Phase I and II metabolism and carbohydrate metabolism in cultured cryopreserved porcine hepatocytes

Chemico-Biological Interactions

Volumn 155, Issue 1-2, 2005, Pages 21-30

  Loven, Anne Dorthe Bonde ^a,b   Olsen, Anette Kristensen ^b   Friis, Christian ^a   Andersen, Birgitte ^b  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b NOVO NORDISK A S   (Denmark)

Author keywords

Cryopreservation; CYP2A; CYP3A; Glucose metabolism; Porcine hepatocytes; UGT

Indexed keywords

4 NITROPHENOL; COUMARIN 7 HYDROXYLASE; CYTOCHROME P450 3A4; GLUCAGON; GLYCOGEN; INSULIN;

ANIMAL CELL; ARTICLE; CARBOHYDRATE METABOLISM; CELL CULTURE; CELL METABOLISM; CELL REGENERATION; CONTROLLED STUDY; CRYOPRESERVATION; FEMALE; FREEZING; GLUCONEOGENESIS; GLUCURONIDATION; GLYCOGEN SYNTHESIS; GLYCOGENOLYSIS; HYDROXYLATION; LIVER CELL; LIVER CELL DAMAGE; NONHUMAN; STIMULATION; SWINE; THAWING;

ANIMALS; ARYL HYDROCARBON HYDROXYLASES; CELL SURVIVAL; CELLS, CULTURED; CRYOPRESERVATION; CYTOCHROME P-450 CYP3A; FEMALE; GLUCOSE; GLUCURONOSYLTRANSFERASE; HEPATOCYTES; OXIDOREDUCTASES, N-DEMETHYLATING; STEROID HYDROXYLASES; SWINE;

SUIDAE;

EID: 20644437301     PISSN: 00092797     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cbi.2005.03.040     Document Type: Article

References (29)

1. M.J. Gomez-Lechon, P. Lopez, and J.V. Castell Biochemical functionality and recovery of hepatocytes after deep freezing storage In vitro 20 1984 826 832
2. N.J. Swales, C. Luong, and J. Caldwell Cryopreservation of rat and mouse hepatocytes. I. Comparative viability studies Drug metabol. disposit. 24 1996 1218 1223
3. N.J. Swales, and D. Utesch Metabolic activity of fresh and cryopreserved dog hepatocyte suspensions Xenobiotica 28 1998 937 948
4. A. Guillouzo, L. Rialland, A. Fautrel, and C. Guyomard Survival and function of isolated hepatocytes after cryopreservation Chem.-Biol. Interact. 121 1999 7 16
5. J.G. Hengstler, M. Ringel, K. Biefang, S. Hammel, U. Milbert, M. Gerl, M. Klebach, B. Diener, K.L. Platt, T. Bottger, P. Steinberg, and F. Oesch Cultures with cryopreserved hepatocytes: applicability for studies of enzyme induction Chem.-Biol. Interact. 125 2000 51 73
6. J.G. Hengstler, D. Utesch, P. Steinberg, K. Platt, B. Diener, M. Ringel, N. Swales, T. Fischer, K. Biefang, M. Gerl, T. Bottger, and F. Oesch Cryopreserved primary hepatocytes as a constantly available in vitro model for the evaluation of human and animal drug metabolism and enzyme induction Drug Metabol. Rev. 32 2000 81 118
7. A.P. Li, P.D. Gorycki, J.G. Hengstler, G.L. Kedderis, H.G. Koebe, R. Rahmani, G. de Sousas, J.M. Silva, and P. Skett Present status of the application of cryopreserved hepatocytes in the evaluation of xenobiotics: consensus of an international expert panel Chem. Biol. Interact. 121 1999 117 123
8. H.G. Koebe, J.C. Dunn, M. Toner, L.M. Sterling, A. Hubel, E.G. Cravalho, M.L. Yarmush, and R.G. Tompkins A new approach to the cryopreservation of hepatocytes in a sandwich culture configuration Cryobiology 27 1990 576 584
9. S. Mahler Hypothermic storage and cryopreservation of hepatocytes: The protective effect of alginate gel against cell damages Cell transplant. 12 2003 579 592
10. C. Guyomard, L. Rialland, B. Fremond, C. Chesne, and A. Guillouzo Influence of alginate gel entrapment and cryopreservation on survival and xenobiotic metabolism capacity of rat hepatocytes Toxicol. Appl. Pharmacol. 141 1996 349 356
11. A. Fautrel, B. Joly, C. Guyomard, and A. Guillouzo Long-term maintenance of drug-metabolizing enzyme activities in rat hepatocytes after cryopreservation Toxicol. Appl. Pharmacol. 147 1997 110 114
12. A. Madan, R. Dehaan, D. Mudra, K. Carroll, E. LeCluyse, and A. Parkinson Effect of cryopreservation on cytochrome P-450 enzyme induction in cultured rat hepatocytes Drug Metabol. Disposit. 27 1999 327 335
13. N.J. Swales, T. Johnson, and J. Caldwell Cryopreservation of rat and mouse hepatocytes. II. Assessment of metabolic capacity using testosterone metabolism Drug Metabol. Disposit. 24 1996 1224 1230
14. A.P. Li, C. Lu, J.A. Brent, C. Pham, A. Fackett, C.E. Ruegg, and P.M. Silber Cryopreserved human hepatocytes: characterization of drug-metabolizing enzyme activities and applications in higher throughput screening assays for hepatotoxicity, metabolic stability, and drug-drug interaction potential Chem. Biol. Interact. 121 1999 17 35
15. N.J. Hewitt, T. Fischer, U. Zuehlke, F. Oesch, and D. Utesch Metabolic activity of fresh and cryopreserved cynomolgus monkey (Macaca fascicularis) hepatocytes Xenobiotica 30 2000 665 681
16. K.J. Dabos, J.A. Parkinson, C. Hewage, L.J. Nelson, I.H. Sadler, P.C. Hayes, and J.N. Plevris H-1 NMR spectroscopy as a tool to evaluate key metabolic functions of primary porcine hepatocytes after cryopreservation NMR Biomed. 15 2002 241 250
17. A. Le Cam, A. Guillouzo, and P. Freychet Ultrastructural and biochemical studies of isolated adult rat hepatocytes prepared under hypoxic conditions. Cryopreservation of hepatocytes Exp. Cell Res. 98 1976 382 395
18. P. Skett, P. Roberts, and S. Khan Maintenance of steroid metabolism and hormone responsiveness in cryopreserved dog, monkey and human hepatocytes Chem. Biol. Interact. 121 1999 65 76
19. V.Y. Toshchakov, G.I. Morozova, and N.A. Onishchenko Energy metabolism of cryopreserved rat hepatocytes Cryo Lett. 12 1991 259 272
20. A.K. Olsen, K.T. Hansen, and C. Friis Pig hepatocytes as an in vitro model to study the regulation of human CYP3A4: prediction of drug-drug interactions with 17 alpha-ethynylestradiol Chem. Biol. Interact. 107 1997 93 108
21. Z.Z. O'Brien, M. Hann, Y.H. Lee, K. Holme, Effect of media temperature and percoll centrifugation on significant improvement of cell viability of human cryopreserved primary hepatocytes, Hepatocyte Users Group meeting, Stansted, England, poster no. 10, 2001.
22. M.T. Skaanild Cytochrome P450 sex differences in minipigs and conventional pigs Pharmacol. Toxicol. 85 1999 174 180
23. P.G. Gregory, C.K. Connolly, M. Toner, and S.J. Sullivan In vitro characterization of porcine hepatocyte function Cell Transplant. 9 2000 1 10
24. M.T. Skaanild, and C. Friis Expression changes of CYP2A and CYP3A in microsomes from pig liver and cultured hepatocytes Pharmacol. Toxicol. 87 2000 174 178
25. J.M. Pascussi, L. Drocourt, J.M. Fabre, P. Maurel, and M.J. Vilarem Dexamethasone induces pregnane X receptor and retinoid X receptor-alpha expression in human hepatocytes: Synergistic increase of CYP3A4 induction by pregnane X receptor activators Mol. Pharmacol. 58 2000 361 372
26. M. Monshouwer, G.A.E. Van't Klooster, S.M. Nijmeijer, R.F. Witkamp, and A.S.P.A. Van Miert Characterization of cytochrome P450 isoenzymes in primary cultures of pig hepatocytes Toxicol. In Vitro 12 1998 715 723
27. N. Hanioka, H. Jinno, T. Tanaka-Kagawa, T. Nishimura, and M. Ando Determination of UDP-glucuronosyltransferase UGT1A6 activity in human and rat liver microsomes by HPLC with UV detection J. Pharmaceut. Biomed. Anal. 25 2001 65 75
28. P.I. Mackenzie Regulation of UDP glucuronosyltransferase genes Curr. Drug Metabol. 4 2003 249 257
29. J.M. Pang Actions of selected hepatotoxicants on freshly isolated and cryopreserved rat hepatocytes Cryobiology 33 1996 226 235