Active-site titration of serine proteases in organic solvents

Biotechnology and Bioengineering

Volumn 50, Issue 3, 1996, Pages 329-335

  Wangikar, Pramod P ^a   Carmichael, Douglas ^a   Clark, Douglas S ^b   Dordick, Jonathan S ^a  

^a IA United States   (United States)

^b University of California   (United States)

Author keywords

active site titration; organic solvents; serine proteases

Indexed keywords

AROMATIC COMPOUNDS; CALCULATIONS; CATALYSIS; ENZYME INHIBITION; ENZYMES; HYDRATION; ORGANIC SOLVENTS; REACTION KINETICS;

ACTIVE SITE TITRATION; CHYMOTRYPSIN; HYDROPHOBICITY; PHENYL METHANESULFONYL FLUORIDE; SERINE PROTEASES; SUBTILISIN; TETRAHYDROFURAN;

TITRATION;

ORGANIC SOLVENT; SERINE PROTEINASE;

ARTICLE; DEACYLATION; ENZYME ACTIVE SITE; ENZYME CONFORMATION; ENZYME INHIBITION; ENZYME KINETICS; ENZYME MECHANISM; GEL PERMEATION CHROMATOGRAPHY; HYDROPHOBICITY; NONHUMAN; STOICHIOMETRY;

EID: 0030570129     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19960505)50:3<329::AID-BIT11>3.0.CO;2-I     Document Type: Article

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