Overexpression, purification and characterization of a hexahistidine-tagged recombinant extended nucleotide-binding domain 1 (NBD1) of the Cryptosporidium parvum CpABC3 for rational drug design

Molecular and Biochemical Parasitology

Volumn 152, Issue 1, 2007, Pages 101-107

  Lawton, Philippe ^a   Pélandakis, Michel ^a   Pétavy, Anne Françoise ^a   Walchshofer, Nadia ^a  

^a UNIVERSITÉ LYON 1   (France)

Author keywords

CpABC3; Cryptosporidium parvum; NBD1; Nucleotide binding domain; Parasite

Indexed keywords

ABC TRANSPORTER; ABC TRANSPORTER 3; ADENOSINE DIPHOSPHATE; ADENOSINE PHOSPHATE; ADENOSINE TRIPHOSPHATE; ADENOSINE TRIPHOSPHATE DERIVATIVE; FLAVONE; HEXAHISTIDINE; PROGESTERONE; PROTOZOAL PROTEIN; QUERCETIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING KINETICS; BINDING SITE; CATALYSIS; COMPETITIVE INHIBITION; CONCENTRATION RESPONSE; CONTROLLED STUDY; CRYPTOSPORIDIUM PARVUM; DRUG DESIGN; ESCHERICHIA COLI; FLUORESCENCE ANALYSIS; GENE OVEREXPRESSION; HYDROLYSIS; HYDROPHOBICITY; MOLECULAR WEIGHT; NONHUMAN; NONPHOTOCHEMICAL QUENCHING; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN PURIFICATION;

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; AMINO ACID SEQUENCE; ANIMALS; CLONING, MOLECULAR; CRYPTOSPORIDIUM PARVUM; DRUG DESIGN; ENZYME INHIBITORS; ESCHERICHIA COLI; GENE EXPRESSION; MOLECULAR SEQUENCE DATA; P-GLYCOPROTEINS; PROGESTERONE; PROTEIN STRUCTURE, TERTIARY; PROTOZOAN PROTEINS; QUERCETIN; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

CRYPTOSPORIDIUM PARVUM; PROTOZOA;

EID: 33846235457     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2006.12.004     Document Type: Article

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