Stage-specific conditional mutagenesis in mouse embryonic stem cell-derived neural cells and postmitotic neurons by direct delivery of biologically active cre recombinase

Stem Cells

Volumn 25, Issue 1, 2007, Pages 181-188

  Haupt, Simone ^a   Edenhofer, Frank ^a   Peitz, Michael ^a   Leinhaas, Anke ^a   Brüstle, Oliver ^a  

^a UNIVERSITY OF BONN   (Germany)

Author keywords

Conditional mutagenesis; Neural differentiation; Neural repair; Protein transduction; Site specific recombination; Stem cell therapy

Indexed keywords

CRE RECOMBINASE; GREEN FLUORESCENT PROTEIN;

ANIMAL CELL; ARTICLE; CELL MEMBRANE PERMEABILITY; CELL PROLIFERATION; CONDITIONAL MUTAGENESIS; EMBRYONIC STEM CELL; GENETIC RECOMBINATION; GENETIC TRANSDUCTION; GLIA CELL; IN VITRO STUDY; INDUCED MUTATION; MITOSIS; MOUSE; MUTAGENESIS; NERVE CELL; NERVE CELL DIFFERENTIATION; NEURAL STEM CELL; NONHUMAN; PROTEIN EXPRESSION;

ANIMALS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL DIVISION; EMBRYONIC STEM CELLS; GENE TRANSFER TECHNIQUES; INTEGRASES; MICE; MITOSIS; MUTAGENESIS; NEURONS; RECOMBINANT PROTEINS;

EID: 33846004741     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1634/stemcells.2006-0371     Document Type: Article

References (34)

1. Branda CS, Dymecki SM. Talking about a revolution: The impact of site-specific recombinases on genetic analyses in mice. Dev Cell 2004;6:7-28.
2. Lewandoski M. Conditional control of gene expression in the mouse. Nat Rev Genet 2001;2:743-755.
3. Garcia-Otin AL, Guillou F. Mammalian genome targeting using site-specific recombinases. Front Biosci 2006;11:1108-1136.
4. Chambers I, Colby D, Robertson M et al. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell 2003;113:643-655.
5. Schindehutte J, Fukumitsu H, Collombat P et al. In vivo and in vitro tissue-specific expression of green fluorescent protein using the cre-lox system in mouse embryonic stem cells. STEM CELLS 2005;23:10-15.
6. Tan SM, Droge P. Comparative analysis of sequence-specific DNA recombination systems in human embryonic stem cells. STEM CELLS 2005;23:868-873.
7. Ren X, Katoh M, Hoshiya H et al. A novel human artificial chromosome vector provides effective cell lineage-specific transgene expression in human mesenchymal stem cells. STEM CELLS 2005;23:1608-1616.
8. Gu H, Zou YR, Rajewsky K. Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting. Cell 1993;73:1155-1164.
9. Gagneten S, Le Y, Miller J, Sauer B. Brief expression of a GFP cre fusion gene in embryonic stem cells allows rapid retrieval of site-specific genomic deletions. Nucleic Acids Res 1997;25:3326-3331.
10. Kanegae Y, Lee G, Sato Y et al. Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res 1995;23:3816-3821.
11. Kaspar BK, Vissel B, Bengoechea T et al. Adeno-associated virus effectively mediates conditional gene modification in the brain. Proc Natl Acad Sci U S A 2002;99:2320-2325.
12. Ahmed BY, Chakravarthy S, Eggers R et al. Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors. BMC Neurosci 2004;5:4.
13. Metzger D, Clifford J, Chiba H et al. Conditional site-specific recombination in mammalian cells using a ligand-dependent chimeric Cre recombinase. Proc Natl Acad Sci U S A 1995;92:6991-6995.
14. Kellendonk C, Tronche F, Monaghan AP et al. Regulation of Cre recombinase activity by the synthetic steroid RU 486. Nucleic Acids Res 1996;24:1404-1411.
15. Wunderlich FT, Wildner H, Rajewsky K et al. New variants of inducible Cre recombinase: A novel mutant of Cre-PR fusion protein exhibits enhanced sensitivity and an expanded range of inducibility. Nucleic Acids Res 2001;29:E47.
16. Washbourne P, McAllister AK. Techniques for gene transfer into neurons. Curr Opin Neurobiol 2002;12:566-573.
17. Berry M, Barrett L, Seymour L et al. Gene therapy for central nervous system repair. Curr Opin Mol Ther 2001;3:338-349.
18. Dietz GP, Bahr M. Delivery of bioactive molecules into the cell: The Trojan horse approach. Mol Cell Neurosci 2004;27:85-131.
19. Peitz M, Pfannkuche K, Rajewsky K et al. Ability of the hydrophobic FGF and basic TAT peptides to promote cellular uptake of recombinant Cre recombinase: A tool for efficient genetic engineering of mammalian genomes. Proc Natl Acad Sci U S A 2002;99:4489-4494.
20. Nolden L, Edenhofer F, Haupt S et al. Site specific recombination in human embryonic stem cells induced by cell permeable Cre recombinase. Nat Methods 2006;6:461-467.
21. Novak A, Guo C, Yang W et al. Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon Cre-mediated excision. Genesis 2000;28:147-155.
22. Brüstle O, Jones KN, Learish RD et al. Embryonic stem cell-derived glial precursors: A source of myelinating transplants. Science 1999;285:754-756.
23. Okabe S, Forsberg-Nilsson K, Spiro AC et al. Development of neuronal precursor cells and functional postmitotic neurons from embryonic stem cells in vitro. Mech Dev 1996;59:89-102.
24. Brüstle O, Spiro CA, Karram K et al. In vitro-generated neural precursors participate in mammalian brain development. Proc Natl Acad Sci U S A 1997;94:14809-14814.
25. Glover DJ, Lipps HJ, Jans DA. Towards safe, non-viral therapeutic gene expression in humans. Nat Rev Genet 2005;6:299-310.
26. Li M, Pevny L, Lovell-Badge R et al. Generation of purified neural precursors from embryonic stem cells by lineage selection. Curr Biol 1998;8:971-974.
27. Wernig M, Tucker KL, Gornik V et al. Tau EGFP embryonic stem cells: An efficient tool for neuronal lineage selection and transplantation. J Neurosci Res 2002;69:918-924.
28. Schmandt T, Meents E, Gossrau G et al. High-purity lineage selection of embryonic stem cell-derived neurons. Stem Cells Dev 2005;14:55-64.
29. Glaser T, Perez-Bouza A, Klein K et al. Generation of purified oligodendrocyte progenitors from embryonic stem cells. FASEB J 2005;19:112-114.
30. Chung S, Sonntag KC, Andersson T et al. Genetic engineering of mouse embryonic stem cells by Nurr1 enhances differentiation and maturation into dopaminergic neurons. Eur J Neurosci 2002;16:1829-1838.
31. Kim JH, Auerbach JM, Rodriguez-Gomez JA et al. Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson's disease. Nature 2002;418:50-56.
32. Andersson E, Tryggvason U, Deng Q et al. Identification of intrinsic determinants of midbrain dopamine neurons. Cell 2006;124:393-405.
33. Watase K, Zoghbi HY. Modelling brain diseases in mice: The challenges of design and analysis. Nat Rev Genet 2003;4:296-307.
34. Morozov A, Kellendonk C, Simpson E et al. Using conditional mutagenesis to study the brain. Biol Psychiatry 2003;54:1125-1133.