Investigation of functional and morphological integrity of freshly isolated and cryopreserved human hepatocytes

Cell and Tissue Banking

Volumn 1, Issue 1, 2000, Pages 55-68

  Ostrowska, Alina ^a,d   Bode, Donald C ^b   Pruss, Jennifer ^a   Bilir, Bahri ^c   Smith, Gina D ^d   Zeisloft, Stephen ^a  

^a Intl Inst for the Adv of Medicine   (United States)

^b SANOFI   (France)

^c Porter Adventist Hospital   (United States)

^d IIAM/T3 ^*

Author keywords

Cell viability; Cryopreservation; Hepatocyte function; Human hepatocytes

Indexed keywords

EID: 0242355262     PISSN: 13899333     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Adams RM, Wang M, Crane AM, Brown B, Darlington GJ and Ledley FD (1995) Effective cryopreservation and long-term storage of primary human hepatocytes with recovery of viability, differentiation, and replicative potential. Cell Trasplant 4: 579-586
2. Barr J, Weir AJ, Brendel K and Sipes IG (1991) Liver slices in dynamic organ culture. II. An in vitro cellular technique for the study of integrated drug metabolism using human tissue. Xenobiotica 21: 341-350
3. Berry MN, Edwards AM and Barritt GJ (1991) Isolated hepatocytes: preparation, properties and applications. In: Burdon RH and Knippenberg PH (eds) Laboratory Techniques in Biochemistry and Molecular Biology, Vol 21, pp 1-460. Elsevier, Amsterdam
4. Bilir B, Ostrowska A, Hanks T and Karrer FM ( 1995) Controlled rate freezing of hepatocytes: a step closer to human hepatocyte banking. Hepatology 22(pt 2): 211A
5. Bode DC (1997) Organization and activities of human tissue banks in the U.S. with special reference to the International Institute for the Advancement of Medicine. Tiss Cult Res Commun 16: 157-163
6. Brandhorst D, Brandhorst H, Hering BJ, Federlin K and Bretzel RG (1999) Large variability of the intracellular ATP content of human islets isolated from different donors. J Mol Med 77: 93-95
7. Chesne C, Guyomard C, Fautrel A, Poullain MG, Fremond B, De Jong H and Guillouzo A (1993) Viability and function in primary culture of adult hepatocytes from various animal species and human beings after cryopreservation. Hepatology 18: 406-414
8. Coundouris JA, Grant MH, Engeset J, Petrie JC and Hawksworth GM (1993) Cryopreservation of human adult hepatocytes for use in drug metabolism and toxicity studies. Xenobiotica 23: 1399-1409
9. De Loecker R, Fuller BJ, Gruwez J and De Loecker W (1990) The effects of cryopreservation on membrane integrity, membrane transport, and protein synthesis in rat hepatocytes. Cryobiology 27: 143-152
10. De Loecker P, Fuller BJ, Koptelov VA and De Loecker W (1993) Metabolic activity of freshly prepared and cryopreserved hepatocytes in monolayer culture. Cryobiology 30: 12-18
11. Denizot F and Lang R (1986) Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods 89: 271-277
12. Dorko K, Freeswick PD, Bartoli F, Cicalese L, Bardsley BA, Tzakis A and Nussler AK ( 1994) A new technique for isolating and culturing human hepatocytes from whole or split livers not used for transplantation. Cell Transplant 3: 387-395
13. Fox IJ, Chowdhury JR, Kaufmann SS, Goertzen TC, Chowdhury NR, Warkentin PI, Dorko K, Sauter BV and Strom SC (1998) Treatment of the Criggler-Najjar syndrome type I with hepatocyte transplantation. N Engl J Med 14: 1422-1426
14. Fujii T, Ha H, Yokoyama H, Hamamoto H, Yoon SH and Hori H (1995) Applications of MTT assay to primary cultured hepatocytes. Biol Pharm Bull 18: 1446-1449
15. Guillouzo A, Morel F, Langouët, Maheo K and Rissel M (1997) Use of hepatocyte cultures for the study of hepatotoxic compounds. J Hepatol 26 (Suppl 2): 73-80
16. Hewitt WR, Corno V, Eguchi S, Kamlot A, Middleton Y, Beeker T, Demetriou AA and Rozga J (1997) Isolation of human hepatocytes from livers rejected for whole organ transplantation. Transplant Proc 29: 1945-1947
17. Hasset C, Laurenzana EM, Sidhu JS and Omiecinski C (1998) Effects of chemical inducers on human microsomal epoxide hydrolase in primary hepatocyte cultures. Biochem Pharmacol 55:1059-1069
18. Howard RB, Christensen AK, Gibbs FA and Pesch LA (1967) The enzymatic preparation of isolated intact parenchymal cells from rat liver. J Cell Biol 35: 675-684
19. de Kanter R, Olinga R Hof I, de Jager M, Verwillegen WA, Slooff MJ, Koster HJ, Meijer DKF and Groothius GMM (1998) A rapid and simple method for cryopreservation of human liver slices. Xenobiotica 28: 225-234
20. Karrer FM, Bilir B, Gill A, Ostrowska A, Stegall M, Wachs M and Kam I (1997) Cryopreserved hepatocytes are poor stimulators of in vitro cytotoxicity. Transplant Proc 29: 1111-1112
21. Li AP, Reith MK, Rasmussen A, Gorski JC, Hall SD, Xu L, Kaminski D and Cheng LK (1997) Primary human hepatocytes as a tool for the evaluation of structure-activity relationship in cytochrome P450 induction potential of xenobiotics: evaluation of rifampin, rifapentine and rifabutin. Chem Biol Interact 107: 17-30
22. Maganto P, Cienfuegos JA, Santamaria L, Rodriguez V, Eroles G, Andres S, Castillo-Olivares JL and Municio AM (1990) Auxiliary liver by transplanted frozen-thawed hepatocyte. J Surg Res 48: 24-32
23. Matsuda H, Yagi T, Matsuoka J, Yamamura H and Tanaka N (1999) Subzero nonfreezing storage of isolated rat hepatocytes in University of Wisconsin solution. Transplantation 67: 186-191
24. Mito M and Kusano M (1993) Hepatocyte transplantation in man. Cell Transplant 2: 65-74
25. Nyberg SL, Shatford RA, Payne WD, Hu WS and Cerra FB (1993) Staining with fluorescein diacetate correlates with hepatocyte function. Biotech Histochem 68: 56-63