Biotransformations catalyzed by multimeric enzymes: Stabilization of tetrameric ampicillin acylase permits the optimization of ampicillin synthesis under dissociation conditions

Biomacromolecules

Volumn 2, Issue 1, 2001, Pages 95-104

  Fernandez Lafuente R ^a   Hernandez Justiz O ^a   Mateo, C ^a   Terreni, M ^a   Fernandez Lorente G ^a   Moreno, M A ^c   Alonso, J ^b   Garcia Lopez J L ^b   Guisan, J M ^a  

^a CSIC   (Spain)

^b CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

^c Avenida de Antibioóticos 59 61   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

6 AMINOPENICILLANIC ACID; ALDEHYDE; AMIDASE; AMINO ACID DERIVATIVE; AMPICILLIN; DEXTRAN; ESTER DERIVATIVE; HYDROLASE; METHANOL; PHENYLGLYCINE; PHOSPHATE; TETRAMER; AMPICILLIN ACYLASE; DRUG DERIVATIVE; GLYCINE; IMMOBILIZED ENZYME; METHYL PHENYLGLYCINE; PENICILLANIC ACID; PENICILLIN AMIDASE;

ACETOBACTER; ACYLATION; ARTICLE; CATALYSIS; CHEMICAL REACTION; CONTROLLED STUDY; CROSS LINKING; DISSOCIATION; DRUG STRUCTURE; DRUG SYNTHESIS; DRUG TRANSFORMATION; ENZYME IMMOBILIZATION; ENZYME STABILITY; ENZYME STRUCTURE; MACROMOLECULE; MOLECULAR MODEL; PRIORITY JOURNAL; BIOREACTOR; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; KINETICS; METABOLISM; PH; PROTEIN QUATERNARY STRUCTURE; TEMPERATURE;

ACETOBACTER; ACETOBACTER PASTEURIANUS;

ACETOBACTER; AMPICILLIN; BIOREACTORS; CATALYSIS; ENZYME STABILITY; ENZYMES, IMMOBILIZED; ESCHERICHIA COLI; GLYCINE; HYDROGEN-ION CONCENTRATION; KINETICS; METHANOL; MOLECULAR STRUCTURE; PENICILLANIC ACID; PENICILLIN AMIDASE; PHOSPHATES; PROTEIN STRUCTURE, QUATERNARY; SUPPORT, NON-U.S. GOV'T; TEMPERATURE;

EID: 0034884338     PISSN: 15257797     EISSN: None     Source Type: Journal    
DOI: 10.1021/bm000072i     Document Type: Article

References (53)

1. Enzymes in synthetic organic chemistry
2. Thermostabilization of proteins
3. Enzyme stabilization: State of the art
4. Stabilization of enzymes against thermal inactivation
5. Stability and Stabilization of Biocatalysts
6. Dissociative thermal inactivation, stability and activity of oligomeric enzyme
7. Enzymatic biosynthesis of cephalexin
8. Semisynthetic lactam antibiotics synthesizing enzyme from Acetobacter turbidans: Purification and properties
9. Evaluation of different enzymes as catalysts of the kinetically controlled synthesis of several lactamic antibiotics
10. Mechanism and yields in enzyme catalysed equilibrium and kinetically controlled synthesis of beta-lactamic antibiotics, peptides and other condensation products
11. The use of stabilised penicillin G acylase derivatives improves the design of kinetically controlled synthesis
12. The presence of methanol exerts a strong and complex modulation of the synthesis of different antibiotics by immobilized penicillin G acylase
13. Enzymatic conversions used in the production of penicillins and cephalosporins. Biotechnology of industrial antibiotics
14. One pot chemoenzymatic synthesis of 3′-functionalized cephalosporines (cephazolin) by three consecutive biotransformations in fully aqueous medium
15. Strategies for the stabilization of multimeric enzymes via immobilization and post-immobilization techniques
16. Preparation of activated supports containing low pK amino groups. A new tool for protein immobilization via the carboxyl coupling method
17. Stabilization of immobilized enzymes by chemical modification with polyfunctional macromolecules
18. Immobilization-stabilization of penicillin G acylase from E. coli
19. The coimmobilization of D-amino acid oxidase and catalase enables the quantitative transformation of D-amino acids (phenylalanine) into α-ceto acids (phenylpyruvic acid)
20. Immobilization of enzymes acting on macromolecular substrates: Reduction of steric problems
21. A single step purification, immobilization and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports
22. Immobilization-stabilization of enzymes; variables that control the intensity of the trypsin (amine)-agarose (aldehyde) multipointy attachment
23. Feasibility of the thermodynamically controlled synthesis of amoxicillin
24. Predicting enzyme catalyzed reaction equilibria in cosolvent-water mixtures as a function of pH and solvent composition
25. Thermodynamic controlled synthesis of β-lactam antibiotics. Equilibrium concentrations and side-chain properties
26. Synthesis of antibiotics (cephaloglycin) catalysed by penicillin G Acylase. Evaluation and optimisation of different synthetic approaches
27. Equillibrium controlled synthesis of cephalotin in monophasic water-organic cosolvents systems catalysed by stabilised derivatives of penicillin G acylase
28. Enzyme reaction engineering: Design of the synthesis of antibiotics catalysed by stabilised penicillin G acylase derivatives
29. Dynamic reaction design of enzymatic biotransformations in organic media: Equilibrium controlled synthesis of antibiotics by penicillin G acylase
30. A criterium for the selection of monophasic solvents for enzymatic synthesis
31. Ampicillin and cephalexin synthesis catalyzed by E. coli penicillin amidase. Yield increase due to substrate recycling
32. Enzymatic synthesis of β-lactam antibiotics using penicillin G acylase in frozen media
33. Factors affecting the synthesis of ampicillin and hydroxypenicillins by cell-bound penicillin acylase of Escherichia coli
34. Penicillin acylase-catalyzed synthesis of pivampicillin: Effect of reaction variables and organic solvents
35. Effect of organic solvents on penicillin acylase-catalyzed reactions: Interaction of organic solvents with enzymes
36. Penicillin acylase -catalyzed synthesis of lactam antibiotics in water-methanol mixtures: Effect of cosolvent content and chemical nature of substrate on reaction rates
37. Kinetics and mechanism of acyl transfer by penicillin acylases
38. Kinetic studies on the mechanism of the penicillin amidase-catalyzed synthesis of ampicillin and benzylpenicillin
39. Equilibrium and kinetically controlled synthesis with enzymes: Semisynthesis of penicillins and peptides
40. Modulation of the properties of penicillin G acylase by acyl donor substrates during N-protection of amino compounds
41. Effect of pH in the synthesis of ampicillin acylase
42. Substrate specificity of α-amino acid hydrolase from Xantomonas citri
43. Comparison of three penicillin acylases-type enzymes as hydrolases and acyl tranferase catalysts
44. Reaction kinetics of cephalexin synthesizing enzyme from Xantomonas citri
45. Enzymatic synthesis of aminobenzylpenicillin. Part I. selection of penicillin G acylase-producing bacteria
46. Enzymatic synthesis of D(-)alpha-aminobenzylpenicillin by Kluivera citrophila
47. Semisynthetic lactam antibiotics synthesizing enzyme from Acetobacter turbidans: Catalytic properties
48. Substrate specificity of an alpha-amino acid ester hydrolase produced by Acetobacter turbidams
49. Synthesis of lactam antibiotics containing aminophenylacetyl group in the acyl moiety catalyzed by D-(-) phenylglycyl-β-lactamide aminohydrolase
50. Cephalexin synthesis by partially purified and immobilized enzymes
51. Synthesis of cepahalosporins and penicillins by enzymatic acylation