The splicing regulators Tra and Tra2 are unusually potent activators of pre-mRNA splicing

Nucleic Acids Research

Volumn 34, Issue 22, 2006, Pages 6612-6620

  Sciabica, Kathryn S ^a   Hertel, Klemens J ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DROSOPHILA PROTEIN; HYBRID PROTEIN; MESSENGER RNA; PROTEIN 9G8; PROTEIN TRA; PROTEIN TRA2; RECOMBINANT PROTEIN; REGULATOR PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CONTROLLED STUDY; DROSOPHILA; FEMALE; GENE ACTIVATION; GENE DELETION; GENE EXPRESSION REGULATION; GENE MUTATION; GENE SEQUENCE; GENETIC CODE; GENETIC SELECTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN DEPHOSPHORYLATION; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN SYNTHESIS REGULATION; RNA SPLICING; SEX DIFFERENTIATION; SIGNAL TRANSDUCTION; SPLICEOSOME;

ALTERNATIVE SPLICING; ANIMALS; DNA-BINDING PROTEINS; DROSOPHILA PROTEINS; FEMALE; HUMANS; NUCLEAR PROTEINS; PROTEIN STRUCTURE, TERTIARY; REPETITIVE SEQUENCES, NUCLEIC ACID; RIBONUCLEOPROTEINS; RNA PRECURSORS; RNA, MESSENGER; SPLICEOSOMES;

EID: 33845899654     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkl984     Document Type: Article

References (44)

1. Smith,C.W. and Valcarcel,J. (2000) Alternative pre-mRNA splicing: The logic of combinatorial control. Trends Biochem. Sci., 25, 381-388.
2. Black,D.L. (2003) Mechanisms of alternative pre-messenger RNA splicing. Annu. Rev. Biochem., 72, 291-336.
3. Pagani,F. and Baralle,F.E. (2004) Genomic variants in exons and introns: identifying the splicing spoilers. Nature Rev. Genet., 5, 389-396.
4. Blencowe,B.J. (2000) Exonic splicing enhancers: Mechanism of action, diversity and role in human genetic diseases. Trends Biochem. Sci., 25, 106-110.
5. Graveley,B.R. (2000) Sorting out the complexity of SR protein functions. RNA, 6, 1197-1211.
6. Graveley,B.R., Hertel,K.J. and Maniatis,T. (2001) The role of U2AF35 and U2AF65 in enhancer-dependent splicing. RNA, 7, 806-818.
7. Cartegni,L., Chew,S.L. and Krainer,A.R. (2002) Listening to silence and understanding nonsense: Exonic mutations that affect splicing. Nature Rev. Genet., 3, 285-298.
8. Fu,X.D. and Maniatis,T. (1990) Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus. Nature, 343, 437-441.
9. Krainer,A.R., Conway,G.C. and Kozak,D. (1990) Purification and characterization of pre-mRNA splicing factor SF2 from HeLa cells. Genes Dev., 4, 1158-1171.
10. Ge,H. and Manley,J.L. (1990) A protein factor, ASF, controls cell-specific alternative splicing of SV40 early pre-mRNA in vitro. Cell, 62, 25-34.
11. Zahler,A.M., Lane,W.S., Stolk,J.A. and Roth,M.B. (1992) SR proteins: A conserved family of pre-mRNA splicing factors. Genes Dev., 6, 837-847.
12. Fu,X.-D. (1993) Specific commitment of different pre-mRNAs to splicing by single SR proteins. Nature, 365, 82-85.
13. Roscigno,R.F. and Garcia-Blanco,M.A. (1995) SR proteins escort the U4/ U6.U5 tri-snRNP to the spliceosome. RNA, 1, 692-706.
14. Chew,S.L., Liu,H.X., Mayeda,A. and Krainer,A.R. (1999) Evidence for the function of an exonic splicing enhancer after the first catalytic step of pre-mRNA splicing. Proc. Natl Acad. Sci. USA, 96, 10655-10660.
15. Mermoud,J.E., Cohen,P. and Lamond,A.I. (1992) Ser/Thr-specific protein phosphatases are required for both catalytic steps of pre-mRNA splicing. Nucleic Acids Res., 20, 5263-5269.
16. Mermoud,J.E., Cohen,P.T. and Lamond,A.I. (1994) Regulation of mammalian spliceosome assembly by a protein phosphorylation mechanism. EMBO J., 13, 5679-5688.
17. Schaal,T.D. and Maniatis,T. (1999) Multiple distinct splicing enhancers in the protein-coding sequences of a constitutively spliced pre-mRNA. Mol. Cell. Biol., 19, 261-273.
18. Fairbrother,W.G., Yeh,R.F., Sharp,P.A. and Burge,C.B. (2002) Predictive identification of exonic splicing enhancers in human genes. Science, 297, 1007-1013.
19. Shen,H., Kan,J.L. and Green,M.R. (2004) Arginine-serine-rich domains bound at splicing enhancers contact the branchpoint to promote prespliceosome assembly. Mol. Cell, 13, 367-376.
20. Shen,H. and Green,M.R. (2004) A pathway of sequential arginine-serine-rich domain-splicing signal interactions during mammalian spliceosome assembly. Mol. Cell, 16, 363-373.
21. Hertel,K.J., Lynch,K.W. and Maniatis,T. (1997) Common themes in the function of transcription and splicing enhancers. Curr. Opin. Cell Biol., 9, 350-357.
22. Graveley,B.R., Hertel,K.J. and Maniatis,T. (1999) SR proteins are 'locators' of the RNA splicing machinery [letter; comment]. Curr. Biol., 9, R6-R7.
23. Lam,B.J. and Hertel,K.J. (2002) A general role for splicing enhancers in exon definition. RNA, 8, 1233-1241.
24. Lam,B.J., Bakshi,A., Ekinci,F.Y., Webb,J., Graveley,B.R. and Hertel,K.J. (2003) Enhancer-dependent 50-splice site control of fruitless pre-mRNA splicing. J. Biol. Chem., 278, 22740-22747.
25. Graveley,B.R. and Maniatis,T. (1998) Arginine/serine-rich domains of SR proteins can function as activators of pre-mRNA splicing. Mol. Cell, 1, 765-771.
26. Graveley,B.R., Hertel,K.J. and Maniatis,T. (1998) A systematic analysis of the factors that determine the strength of pre-mRNA splicing enhancers. EMBO J., 17, 6747-6756.
27. Baker,B.S. (1989) Sex in flies: The splice of life. Nature, 340, 521-524.
28. Ryner,L.C. and Baker,B.S. (1991) Regulation of doublesex pre-mRNA processing occurs by 3′-splice site activation. Genes Dev., 5, 2071-2085.
29. Ryner,L.C., Goodwin,S.F., Castrillon,D.H., Anand,A., Villella,A., Baker,B.S., Hall,J.C., Taylor,B.J. and Wasserman,S.A. (1996) Control of male sexual behavior and sexual orientation in Drosophila by the fruitless gene. Cell, 87, 1079-1089.
30. McKeown,M., Belote,J.M. and Boggs,R.T. (1988) Ectopic expression of the female transformer gene product leads to female differentiation of chromosomally male Drosophila. Cell, 53, 887-895.
31. Nagoshi,R.N., McKeown,M., Burtis,K.C., Belote,J.M. and Baker,B.S. (1988) The control of alternative splicing at genes regulating sexual differentiation in D. melanogaster. Cell, 53, 229-236.
32. Nagoshi,R.N. and Baker,B.S. (1990) Regulation of sex-specific RNA splicing at the Drosophila doublesex gene: Cis-acting mutations in exon sequences alter sex-specific RNA splicing patterns. Genes Dev., 4, 89-97.
33. Heinrichs,V., Ryner,L.C. and Baker,B.S. (1998) Regulation of sex-specific selection of fruitless 5′ splice sites by transformer and transformer-2. Mol. Cell. Biol., 18, 450-458.
34. Lynch,K.W. and Maniatis,T. (1996) Assembly of specific SR protein complexes on distinct regulatory elements of the Drosophila doublesex splicing enhancer. Genes Dev., 10, 2089-2101.
35. Hertel,K.J. and Maniatis,T. (1998) The function of multisite splicing enhancers. Mol. Cell, 1, 449-455.
36. Hicks,M.J., Lam,B.J. and Hertel,K.J. (2005) Analyzing mechanisms of alternative pre-mRNA splicing using in vitro splicing assays. Methods, 37, 306-313.
37. Tian,M. and Maniatis,T. (1992) Positive control of pre-mRNA splicing in vitro. Science, 256, 237-240.
38. Haynes,C. and Iakoucheva,L.M. (2006) Serine/arginine-rich splicing factors belong to a class of intrinsically disordered proteins. Nucleic Acids Res., 34, 305-312.
39. Zhu,J. and Krainer,A.R. (2000) Pre-mRNA splicing in the absence of an SR protein RS domain. Genes Dev., 14, 3166-3178.
40. Eperon,I.C., Makarova,O.V., Mayeda,A., Munroe,S.H., Caceres,J.F., Hayward,D.G. and Krainer,A.R. (2000) Selection of alternative 5′ splice sites: Role of U1 snRNP and models for the antagonistic effects of SF2/ASF and hnRNP A1. Mol. Cell. Biol., 20, 8303-8318.
41. Amrein,H., Hedley,M.L. and Maniatis,T. (1994) The role of specific protein-RNA and protein-protein interactions in positive and negative control of pre-mRNA splicing by Transformer 2. Cell, 76, 735-746.
42. Forch,P. and Valcarcel,J. (2003) Splicing regulation in Drosophila sex determination. Prog. Mol. Subcell. Biol., 31, 127-151.
43. Hertel,K.J., Lynch,K.W., Hsiao,E.C., Liu,E.H. and Maniatis,T. (1996) Structural and functional conservation of the Drosophila doublesex splicing enhancer repeat elements. RNA, 2, 969-981.
44. Tian,M. and Maniatis,T. (1994) A splicing enhancer exhibits both constitutive and regulated activities. Genes Dev., 8, 1703-1712.