Isolation of a novel Thermus thermophilus metal efflux protein that improves Escherichia coli growth under stress conditions

Extremophiles

Volumn 6, Issue 4, 2002, Pages 301-308

  Spada, Stefania ^a   Pembroke, J Tony ^a   Wall, J Gerard ^a  

^a UNIVERSITY OF LIMERICK   (Ireland)

Author keywords

CzrB; Heavy metal efflux; Stress reduction; Thermus thermophilus

Indexed keywords

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA; THERMUS; THERMUS THERMOPHILUS;

BACTERIAL PROTEIN; CZRB PROTEIN, BACTERIA; MEMBRANE PROTEIN; PRIMER DNA;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; SEQUENCE HOMOLOGY; THERMUS THERMOPHILUS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; SEQUENCE HOMOLOGY, AMINO ACID; THERMUS THERMOPHILUS;

EID: 0036691789     PISSN: 14310651     EISSN: 14334909     Source Type: Journal    
DOI: 10.1007/s00792-001-0257-0     Document Type: Article

References (35)

1. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389-3402
2. Anton A, Grosse C, Reissmann J, Pribyl T, Nies DH (1999) CzcD is a heavy metal ion transporter involved in regulation of heavy metal resistance in Ralstonia sp. strain CH34. J Bacteriol 181:6876-6881
3. Beard SJ, Hashim R, Membrillo-Hernández J, Hughes MN, Poole RK (1997) Zinc(II) tolerance in Escherichia coli K-12: evidence that the zntA gene (o732) encodes a cation transport ATPase. Mol Microbiol 25:883-891
4. Bothmann H, Plückthun A (1998) Selection for a periplasmic factor improving phage display and functional periplasmic expression. Nat Biotechnol 16:376-380
5. Bullock WO, Fernandez JM, Short JM (1987) XL1-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with β-galactosidase selection. BioTechniques 5:376-379
6. Conklin DS, Culbertson MR, Kung C (1994) Interactions between gene products involved in divalent cation transport in Saccharomyces cerevisiae. Mol Gen Genet 244:303-311
7. Grass G, Fan B, Rosen BP, Franke S, Nies DH, Rensing C (2001) ZitB (YbgR), a member of the cation diffusion facilitator family, is an essential zinc transporter in Escherichia coli. J Bacteriol 183:4664-4667
8. Hassan M-e-T, van der Lelie D, Springael D, Römling U, Ahmed N, Mergeay M (1999) Identification of a gene cluster, czr, involved in cadmium and zinc resistance in Pseudomonas aeruginosa. Gene (Amst) 238:417-425
9. Hughes MN, Poole RK (1991) Metal speciation and microbial growth - the hard (and soft) facts. J Gen Microbiol 137:725-734
10. Kamizono A, Nishizawa M, Teranishi Y, Murata K, Kimura A (1989) Identification of a gene conferring resistance to zinc and cadmium ions in the yeast Saccharomyces cerevisiae. Mol Gen Genet 219:161-167
11. Knappik A, Plückthun A (1995) Engineered turns of a recombinant antibody improve its in vivo folding. Protein Eng 8:81-89
12. Krogh A, Larsson B, von Heijne G, Sonnhammer ELL (2001) Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol 305:567-580
13. Kumar S, Tsai C-J, Nussinov R (2000) Factors enhancing protein thermostability. Protein Eng 13:179-191
14. Kuroda M, Hayashi H, Ohta T (1999) Chromosome-determined zinc-responsible operon czr in Staphylococcus aureus strain 912. Microbiol Immunol 43:115-125
15. McCafferty J, Griffiths AD, Winter G, Chiswell DJ (1990) Phage antibodies - filamentous phage displaying antibody variable domains. Nature (Lond) 348:552-554
16. Nakai K, Kanehisa M (1992) A knowledge base for predicting protein localization sites in eukaryotic cells. Genomics 14:897-911
17. Nies DH (1992) CzcR and CzcD, gene products affecting regulation of resistance to cobalt, cadmium and zinc (czc system) in Alcaligenes eutrophus. J Bacteriol 174:8102-8110
18. Nies DH (1999) Microbial heavy-metal resistance. Appl Microbiol Biotechnol 51:730-750
19. Nies DH, Silver S (1995) Ion efflux systems involved in bacterial metal resistances. J Ind Microbiol 14:186-199
20. Nies DH, Nies A, Chu L, Silver S (1989) Expression and nucleotide sequences of a plasmid-determined divalent cation efflux system from Alcaligenes eutrophus. Proc Natl Acad Sci USA 86:7351-7355
21. Palmiter RD, Findley SD (1995) Cloning and functional characterization of a mammalian zinc transporter that confers resistance to zinc. EMBO J 14:639-649
22. Paulsen IT, Saier MH Jr (1997) A novel family of ubiquitous heavy metal ion transport proteins. J Membr Biol 156:99-103
23. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Press, New York
24. Silver S, Phung LT (1996) Bacterial heavy metal resistances: new surprises. Annu Rev Microbiol 50:753-789
25. Silver S, Misra TK, Laddaga RA (1989) Bacterial resistance to toxic heavy metals. In: Beveridge TJ, Doyle RJ (eds) Metal ions and bacteria. Wiley, New York, pp 121-139
26. Singh VK, Xiong A, Usgaard TR, Chakrabarti S, Deora R, Misra TK, Jayaswal RK (1999) ZntR is an autoregulatory protein and negatively regulates the chromosomal zinc resistance operon znt of Staphylococcus aureus. Mol Microbiol 33:200-207
27. Spada S, Gibert Y, Pembroke JT, Wall JG (2001) Isolation and characterisation of the ylmE homologue of Thermus thermophilus. DNA Sequence 11:507-514
28. Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FSL, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GKS, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock REW, Lory S, Olson MV (2000) Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen. Nature (Lond) 406:959-964
29. Sturr MG, Ablooglu AJ, Krulwich TA (1997) A Bacillus subtilis locus encoding several gene products affecting transport of cations. Gene (Amst) 188:91-94
30. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTALW: improving the sensitivity of progressive multiple alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acid Res 22:4673-4680
31. van der Lelie D, Schwuchow T, Schwidetzky U, Wuertz S, Baeyens W, Mergeay M, Nies DH (1997) Two-component regulatory system involved in transcriptional control of heavy-metal homeostasis in Alcaligenes eutrophus. Mol Microbiol 23:493-503
32. Wall JG, Plückthun A (1999) The hierarchy of mutations influencing the folding of antibody domains in Escherichia coli. Protein Eng 12:605-611
33. Wilquet V, van de Casteele M (1999) The role of the codon first letter in the relationship between genomic GC content and protein amino acid composition. Res Microbiol 150:21-32
34. Xiong A, Jayaswal RK (1998) Molecular characterization of a chromosomal determinant conferring resistance to zinc and cobalt ions in Staphylococcus aureus. J Bacteriol 180:4024-4029
35. Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene (Amst) 33:103-119