Transformation by RAS oncogene decreases the width of substrate-spread fibroblasts but not their length

Cell Biology International

Volumn 31, Issue 3, 2007, Pages 220-223

  Kharitonova, Margarita A ^a   Kopnin, Pavel B ^b,c   Vasiliev, Jury M ^a  

^a N N BLOKHIN RUSSIAN CANCER RESEARCH CENTER   (Russian Federation)

^b ENGELHARDT INSTITUTE OF MOLECULAR BIOLOGY   (Russian Federation)

^c UNIVERSITY OF OSLO   (Norway)

Author keywords

Cell shape; Fibroblast; Morphometry; RAS oncogene; Transformation

Indexed keywords

ANIMAL CELL; ARTICLE; BLAST TRANSFORMATION; CELL POLARITY; CELL SHAPE; CELL SPREADING; CELL STRUCTURE; CELL TRANSFORMATION; CONTROLLED STUDY; EMBRYO; FIBROBLAST; GENE EXPRESSION; MOUSE; NONHUMAN; ONCOGENE RAS; RAT;

RATTUS;

EID: 33847074143     PISSN: 10656995     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cellbi.2006.10.008     Document Type: Article

References (23)

1. Alexandrova A.Y., Dugina V.B., Paterson H., Bershadsky A.D., and Vasiliev J.M. Motility of intracellular particles in rat fibroblasts is greatly enhanced by phorbol ester and by over-expression of normal p21N-ras. Cell Motil Cytoskeleton 25 (1993) 254-266
2. Clark E.A., Golub T.R., Lander E.S., and Hynes R.O. Genomic analysis of metastasis reveals an essential role for RhoC. Nature 406 (2000) 532-535
3. Dunn G.A., and Brown A.F. Alignment of fibroblasts on grooved surfaces described by a simple geometric transformation. J Cell Sci 83 (1986) 313-340
4. Fritz G., Just I., and Kaina B. Rho GTPases are over-expressed in human tumors. Int J Cancer 81 (1999) 682-687
5. Harvey D.M., and Levine A.J. p53 alteration is a common event in the spontaneous immortalization of primary BALB/c murine embryo fibroblasts. Genes Dev 5 (1991) 2375-2385
6. Heckman C.A., and Jamasbi R.J. Describing shape dynamics in transformed cells through latent factors. Exp Cell Res 246 (1999) 69-82
7. Lennette D.A. An improved mounting medium for immunofluorescence microscopy. Am J Clin Pathol 69 (1978) 647-648
8. Levina E.M., Kharitonova M.A., Rovensky Y.A., and Vasiliev J.M. Cytoskeletal control of fibroblast length: experiments with linear strips of substrate. J Cell Sci 114 (2001) 4335-4341
9. Lin A.W., Barradas M., Stone J.C., van Aelst L., Serrano M., and Lowe S.W. Premature senescence involving p53 and p16 is activated in response to constitutive MEK/MAPK mitogenic signaling. Genes Dev 12 (1998) 3008-3019
10. Lombardi L., Ballinari D., Bongarzone I., Migliari M., Mondellini P., Traversari C., et al. Ultrastructural cytoskeleton alterations and modification of actin expression in the NIH/3T3 cell line after transformation with Ha-ras-activated oncogene. Cell Motil Cytoskeleton 15 (1990) 220-229
11. Nobes C.D., and Hall A. Rho GTPases control polarity, protrusion, and adhesion during cell movement. J Cell Biol 144 (1999) 1235-1244
12. Omelchenko T., Vasiliev J.M., Gelfand I.M., Feder H.H., and Bonder E.M. Mechanisms of polarization of the shape of fibroblasts and epitheliocytes: separation of the roles of microtubules and Rho-dependent actin-myosin contractility. Proc Natl Acad Sci U S A 99 (2002) 10452-10457
13. Pollack R., Osborn M., and Weber K. Patterns of organization of actin and myosin in normal and transformed cultured cells. Proc Natl Acad Sci U S A 72 (1975) 994-998
14. Sahai E., and Marshall C.J. RHO-GTPases and cancer. Nat Rev Cancer 2 (2002) 133-142
15. Sahai E., Olson M.F., and Marshall C.J. Cross-talk between Ras and Rho signalling pathways in transformation favours proliferation and increased motility. EMBO J 20 (2001) 755-766
16. Serrano M., Lee H., Chin L., Cordon-Cardo C., Beach D., and DePinho R.A. Role of the INK4a locus in tumor suppression and cell mortality. Cell 85 (1996) 27-37
17. Suwa H., Ohshio G., Imamura T., Watanabe G., Arii S., Imamura M., et al. Overexpression of the rhoC gene correlates with progression of ductal adenocarcinoma of the pancreas. Br J Cancer 77 (1998) 147-152
18. Topp W.C. Normal rat cell lines deficient in nuclear thymidine kinase. Virology 113 (1981) 408-411
19. Turovets N.A., Chumakov P.M., and Kopnin B.P. Effect of inactivating various components of the signal pathways of the tumor suppressor p53 on genomic stability. Genetika 35 (1999) 1651-1658
20. Vasiliev J.M. Spreading of non-transformed and transformed cells. Biochim Biophys Acta 780 (1985) 21-65
21. Waterman-Storer C.M., Worthylake R.A., Liu B.P., Burridge K., and Salmon E.D. Microtubule growth activates Rac1 to promote lamellipodial protrusion in fibroblasts. Nat Cell Biol 1 (1999) 45-50
22. Wilkinson S., Paterson H.F., and Marshall C.J. Cdc42-MRCK and Rho-ROCK signalling cooperate in myosin phosphorylation and cell invasion. Nat Cell Biol 7 (2005) 255-261
23. Worthylake R.A., Lemoine S., Watson J.M., and Burridge K. RhoA is required for monocyte tail retraction during transendothelial migration. J Cell Biol 154 (2001) 147-160