Cryopreservation of primary human hepatocytes: The benefit of trehalose as an additional cryoprotective agent

Liver Transplantation

Volumn 13, Issue 1, 2007, Pages 38-45

  Katenz, Ekaterina ^a   Vondran, Florian Wolfgang Rudolf ^a   Schwartlander, Ruth ^a   Pless, Gesine ^a   Gong, Xiaobing ^a   Cheng, Xiandong ^a   Neuhaus, Peter ^a   Sauer, Igor Maximilian ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ALBUMIN; ASPARTATE AMINOTRANSFERASE; CRYOPROTECTIVE AGENT; DIMETHYL SULFOXIDE; TREHALOSE; UREA;

ARTICLE; CELL ADHESION; CELL ISOLATION; CELL METABOLISM; CELL PROLIFERATION; CELL VIABILITY; CLINICAL ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; CRYOPRESERVATION; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME RELEASE; FREEZING; HUMAN; HUMAN CELL; HUMAN TISSUE; LIVER CELL; LIVER RESECTION; LIVER TUMOR; PILOT STUDY; PLATING MEDIUM; PRIORITY JOURNAL; THAWING;

ALBUMINS; ANIMALS; ASPARTATE AMINOTRANSFERASES; CELL PROLIFERATION; CELL SURVIVAL; CRYOPRESERVATION; CRYOPROTECTIVE AGENTS; DIMETHYL SULFOXIDE; HEPATOCYTES; HUMANS; L-LACTATE DEHYDROGENASE; LIVER; PILOT PROJECTS; RATS; TREHALOSE;

EID: 33846249979     PISSN: 15276465     EISSN: 15276473     Source Type: Journal    
DOI: 10.1002/lt.20921     Document Type: Article

References (32)

1. Hughes RD, Mitry RR, Dhawan A. Hepatocyte transplantation for metabolic liver disease: UK experience. J R Soc Med 2005;98:341-345.
2. Yuan J, Liu L, Shimada M, Wang A, Ruhnke M, Heeckt P, et al. Induction, expression and maintenance of cytochrome P450 isoforms in long-term cultures of primary human hepatocytes. ALTEX 2004;21:3-11.
3. Hengstler JG, Utesch D, Steinberg P, Platt KL, Diener B, Ringel M, et al. Cryopreserved primary hepatocytes as a constantly available in vitro model for the evaluation of human and animal drug metabolism and enzyme induction. Drug Metab Rev 2000;32:81-118.
4. Alexandre E, Viollon-Abadie C, David P, Gandillet A, Coassolo P, Heyd B, et al. Cryopreservation of adult human hepatocytes obtained from resected liver biopsies. Cryobiology 2002;44:103-113.
5. Chesne C, Guyomard C, Fautrel A, Poullain MG, Fremond B, De Jong H, et al. Viability and function in primary culture of adult hepatocytes from various animal species and human beings after cryopreservation. Hepatology 1993;18:406-414.
6. Lloyd TD, Orr S, Skett P, Berry DP, Dennison AR. Cryopreservation of hepatocytes: a review of current methods for banking. Cell Tissue Bank 2003;4:3-15.
7. Lovelock JE, Bishop MW. Prevention of freezing damage to living cells by dimethyl sulphoxide. Nature 1959; 183: 1394-1395.
8. Gutteridge JM, Quinlan GJ. Antioxidant protection against organic and inorganic oxygen radicals by normal human plasma: the important primary role for iron-binding and iron-oxidizing proteins. Biochim Biophys Acta 1993;1156:144-150.
9. Limaye LS, Kale VP. Cryopreservation of human hematopotetic cells with membrane stabilizers and bioantioxidants as additives in the conventional freezing medium. J Hematother Stem Cell Res 2001;10:709-718.
10. Dorko K, Freeswick PD, Bartoli F, Cicalese L, Bardsley BA, Tzakis A, et al. A new technique for isolating and culturing human hepatocytes from whole or split livers not used for transplantation. Cell Transplant 1994;3:387-395.
11. Li AP, Gorycki PD, Hengstler JG, Kedderis GL, Koebe HG, Rahmani R, et al. Present status of the application of cryopreserved hepatocytes in the evaluation of xenobiotics: consensus of an international expert panel. Chem Biol Interact 1999;121:117-123.
12. Yu ZW, Quinn PJ. Dimethyl sulphoxide: a review of its applications in cell biology. Biosci Rep 1994;14:259-281.
13. Sasnoor LM, Kale VP, Limaye IS. Supplementation of conventional freezing medium with a combination of catalase and trehalose results in better protection of surface molecules and functionality of hematopoietic cells. J Hematother Stem Cell Res 2003;12:553-564.
14. Sztein JM, Noble K, Farley JS, Mobraaten LE. Comparison of permeating and nonpermeating cryoprotectants for mouse sperm cryopreservation. Cryobiology 2001;42:28-39.
15. Byrd W. Cryopreservation, thawing, and transfer of human embryos. Semin Reprod Med 2002;20:37-43.
16. Colaco C, Kampinga J, Roser B. Amorphous stability and trehalose. Science 1995;268:788.
17. Sun WQ, Davidson P. Protein inactivation in amorphous sucrose and trehalose matrices: effects of phase separation and crystallization. Biochim Biophys Acta 1998;1425:235-244.
18. Crowe LM, Reid DS, Crowe JH. Is trehalose special for preserving dry biomaterials? Biophys J 1996:71:2087-2093.
19. Crowe LM, Mouradian R, Crowe JH, Jackson SA, Womersley C. Effects of carbohydrates on membrane stability at low water activities. Biochim Blophys Acta 1984;769:141-150.
20. Richards AB, Krakowka S, Dester LB, Schmid H, Wolterbeek APM, Waalkens-Berendsen DH, et al. Trehalose: a review of properties, history of use and human tolerance, and results of multiple safety studies. Food Chem Toxicol 2002;40:871-898.
21. Patist A, Zoerb H. Preservation mechanisms of trehalose in food and biosystems. Colloids Surf B Biointerfaces 2005;40:107-113.
22. Arikura J, Kobayashi N, Okitsu T, Noguchi H, Totsugawa T, Watanabe T, et al. UW solution: a promising tool for cryopreservation of primarily isolated rat hepatocytes. J Hepatobiliary Pancreat Surg 2002;9:742-749.
23. Kuniedas T, Maruyama M, Okitsu T, Shibata N, Takesue M, Totsugawa T, et al. Cryopreservation of primarily isolated porcine hepatocytes with UW solution. Cell Transplant 2003;12:607-616.
24. Eroglu A, Russo MJ, Bieganski R, Fowler A, Cheley S, Bayley H, et al. Intracellular trehalose improves the survival of cryopreserved mammalian cells. Nat Biotechnol 2000;18:163-167.
25. Beattie GM, Crowe JH, Lopez AD, Cirulli V, Ricordi C, Hayek A. Trehalose: a cryoprotectant that enhances recovery and preserves function of human pancreatic islets after long-term storage. Diabetes 1997;46:519-523.
26. Ostrowska A, Bode DC, Pruss J, Bilir B, Smith GD, Zeisloft S. Investigation of functional and morphological integrity of freshly isolated and cryopreserved human hepatocytes. Cell Tissue Bank 2000;1:55-68.
27. Ji L, de Pablo JJ, Palecek SP. Cryopreservation of adherent human embryonic stem cells. Biotechnol Bioeng 2004; 88:299-312.
28. Nie Y, de Pablo JJ, Palecek SP. Platelet cryopreservation using a trehalose and phosphate formulation. Biotechnol Bioeng 2005;92:79-90.
29. Bischof JC, Wokers WF, Tsvetkova NM, Oliver AE, Crowe JH. Lipid and protein changes due to freezing in Dunning AT-1 cells. Cryobiology 2002;45:22-32.
30. Kaushik JK, Bhat R. Why is trehalose an exceptional protein stabilizer? An analysis of the thermal stability of proteins in the presence of the compatible osmolyte trehalose. J Biol Chem 2003;278:26458-26465.
31. Loven AD, Olsen AK, Friis C, Andersen B, Phase I and II metabolism and carbohydrate metabolism in cultured cryopreserved porcine hepatocytes. Chem Biol Interact 2005;155:21-30.
32. Acker JP, Fowler A, Lauman B, Cheley S, Toner M. Survival of desiccated mammalian cells: beneficial effects of Isotonic media. Cell Preserv Technol 2002;1:129-140.