Modulation of the catalytic properties of multimeric β-galactosidase from E. coli by using different immobilization protocols

Enzyme and Microbial Technology

Volumn 40, Issue 2, 2007, Pages 310-315

  Pessela, Benevides C C ^a   Dellamora Ortiz, Gisella ^a   Betancor, Lorena ^a   Fuentes, Manuel ^a   Guisán, Jose M ^a   Fernandez Lafuente, Roberto ^a  

^a INSTITUTO DE CATÁLISIS Y PETROLEOQUÍMICA   (Spain)

Author keywords

Modulation via immobilization; Multimeric enzymes; Oligosaccharides; Transglycosylation

Indexed keywords

ALDEHYDES; CROSSLINKING; ENZYME IMMOBILIZATION; ESCHERICHIA COLI; HYDROLYSIS; PH EFFECTS;

MODULATION VIA IMMOBILIZATION; MULTIMERIC ENZYMES; OLIGOSACCHARIDES; TRANSGLYCOSYLATION;

ENZYMES;

BETA GALACTOSIDASE; EPOXIDE; GLUTARALDEHYDE;

ARTICLE; CONTROLLED STUDY; CROSS LINKING; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME MECHANISM; ESCHERICHIA COLI; HYDROLYSIS; NONHUMAN; TEMPERATURE;

EID: 33751165902     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2006.04.015     Document Type: Article

References (41)

1. Engasser J., and Horvath C. Diffusion and kinetics with immobilized enzymes. In: Wingard L.B., Katctchaski-Katzir E., and Goldstein L. (Eds). Immobilized Enzymes Principles (1976), Academic Press, New York 127-220
2. Buchholz K. Reaction engineering parameters for immobilized biocatalysts. In: Fiechter A. (Ed). Advances Biochemical Engineering, vol. 24 (1982), Springer-Verlag, Berlin
3. Chibata I., Tosa T., and Sato T. Biocatalysis: immobilized cells and enzymes. J Mol Catal 37 (1986) 1-24
4. Gupta M.N. Thermostabilization of proteins. Biotechnol Appl Biochem 14 (1991) 1-11
5. Immobilization of enzymes and cells. In: Guisan J.M. (Ed). G. Series Methods Biotechnology, vol. 22. 2nd ed. (2006), The Humana Press Inc, Totowa
6. Fernández-Lafuente R., Hernández-Jústiz O., Mateo C., Fernández-Lorente G., Terreni M., Alonso J., et al. Biotransformations catalyzed by multimeric enzymes: stabilization of tetrameric ampicillin acylase permits the optimization of ampicillin synthesis under dissociation conditions. Biomacromolecules 2 (2001) 95-104
7. Betancor L., Hidalgo A., Fernández-Lorente G., Mateo C., Fernández-Lafuente R., and Guisan J.M. Preparation of a stable biocatalyst of bovine liver catalase using immobilization and postimmobilization techniques. Biotechnol Prog 19 (2003) 763-767
8. Pessela B.C.C., Mateo C., Fuentes M., Vian A., García J.L., Carrascosa A.V., et al. Stabilization of a multimeric beta-galactosidase from Thermus sp. strain T2 by immobilization on novel heterofunctional epoxy supports plus aldehyde-dextrane cross-linking. Biotechnol Prog 20 (2004) 388-392
9. Pessela B.C.C., Mateo C., Fuentes M., Vian A., García J.L., Carrascosa A.V., et al. The immobilization of a thermophilic beta-galactosidase on Sepabeads supports promotes the reduction of product inhibition. Full enzymatic hydrolysis of lactose in dairy products. Enzyme Microb Technol 33 (2003) 199-205
10. Mateo C., Monti R., Pessela B.C.C., Fuentes M., Torres R., Guisan J.M., et al. Immobilization of lactase from Kluyveromyces lactis greatly reduces the inhibition promoted by glucose. Full hydrolysis of lactose in milk. Biotechnol Prog 20 (2004) 1259-1262
11. Palomo J.M., Fernandez-Lorente G., Ortiz C., Segura R.L., Mateo C., Fuentes M., et al. Improving the resolution of chiral drugs via "conformational engineering of lipases". The effect of directed immobilization and medium engineering. Med Chem Rev 2 (2005) 369-378 [On line]
12. Fernández-Lorente G., Palomo J.M., Mateo C., Guisán J.M., and Fernández-Lafuente R. Resolution of a paroxetine precursor using different lipases. Influence of the reaction conditions on the enantioselectivity of lipases. Enzyme Microb Technol 34 (2004) 264-269
13. Palomo J.M., Fernández-Lorente G., Mateo C., Fernández-Lafuente R., and Guisan J.M. Modulation of the enantioselectivity of the lipase from Candida antarctica B via conformational engineering. Resolution of α-hydroxy-phenylacetic acid derivatives. Tetrahedron Asym 13 (2002) 1337-1345
14. Palomo J.M., Ortiz C., Fernandez-Lorente G., Fuentes M., Guisan J.M., and Fernandez-Lafuente R. Lipase-lipase interactions as a new tool to immobilize and modulate the lipase properties. Enzyme Microb Technol 36 (2005) 445-452
15. Palomo J.M., Segura R.L., Mateo C., Terreni M., Cortes A., Guisan J.M., et al. Synthesis of enantiomerically pure glycidol via a fully enantioselective lipase-catalyzed resolution. Tetrahedron Asym 16 (2005) 869-874
16. Terreni M., Tchamkam J.G., Pagani G., Sarnataro U., Fernandez-Lafuente R., and Guisan J.M. Influence of substrate structure on PGA-catalyzed acylations. Evaluation of different approaches for the enzymatic synthesis of cefonicid. Adv Synth Cat 347 (2005) 121-128
17. Randall L.L., Hardy S., Topping T.B., Smith V.F., Bruce J.E., and Smith R.D. The interaction between the chaperone SecB and its ligands: evidence for multiple subsites for binding. Protein Sci 7 (1998) 2384-2390
18. Sternberg M.J.E., and Thornton J.M. On the conformation of proteins: the handedness of the beta-strand-alpha-helix-beta-strand unit. J Mol Biol 105 (1976) 367-382
19. Jacobson R.H., Zhang X.J., DuBose R.F., and Matthews B.W. Three-dimensional structure of beta-galactosidase from E. coli. Nature 369 (1994) 761-766
20. Mahoney R.R. Galactosyl-oligosaccharide formation during lactose hydrolysis: a review. Food Chem 63 (1998) 147-154
21. Krishina Rao J.V., Jagath J.R., Sharme B., Appagi Rao N., and Sarithri H.S. Asp-89: a critical residue in maintaining the oligomeric structure of sheep liver cytosolic serine hydroxymethyltransferase. Biochem J 343 (1999) 257-263
22. Aronson M. Transgalactosidation during lactose hydrolysis. Arch Biochem Biophy 39 (1952) 370-378
23. Pazur J.H. The enzymatic conversion of lactose into galactosyl oligosaccharides. Science 117 (1953) 355-356
24. Burvall A., Asp N.-G., and Dahlqvist A. Oligosaccharide formation during hydrolysis of lactose with S. lactis lactase (Maxilact)-Part 3: Digestibility by human intestinal enzymes in vitro. Food Chem 5 (1980) 189-194
25. Tomomatsu H. Health effects of oligosaccharides. Food Technol 48 (1994) 61-65
26. Toba T., Watanabe A., and Adachi S. Allolactose and 6-0-β-galactopyranosyl-d-galactose in commercial yoghurt. J Dairy Sci 65 (1982) 33-38
27. Santiago R., Fernández-Mayoralas A., and García-Junceda E. Enzymatic synthesis of disaccharides by β-galactosidase-catalyzed glycosylation of a glycocluster. J Mol Catal B: Enzym 11 (2000) 71-79
28. Fernández-Lafuente R., Rosell C.M., Rodriguez V., Santana C., Soler G., Bastida A., et al. Preparation of activated supports containing low pK amino groups. A new tool for protein immobilisation via the carboxyl coupling method. Enzyme Microb Technol 15 (1993) 546-550
29. Guisán J.M. Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes. Enzyme Microb Technol 10 (1988) 375-382
30. Mateo C., Abian O., Fernández-Lafuente R., and Guisan J.M. Reversible enzyme immobilization via a very strong and non-distorting adsorption on supports-polyethyleneimine composites. Biotechnol Bioeng 68 (2000) 98-105
31. Bradford M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72 (1976) 248-254
32. Mateo C., Abian O., Bernedo M., Cuenca E., Fuentes M., Fernandez-Lorente G., et al. Some special features of glyoxyl supports to immobilize proteins. Enzyme Microb Technol 37 (2005) 456-462
33. Grazu V., Betancor L., Montes T., Lopez-Gallego F., Guisan J.M., and Fernandez-Lafuente R. Glyoxyl-agarose as a new chromatographic matrix. Enzyme Microb Technol 38 (2006) 960-966
34. Mateo C., Palomo J.M., Fuentes M., Betancor L., Grazu V., Lopez-Gallego F., et al. Glyoxyl-agarose: a fully inert hydrophilic support for immobilization and high stabilization of proteins. Enzyme Microb Technol 39 (2006) 274-280
35. Blanco R.M., and Guisan J.M. Stabilization of enzymes by multipoint covalent attachment to agarose-aldehyde gels. Borohydride reduction of trypsin derivatives. Enzyme Microb Technol 11 (1989) 360-366
36. Mateo C., Abian O., Fernández-Lorente G., Predoche J., Fernández-Lafuente R., and Guisan J.M. Sepabeads: a novel epoxy-support for stabilization of industrial enzymes via very intense multipoint covalent attachment. Biotechnol Prog 18 (2002) 629-634
37. Betancor L., López-Gallego F., Hidalgo A., Alonso-Morales N., Dellamora-Ortiz G., Mateo C., et al. Different mechanisms of protein immobilization on glutaraldehyde activated supports: effect of support activation and immobilization conditions. Enzyme Microb Technol 39 (2006) 877-882
38. López-Gallego F., Betancor L., Hidalgo A., Alonso N., Fernandez-Lorente G., Guisan J.M., et al. Preparation of a robust biocatalyst of d-amino acid oxidase on sepabeads supports using the glutaraldehyde crosslinking method. Enzyme Microb Technol 37 (2005) 750-756
39. López-Gallego F., Betancor L., Mateo C., Hidalgo A., Alonso-Morales N., Dellamora-Ortiz G., et al. Enzyme stabilization by glutaraldehyde crosslinking of adsorbed proteins on aminated supports. J Biotechnol 119 (2002) 70-75
40. Alonso N., López-Gallego F., Betancor L., Hidalgo A., Mateo C., Guisan J.M., et al. Immobilization and stabilization of glutaryl acylase on aminated sepabeads supports by the glutaraldehyde crosslinking method. J Mol Catal B: Enzym 35 (2005) 57-61
41. Penzol G., Armisen P., Fernández-Lafuente R., Rodes L., and Guisán J.M. Use of dextrans as long, inert and hydrophilic spacer arms to improve the performance of immobilized proteins acting on macromolecules. Biotechnol Bioeng 60 (1998) 518-523