Intracellular trehalose improves the survival of cryopreserved mammalian cells

Nature Biotechnology

Volumn 18, Issue 2, 2000, Pages 163-167

  Eroglu, Ali ^a   Russo, Michael J ^a   Bieganski, Robert ^a   Fowler, Alex ^a,b   Cheley, Stephen ^c   Bayley, Hagan ^c   Toner, Mehmet ^a  

^a SHRINERS HOSPITALS FOR CHILDREN   (United States)

^b Department of Electrical and Computer Engineering   (United States)

^c TEXAS A M COLLEGE OF MEDICINE   (United States)

Author keywords

toxin; Cryopreservation; Fibroblasts; Keratinocytes; Trehalose

Indexed keywords

ALPHA TOXIN; HEMOLYSIN; TREHALOSE;

ANIMAL CELL; ARTICLE; CELL MEMBRANE PERMEABILITY; CELL SURVIVAL; CRYOPRESERVATION; FIBROBLAST; FLOW CYTOMETRY; FREEZING; GENE MUTATION; GENETIC ENGINEERING; KERATINOCYTE; MOUSE; NONHUMAN; PRIORITY JOURNAL; STAPHYLOCOCCUS AUREUS; THAWING;

3T3 CELLS; ANIMALS; BACTERIAL TOXINS; BIOLOGICAL TRANSPORT; CELL MEMBRANE; CELL SURVIVAL; CRYOPRESERVATION; CRYOPROTECTIVE AGENTS; GENETIC ENGINEERING; HEMOLYSIN PROTEINS; HUMANS; KERATINOCYTES; MICE; STAPHYLOCOCCUS AUREUS; TREHALOSE;

ANIMALIA; MAMMALIA; STAPHYLOCOCCUS AUREUS;

EID: 0033951109     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/72608     Document Type: Article

References (23)

1. Langer, R. & Vacanti, J.P. Tissue engineering: the development of functional substitutes for damaged tissue. Science 260, 920-926 (1993).
2. Mulligan, R.C. The basic science of gene therapy. Science 260, 926-932 (1993).
3. Mazur, P. Freezing of living cells: mechanisms and implications. Am. J. Physiol. 247, C125-C142 (1984).
4. Carpenter, J.F., Crowe, L.M. & Crowe, J.H. Stabilization of phosphofructokinase with sugars during freeze-drying. Biochim. Biophys. Acta 932, 109-115 (1987).
5. Crowe, L.M., Crowe, J.H., Rudolph, A., Wormersley, C. & Appel, L. Preservation of dried liposomes by trehalose. Arch. Biochem. Biophys. 242, 240-247 (1985).
6. Crowe, L.M., Mouradian, R., Crowe, J.H., Jackson, S.A. & Wormersly, C. Effect of carbohydrates on membrane stability at low water activities. Biochim. Biophys. Acta 769, 141-150 (1984).
7. Green, J.L. & Angell, C.A. Phase-relations and vitrification in saccharide-water solutions and the trehalose anomaly. J. Phys. Chem. 93, 2880-2882 (1989).
8. Bieganski, R.M., Fowler, A., Morgan & J.R. Toner, M. Stabilization of active recombinant retroviruses in an amorphos dry state with trehalose. Biotechnol. Prog. 14, 615-620 (1998).
9. Israeli, E., Shaffer, B.T. & Lighthart, B. Protection of freeze-dried Escherichia coli by trehalose upon exposure to environmental conditions. Cryobiology 30 519-523 (1993).
10. Rossi, S., Buera, M.P., Moreno, S. & Chirife, J. Stabilization of the restriction enzyme EcoRI dried with trehalose and other selected glass-forming solutions. Biotechnol. Prog. 13, 609-616 (1997).
11. Uritani, M., Takai, M. & Yoshinaga, K. Protective effect of disaccharides on restriction endonucleases during drying under vacuum. J. Biochem. 117, 774-779 (1995).
12. Miller, D.P., Pablo, J.J. & Corti, H. Thermophysical properties of trehalose and its concentrated aqueous solutions. Pharm. Res. 14, 578-590 (1997).
13. Russo, M.J., Bayley, H. & Toner, M. Reversible permeabilization of plasma membranes with an engineered switchable pore. Nat. Biotechnol. 15, 278-282 (1997).
14. Walker, B., Kasianowicz, J., Krishnasastry, M. & Bayley, H. A pore-forming protein with a metal-actuated switch. Protein Eng. 7, 655-662 (1994).
15. Song, L. et al. Structure of staphylococcal α-hemolysin, a heptameric transmembrane pore. Science 274, 1859-1866 (1996).
16. Bayley, H. Building doors into cells. Sci. Am. 277, 62-67 (1997).
17. Beattie, G.M. et al. Trehalose: a cryoprotectant that enhances recovery and preserves function of human pancreatic islets after long-term storage. Diabetes 46, 519-523 (1997).
18. Mazur, P., Farrant, J., Leibo, S.P. & Chu, E.H.Y. Survival of hamster tissue culture cells after freezing and thawing. Cryobiology 6, 1-9 (1969).
19. Mazur, P., Leibo, S.P. & Chu, E.H.Y. A two-factor hypothesis of freezing injury. Exp. Cell Res. 71, 345-355 (1972).
20. Walev, I. et al. Staphylococcal alpha-toxin kills human keratinocytes by permeabilizing the plasma membrane for monovalent ions. Infect. Immun. 61, 4972-4979 (1993).
21. Eming, S.A. & Morgan, J.R. in Methods in molecular medicine, gene therapy protocols. (ed. Robbins, P.) 265-279 (Humana, Totowa, NJ; 1997).
22. Hildebrand, A., Pohl, M. & Bhakdi, S. Staphylococcus aureus α-toxin: dual mechanism of binding to target cells. J. Biol. Chem. 266, 17195-17200 (1991).
23. Walker, B., Braha, O., Cheley, S. & Bayley H. An intermediate in the assembly of a pore-forming protein trapped with a genetically engineered switch. Chem. Biol. 2, 99-105 (1995).