. 3:482-486.
14. Jensen-Pergakes, K. L., M. A. Kennedy, N. D. Lees, R. Barbuch, C. Koegel, and M. Bard. 1998. Sequencing, disruption, and characterization of the Candida albicans sterol methyltransferase (ERG6) gene: drug susceptibility studies in erg6 mutants. Antimicrob. Agents Chemother. 42:1160-1167.
15. Kelly, S. L., D. C. Lamb, D. E. Kelly, N. J. Manning, J. Loeffler, H. Hebart, U. Schumacher, and H. Einsele. 1997. Resistance to fluconazole and cross-resistance to amphotericin B in Candida albicans from AIDS patients caused by defective delat 5,6-desaturation. FEBS Lett. 400:80-82.
16. Krcmery, V., and A. J. Barnes. 2002. Non-albicans Candida spp. causing fungaemia: pathogenicity and antifungal resistance. J. Hosp. Infect. 50:243-260.
17. Lachke, S. A., S. Joly, K. Daniels, and D. R. Soll. 2002. Phenotypic switching and filamentation in Candida glabrata. Microbiology 148:2661-7264.
18. Lees, N. D., B. Skaggs, D. R. Kirsch, and M. Bard. 1995. Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae-a review. Lipids 30:221-2266.
19. Lorenz, R. T., and L. W. Parks. 1992. Cloning, sequencing, and disruption of the gene encoding sterol C-14 reductase in Saccharomyces cerevisiae. DNA Cell Biol. 11:685-692.
20. Niimi, M., K. Niimi, Y. Takano, A. R. Holmes, F. J. Fischer, Y. Uehara, and R. D. Cannon. 2004. Regulated overexpression of CDR1 in Candida albicans confers multidrug resistance. J. Antimicrob. Chemother. 6:999-1006.
21. Nissapatorn, V., C. Lee, Q. K. Fatt, and K. A. Abdullah. 2003. AIDS-related opportunistic infections in Hospital Kuala Lumpur. Jpn. J. Infect. Dis. 56:187-192.
22. Nolte, F. S., T. Parkinson, D. J. Falconer, S. Dix, J. Williams, C. Gilmore, R. Geller, and J. R. Wingard. 1997. Isolation and characterization of fluconazole- and amphotericin B-resistant Candida albicans from blood of two patients with leukemia. Antimicrob. Agents Chemother. 41:196-199.
23. Odds, F. C., M. G. Rinaldi, C. R. Cooper, Jr., A. Fothergill, L. Pasarell, and M. R. McGinnis. 1997. Candida and Torulopsis: a blinded evaluation of use of pseudohypha formation as basis for identification of medically important yeasts. J. Clin. Microbiol. 35:313-316.
24. Paltauf, F., S. Kohlwein, and S. A. Henry. 1992. Regulation and compartmentalization of lipid synthesis in yeast, p. 415-500. In E. W. Jones, J. R. Pringle, and J. R. Broach (ed.), The molecular and cellular biology of the yeast Saccharomyces: gene expression. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
25. Parks, L. W., and W. M. Casey. 1995. Physiological implications of sterol biosynthesis in yeast. Annu. Rev. Microbiol. 49:95-116.
26. Perea, S., and T. F. Patterson. 2002. Antifungal resistance in pathogenic fungi. Clin. Infect. Dis. 35:1073-1080.
27. Prendergast, J. A., R. A. Singer, N. Rowley, A. Rowley, G. C. Johnston, M. Danos, B. Kennedy, and R. F. Gaber. 1995. Mutations sensitizing yeast cells to the start inhibitor nalidixic acid. Yeast 6:537-547.
28. Sanglard, D., F. Ischer, T. Parkinson, D. Falconer, and J. Bille. 2003. Candida albicans mutations in the ergosterol biosynthetic pathway and resistance to several antifungal agents. Antimicrob. Agents Chemother. 47:2404-2412.
29. Sanglard, D., and F. C. Odds. 2002. Resistance of Candida species to antifungal agents: molecular mechanisms and clinical consequences. Lancet Infect. Dis. 2:73-85.
30. Servouse, M., and F. Karst. 1986. Regulation of early enzymes of ergosterol biosynthesis in Saccharomyces cerevisiae. Biochem. J. 240:541-547.
31. Singh, A., I. Bairy, and P. G. Shivananda. 2003. Spectrum of opportunistic infections in AIDS cases. Indian J. Med. Sci. 57:16-21.
32. Smith, S. J., and L. W. Parks. 1993. The ERG3 gene in Saccharomyces cerevisiae is required for the utilization of respiratory substrates and in heme-deficient cells. Yeast 11:1177-1187.
33. Smith, S. J., J. H. Crowley, and L. W. Parks. 1996. Transcriptional regulation by ergosterol in the yeast Saccharomyces cerevisiae. Mol. Cell Biol. 16:5427-5432.
34. Taguchi, N., Y. Takano, C. Julmanop, Y. Wang, S. Stock, J. Takemoto, and T. Miyakawa. 1994. Identification and analysis of the Saccharomyces cerevisiae SYR1 gene reveals that ergosterol is involved in the action of syringomycin. Microbiology 2:353-359.
35. Tronchin, G., J. P. Bouchara, R. Robert, and J. M. Senet. 1988. Adherence of Candida albicans germ tubes to plastic: ultrastructural and molecular studies of fibrillar adhesins. Infect. Immun. 56:1987-1993.
36. Tsai, H. F., M. Bard, K. Izumikawa, A. A. Krol, A. M. Sturm, N. T. Culbertson, C. A. Pierson, and J. E. Bennett. 2004. Candida glabrata erg1 mutant with increased sensitivity to azoles and to low oxygen tension. Antimicrob. Agents Chemother. 48:2483-2489.
37. Umebayashi, K., and A. Nakano. 2003. Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J. Cell Biol. 161:1117-1131.
38. Vandeputte, P., G. Larcher, T. Bergès, G. Renier, D. Chabasse, and J. P. Bouchara. 2005. Mechanisms of azole resistance in a clinical isolate of Candida tropicalis. Antimicrob. Agents Chemother. 11:4608-4615.
39. Vermitsky, J. P., K. D. Earhart, W. L. Smith, R. Homayouni, T. D. Edlind, and P. D. Rogers. 2006. Pdr1 regulates multidrug resistance in Candida glabrata: gene disruption and genome-wide expression studies. Mol. Microbiol. 61:704-722.
40. Watson, P. F., M. E. Rose, and S. L. Kelly. 1998. Isolation and analysis of ketoconazole resistant mutants of Saccharomyces cerevisiae. J. Med. Vet. Mycol. 3:153-162.
41. White, T. C., K. A. Marr, and R. A. Bowden. 1998. Clinical, cellular, and molecular factors that contribute to antifungal drug resistance. Clin. Microbiol. Rev. 11:382-402.
42. Young, L. Y., C. M. Hull, and J. Heitman. 2003. Disruption of ergosterol biosynthesis confers resistance to amphotericin B in Candida lusitaniae. Antimicrob. Agents Chemother. 47:2717-2724.