Design of bifunctional siRNAs: Combining immunostimulation and gene-silencing in one single siRNA molecule

Biochemical and Biophysical Research Communications

Volumn 352, Issue 3, 2007, Pages 642-649

  Furset, Gro ^a   Sioud, Mouldy ^a  

^a OSLO UNIVERSITY HOSPITAL   (Norway)

Author keywords

Dendritic cells; Innate immunity; Interferon pathway; Protein kinase R; RNA interference; siRNAs; Toll like receptors

Indexed keywords

ALPHA INTERFERON; B7 ANTIGEN; CD86 ANTIGEN; CHEMOKINE RECEPTOR CCR7; CYTOKINE; INTERFERON; INTERLEUKIN 10; INTERLEUKIN 12; INTERLEUKIN 6; LIPOSOME; SMALL INTERFERING RNA; TOLL LIKE RECEPTOR; TUMOR NECROSIS FACTOR ALPHA;

ANTIGEN EXPRESSION; ARTICLE; CELL DIFFERENTIATION; CELL MATURATION; CONTROLLED STUDY; CYTOKINE PRODUCTION; DENDRITIC CELL; DRUG DELIVERY SYSTEM; DRUG DESIGN; ELECTROPORATION; GENE EXPRESSION; GENE SILENCING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOSTIMULATION; IN VITRO STUDY; INNATE IMMUNITY; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; RNA SYNTHESIS; T LYMPHOCYTE;

CELLS, CULTURED; GENE SILENCING; GENETIC ENGINEERING; HUMANS; IMMUNIZATION; INTERLEUKIN-10; LEUKOCYTES, MONONUCLEAR; RNA, SMALL INTERFERING;

EID: 33845298010     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2006.11.059     Document Type: Article

References (27)

1. Banchereau J., and Steinman R.M. Dendritic cells and control of immunity. Nature 392 (1998) 245-252
2. Rossi M., and Young J.W. Human dendritic cells: potent antigen-presenting cells at the crossroad of innate and adaptive immunity. J. Immunol. 175 (2005) 1373-1381
3. Takeda K., and Akira S. Toll-like receptors in innate immunity. Int. Immunol. 17 (2005) 1-14
4. Heil F., Hemmi H., Hochrein H., et al. Science 303 (2004) 1526-1529
5. Diebold S.S., Kaisho T., Hemmi H., Akira S., and ReiseSousa C. Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA. Science 303 (2004) 1529-1531
6. Krieg A.M. The role of CpG motifs in innate immunity. Curr. Opin. Immunol. 12 (2000) 35-40
7. Sioud M. Innate sensing of self and non-self RNAs by Toll-like receptors. Trends Mol. Med. 12 (2006) 67-76
8. Grütz G. New insights into the molecular mechanism of interleukin-10-mediated immunosuppression. J. Leukoc. Biol. 77 (2005) 3-15
9. Sioud M. Induction of inflammatory cytokines and interferon responses by double-stranded and single-stranded siRNAs is sequence dependent and requires endosomal localization. J. Mol. Biol. 348 (2005) 1079-1090
10. Hornung V., Guenthner-Biller M., Bourquin C., Ablasser A., Schlee M., Uematsu S., et al. Nature Med. 11 (2005) 63-69
11. Judge A.D., Sood V., Shaw J.R., Fang D., McClintock K., and MacLachlan I. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNAs. Nat. Biotechnol. 23 (2005) 57-62
12. Fire A., Xu S., Montgomery M.K., Kostas S.A., Driver S.E., and Mell C.C. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391 (1998) 806-811
13. Elbashir S.M., Harborth M.J., Ledecknel W., Yalcin A., Weber K., and Tuchl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411 (2001) 494-498
14. Liu J., Carmell M.A., Rivas F.V., Marsden C.G., Thomson J.M., Song J.-J., Hammond S.M., Joshua-Tor L., and Hannon G.J. Argonaute2 is the catalytic engine of mammalian RNAi. Science 305 (2004) 1437-1441
15. Sioud M., and Leirdal M. Potential design rules and enzymatic synthesis of siRNAs. Methods Mol. Biol. 252 (2004) 457-469
16. Sioud M. Single-stranded small interfering RNA are more immunostimulatory than their double-stranded counterparts: a central role for 2'-hydroxyl uridines in immune responses. Eur. J. Immunol. 36 (2006) 1222-1230
17. Trinchieri G. Interleukin-12 and the regulation of innate resistance and adaptive immunity. Nat. Rev. Immunol. 3 (2003) 133-146
18. Kim D.-H., Longo M., Han Y., Lundberg P., Cantin E., and Rossi J.J. Interferon induction by siRNAs and ssRNAs synthesized by phage polymerase. Nat. Biotechnol. 22 (2004) 312-325
19. Yoneyama M., Kikuchi M., Natsukawa T., Shinobu N., Imaizumi T., Miyagishi M., Taira K., Akira S., and Fujita T. The RNA helicase RIG-1 has an essential function in double-stranded RNA-induced innate antiviral responses. Nat. Immunol. 5 (2004) 730-737
20. Takayama T., Morelli A.E., Onai O., Hirao M., Matsuchima K., Tahara H., and Thomson A.W. Mammalian and viral IL10 enhance C-C chemokine receptor 5 but down-regulate C-C chemokine receptor 7 expression by myeloid dendritic cells: impact on chemotactic responses and in vivo homing ability. J. Immunol. 166 (2001) 7136-7143
21. Andre F.E. Vaccinology: past achievements, present roadblocks and future promises. Vaccine 21 (2003) 593-595
22. Wang D., Li Y., Yu D., Song S.S., Kandimalla E.R., and Agrawal S. Immunopharmaco-logical and antitumor effects of second-generation immunomodulatory oligonucleotides containing synthetic CpG motifs. Int. J. Oncol. 24 (2004) 901-908
23. Sparwasser T., Vabulas R.M., Villmow B., Lipford G.B., and Wagner H. Bacterial CpG-DNA activates dendritic cells in vivo: T helper cell-independent cytotoxic T cell responses to soluble proteins. Eur. J. Immunol. 30 (2000) 3591-3597
24. Jones S.A. Directing transition from innate to acquired immunity: defining a role for IL-6. J. Immunol. 175 (2005) 3463-3468
25. Lee J., Chuang T.-H., Redecke V., et al. Molecular basis for the immunostimulatory activity of guanine nucleoside analogs: activation of Toll-like receptor 7. Proc. Natl. Acad. Sci. USA 100 (2003) 6646-6651
26. Doehn C., Kausch I., Melz S., Behm A., and Jocham D. Cytokine and vaccine therapy of Kidney cancer. Expert Rev. Anticancer. Ther. 4 (2004) 1097-1111
27. L. Cekaite, G. Furset, E. Hovig, M. Sioud, Gene expression analysis in blood cells in response to unmodified and 2'-modified siRNAs reveals TLR-dependent and independent effects, J. Mol. Biol., doi:10.1016/j.jb.2006.09.034.