Smurf1 regulates tumor cell plasticity and motility through degradation of RhoA leading to localized inhibition of contractility

Journal of Cell Biology

Volumn 176, Issue 1, 2007, Pages 35-42

  Sahai, Erik ^b   Garcia Medina, Raquel ^a   Pouysségur, Jacques ^a   Vial, Emmanuel ^a  

^a CNRS   (France)

^b IMPERIAL CANCER RESEARCH FUND   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

MYOSIN LIGHT CHAIN; MYOSIN LIGHT CHAIN 2; RHO KINASE; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; SMURF1 ENZYME; UBIQUITIN PROTEIN LIGASE E3; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ARTICLE; CELL MOTILITY; CONTROLLED STUDY; DOWN REGULATION; ENZYME ACTIVITY; HUMAN; HUMAN CELL; IN VIVO STUDY; LAMELLIPODIUM; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PHOSPHORYLATION; TISSUE CULTURE; TUMOR CELL;

ANIMALS; CARDIAC MYOSINS; CELL MOVEMENT; ENZYME ACTIVATION; GENE SILENCING; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MESODERM; MICE; MYOSIN LIGHT CHAINS; NEOPLASM INVASIVENESS; NEOPLASMS; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN-SERINE-THREONINE KINASES; PSEUDOPODIA; RHOA GTP-BINDING PROTEIN; SIGNAL TRANSDUCTION; UBIQUITIN-PROTEIN LIGASES;

EID: 33846014269     PISSN: 00219525     EISSN: 00219525     Source Type: Journal    
DOI: 10.1083/jcb.200605135     Document Type: Article

References (27)

1. Berra, E., E. Benizri, A. Ginouves, V. Volmat, D. Roux, and J. Pouyssegur. 2003. HIF prolyl-hydroxylase 2 is the key oxygen sensor setting low steady-state levels of HIF-1alpha in normoxia. EMBO J. 22:4082-4090.
2. Clark, E.A., T.R. Golub, E.S. Lander, and R.O. Hynes. 2000. Genomic analysis of metastasis reveals an essential role for RhoC. Nature. 406:532-535.
3. Cox, E.A., S.K. Sastry, and A. Huttenlocher. 2001. Integrin-mediated adhesion regulates cell polarity and membrane protrusion through the Rho family of GTPases. Mol. Biol. Cell. 12:265-277.
4. Croft, D.R., E. Sahai, G. Mavria, S. Li, J. Tsai, W.M. Lee, C.J. Marshall, and M.F. Olson. 2004. Conditional ROCK activation in vivo induces tumor cell dissemination and angiogenesis. Cancer Res. 64:8994-9001.
5. Friedl, P., and K. Wolf. 2003. Tumour-cell invasion and migration: diversity and escape mechanisms. Nat. Rev. Cancer. 3:362-374.
6. Goulimari, P., T.M. Kitzing, H. Knieling, D.T. Brandt, S. Offermanns, and R. Grosse. 2005. Galpha12/13 is essential for directed cell migration and localized Rho-Dia1 function. J. Biol. Chem. 280:42242-42251.
7. Hakem, A., O. Sanchez-Sweatman, A. You-Ten, G. Duncan, A. Wakeham, R. Khokha, and T.W. Mak. 2005. RhoC is dispensable for embryogenesis and tumor initiation but essential for metastasis. Genes Dev. 19:1974-1979.
8. Hall, A. 1998. Rho GTPases and the actin cytoskeleton. Science. 279:509-514.
9. Izzi, L., and L. Attisano. 2004. Regulation of the TGFbeta signalling pathway by ubiquitin-mediated degradation. Oncogene. 23:2071-2078.
10. Malliri, A., M. Symons, R.F. Hennigan, A.F. Hurlstone, R.F. Lamb, T. Wheeler, and B.W. Ozanne. 1998. The transcription factor AP-1 is required for EGF-induced activation of Rho-like GTPases, cytoskeletal rearrangements, motility, and in vitro invasion of A431 cells. J. Cell Biol. 143:1087-1099.
11. Ozdamar, B., R. Bose, M. Barrios-Rodiles, H.R. Wang, Y. Zhang, and J.L. Wrana. 2005. Regulation of the polarity protein Par6 by TGFbeta receptors controls epithelial cell plasticity. Science. 307:1603-1609.
12. Ridley, A.J. 2001. Rho GTPases and cell migration. J. Cell Sci. 114:2713-2722.
13. Sahai, E. 2005. Mechanisms of cancer cell invasion. Curr. Opin. Genet. Dev. 15:87-96.
14. Sahai, E., and C.J. Marshall. 2002. RHO-GTPases and cancer. Nat. Rev. Cancer. 2:133-142.
15. Sahai, E., and C.J. Marshall. 2003. Differing modes of tumour cell invasion have distinct requirements for Rho/ROCK signalling and extracellular proteolysis. Nat. Cell Biol. 5:711-719.
16. Sahai, E., and M.F. Olson. 2006. Purification of TAT-C3 exoenzyme. Methods Enzymol. 406:128-140.
17. Sahai, E., M.F. Olson, and C.J. Marshall. 2001. Cross-talk between Ras and Rho signalling pathways in transformation favours proliferation and increased motility. EMBO J. 20:755-766.
18. Sahai, E., J. Wyckoff, U. Philippar, J.E. Segall, F. Gertler, and J. Condeelis. 2005. Simultaneous imaging of GFP, CFP and collagen in tumors in vivo using multiphoton microscopy. BMC Biotechnol. 5:14.
19. Suzuki, C., G. Murakami, M. Fukuchi, T. Shimanuki, Y. Shikauchi, T. Imamura, and K. Miyazono. 2002. Smurf1 regulates the inhibitory activity of Smad7 by targeting Smad7 to the plasma membrane. J. Biol. Chem. 277:39919-39925.
20. van de Wetering, M., I. Oving, V. Muncan, M.T. Pon Fong, H. Brantjes, D. van Leenen, F.C. Holstege, T.R. Brummelkamp, R. Agami, and H. Clevers. 2003. Specific inhibition of gene expression using a stably integrated, inducible small-interfering-RNA vector. EMBO Rep. 4:609-615.
21. Vial, E., E. Sahai, and C.J. Marshall. 2003. ERK-MAPK signaling coordinately regulates activity of Rac1 and RhoA for tumor cell motility. Cancer Cell. 4:67-79.
22. Wang, H.R., Y. Zhang, B. Ozdamar, A.A. Ogunjimi, E. Alexandrova, G.H. Thomsen, and J.L. Wrana. 2003. Regulation of cell polarity and protrusion formation by targeting RhoA for degradation. Science. 302:1775-1779.
23. Wang, W., J.B. Wyckoff, V.C. Frohlich, Y. Oleynikov, S. Huttelmaier, J. Zavadil, L. Cermak, E.P. Bottinger, R.H. Singer, J.G. White, et al. 2002. Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling. Cancer Res. 62:6278-6288.
24. Wilkinson, S., H.F. Paterson, and C.J. Marshall. 2005. Cdc42-MRCK and Rho-ROCK signalling cooperate in myosin phosphorylation and cell invasion. Nat. Cell Biol. 7:255-261.
25. Worthylake, R.A., and K. Burridge. 2003. RhoA and ROCK promote migration by limiting membrane protrusions. J. Biol. Chem. 278:13578-13584.
26. Wyckoff, J.B., S.E. Pinner, S. Gschmeissner, J.S. Condeelis, and E. Sahai. 2006. ROCK- and myosin-dependent matrix deformation enables protease-independent tumor-cell invasion in vivo. Curr. Biol. 16:1515-1523.
27. Yamashita, M., S.X. Ying, G.M. Zhang, C. Li, S.Y. Cheng, C.X. Deng, and Y.E. Zhang. 2005. Ubiquitin ligase Smurf1 controls osteoblast activity and bone homeostasis by targeting MEKK2 for degradation. Cell. 121:101-113.