Candida glabrata erg1 mutant with increased sensitivity to azoles and to low oxygen tension

Antimicrobial Agents and Chemotherapy

Volumn 48, Issue 7, 2004, Pages 2483-2489

  Tsai, Huei Fung ^a   Bard, Martin ^b   Izumikawa, Koichi ^a   Krol, Anna A ^a   Sturm, Aaron M ^b   Culbertson, Nicholas T ^b   Pierson, Charles A ^b   Bennett, John E ^a,c  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^b INDIANA UNIVERSITY   (United States)

^c NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; CHOLESTEROL; FLUCONAZOLE; ITRACONAZOLE; PYRROLE DERIVATIVE; RHODAMINE 6G; SQUALENE; SQUALENE MONOOXYGENASE; STEROL; TERBINAFINE;

AMINO ACID SEQUENCE; ARTICLE; CANDIDA GLABRATA; CGERG1 GENE; CODON; CONTROLLED STUDY; DRUG RESISTANCE; DRUG SENSITIVITY; DRUG UPTAKE; FUNGAL GENE; FUNGUS GROWTH; FUNGUS MUTANT; GENE IDENTIFICATION; GENE MUTATION; GENETIC CODE; GENETIC COMPLEMENTATION; LIPID COMPOSITION; MUTAGENESIS; NONHUMAN; OXYGEN TENSION; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; STEROL SYNTHESIS; TRANSPOSON; WILD TYPE;

ANTIFUNGAL AGENTS; AZOLES; CANDIDA GLABRATA; CHOLESTEROL; CLONING, MOLECULAR; DNA TRANSPOSABLE ELEMENTS; DRUG RESISTANCE, FUNGAL; FLUCONAZOLE; FLUORESCENT DYES; GENETIC COMPLEMENTATION TEST; MICROBIAL SENSITIVITY TESTS; MUTATION; OXYGEN; PHENOTYPE; PLASMIDS; POTASSIUM CHANNELS; POTASSIUM CHANNELS, VOLTAGE-GATED; RHODAMINES; SQUALENE; STEROLS;

EID: 3042531451     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/AAC.48.7.2483-2489.2004     Document Type: Article

References (20)

1. Bennett, J. E., K. Izumikawa, and K. A. Marr. 2004. Mechanism of increased fluconazole resistance in Candida glabrata during prophylaxis. Antimicrob. Agents Chemother. 48:1773-1777.
2. Botstein, D., S. C. Falco, S. E. Stewart, M. Brennan, S. Scherer, D. T. Stinchcomb, K. Struhl, and R. W. Davis. 1979. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8:17-24.
3. Castano, I., R. Kaur, S. Pan, R. Cregg, L. Penas Ade, N. Guo, M. C. Biery, N. L. Craig, and B. P. Cormack. 2003. Tn7-based genome-wide random insertional mutagenesis of Candida glabrata. Genome Res. 13:905-915.
4. Fonzi, W. A., and M. Y. Irwin. 1993. Isogenic strain construction and gene mapping in Candida albicans. Genetics 134:717-728.
5. Geber, A., C. A. Hitchcock, J. E. Swartz, F. S. Pullen, K. E. Marsden, K. J. Kwon-Chung, and J. E. Bennett. 1995. Deletion of the Candida glabrata ERG3 and ERG11 genes: effect on cell viability, cell growth, sterol composition, and antifungal susceptibility. Antimicrob. Agents Chemother. 39:2708-2717.
6. Hongay, C., N. Jia, M. Bard, and F. Winston. 2002. Mot3 is a transcriptional repressor of ergosterol biosynthetic genes and is required for normal vacuolar function in Saccharomyces cerevisiae. EMBO J. 21:4114-4124.
7. Izumikawa, K., H. Kakeya, H. F. Tsai, B. Grimberg, and J. E. Bennett. 2003. Function of Candida glabrata ABC transporter gene, PDH1. Yeast 20:249-261.
8. Kitada, K., E. Yamaguchi, and M. Arisawa. 1996. Isolation of a Candida glabraia centromere and its use in construction of plasmid vectors. Gene 175:105-108.
9. Klobucnikova, V., P. Kohut, R. Leber, S. Fuchsbichler, N. Schweighofer, F. Turnowsky, and I. Hapala. 2003. Terbinafine resistance in a pleiotropic yeast mutant is caused by a single point mutation in the ERG1 gene. Biochem. Biophys. Res. Commun. 309:666-671.
10. Landl, K. M., B. Klosch, and F. Turnowsky. 1996. ERG1, encoding squalene epoxidase, is located on the right arm of chromosome VII of Saccharomyces cerevisiae. Yeast 12:609-613.
11. Leber, R., S. Fuchsbichler, V. V. Klobucnikova, N. Schweighofer, E. Pitters, K. Wohlfarter, M. Lederer, K. Landl, C. Ruckenstuhl, I. Hapala, and F. Turnowsky. 2003. Molecular mechanism of terbinafine resistance in Saccharomyces cerevisiae. Antimicrob. Agents Chemother. 47:3890-3900.
12. Maesaki, S., P. Marichal, H. Vanden Bossche, D. Sanglard, and S. Kohno. 1999. Rhodamine 6G efflux for the detection of CDR1-overexpressing azole-resistant Candida albicans strains. J. Antimicrob. Chemother. 44:27-31.
13. Molzahn, S. W., and R. A. Woods. 1972. Polyene resistance and the isolation of sterol mutants in Saccharomyces cerevisiae. J. Gen. Microbiol. 72:339-348.
14. Nakayama, H., M. Izuta, N. Nakayama, M. Arisawa, and Y. Aoki. 2000. Depletion of the squalene synthase (ERG9) gene does not impair growth of Candida glabrata in mice. Antimicrob. Agents Chemother. 44:2411-2418.
15. Nakayama, H., N. Nakayama, M. Arisawa, and Y. Aoki. 2001. In vitro and in vivo effects of 14-α-demethylase (ERG11) depletion in Candida glabrata. Antimicrob. Agents Chemother. 45:3037-3045.
16. National Committee for Clinical Laboratory Standards. 1995. Reference method for broth dilution antifungal susceptibility testing of yeast. Approved standard M27-A. National Committee for Clinical Laboratory Standards, Wayne, Pa.
17. Parkinson, T., D. J. Falconer, and C. A. Hitchcock. 1995. Fluconazole resistance due to energy-dependent drug efflux in Candida glabrata. Antimicrob. Agents Chemother. 39:1696-1699.
18. Pfaller, M. A., S. A. Messer, R. J. Hollis, R. N. Jones, and D. J. Diekema. 2002. In vitro activities of ravuconazole and voriconazole compared with those of four approved systemic antifungal agents against 6,970 clinical isolates of Candida spp. Antimicrob. Agents Chemother. 46:1723-1727.
19. Sambrook, J., and W. Russell (ed.). 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
20. Wilson, R. B., D. Davis, and A. P. Mitchell. 1999. Rapid hypothesis testing with Candida albicans through gene disruption with short homology regions. J. Bacteriol. 181:1868-1874.