On the activity loss of hydrolases in organic solvents: II. A mechanistic study of subtilisin Carlsberg

BMC Biotechnology

Volumn 6, Issue , 2006, Pages

  Castillo, Betzaida ^a   Bansal, Vibha ^b   Ganesan, Ashok ^c   Halling, Peter ^c   Secundo, Francesco ^d   Ferrer, Amaris ^b   Griebenow, Kai ^a   Barletta, Gabriel ^b  

^a UNIVERSITY OF PUERTO RICO   (Puerto Rico)

^b UNIVERSITY OF PUERTO RICO   (Puerto Rico)

^c UNIVERSITY OF STRATHCLYDE   (United Kingdom)

^d CNR   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

COLYOPHILIZATION; ENZYME STORAGE STABILITY; HYDROLASES; LYOPHILIZATION; STRUCTURAL PERTURBATIONS; SUBTILISIN CARLSBERG;

AGGLOMERATION; CATALYSIS; DEHYDRATION; IONIZATION; ORGANIC SOLVENTS; PARAMAGNETIC RESONANCE; PH; TITRATION;

ENZYME KINETICS;

ALKALINE PROTEINASE; BARIUM DERIVATIVE; DIOXANE; HYDROLASE; METHYL BETA CYCLODEXTRIN; NITROXIDE; ORGANIC SOLVENT; SUBTILISIN; WATER;

ARTICLE; CONTROLLED STUDY; ELECTRON SPIN RESONANCE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STABILITY; ENZYME STRUCTURE; FREEZE DRYING; HYDRATION; INCUBATION TIME; IONIZATION; MICHAELIS CONSTANT; PH; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE;

BETA-CYCLODEXTRINS; BINDING SITES; CHEMISTRY, ORGANIC; CIRCULAR DICHROISM; ELECTRON SPIN RESONANCE SPECTROSCOPY; HYDROGEN-ION CONCENTRATION; HYDROLASES; KINETICS; MODELS, CHEMICAL; SOLVENTS; SUBTILISINS; WATER;

EID: 33846595722     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-6-51     Document Type: Article

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