The interaction between the chaperone SecB and its ligands: Evidence for multiple subsites for binding

Protein Science

Volumn 7, Issue 11, 1998, Pages 2384-2390

  Randall, Linda L ^a   Hardy, Simon J S ^a,c   Topping, Traci B ^a   Smith, Virginia F ^a,d   Bruce, James E ^b   Smith, Richard D ^b  

^a WASHINGTON STATE UNIVERSITY   (United States)

^b PACIFIC NORTHWEST NATIONAL LABORATORY   (United States)

^c UNIVERSITY OF YORK   (United Kingdom)

^d PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

Analytical ultracentrifugation; Calorimetry; Chaperones; Fourier transform ion cyclotron resonance mass spectrometry; SecB

Indexed keywords

CHAPERONE; GALAPTIN; PROTEIN SECB; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL OUTER MEMBRANE; CALORIMETRY; CYCLOTRON; ESCHERICHIA COLI; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN TARGETING; TITRIMETRY; ULTRACENTRIFUGATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0031734169     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560071115     Document Type: Article

References (27)

1. Alexandrescu AT, Abeygunawardana C, Shortle D. 1994. Structure and dynamics of a denatured 131-residue fragment of staphylococcal nuclease: A heteronuclear NMR study. Biochemistry 35:1063-1072.
2. Anderson GA, Bruce JE. 1998. ICR-2LS. Richland, WA: Pacific Northwest National Laboratory. (Available via FTP.)
3. Bruce JE, Smith VF, Liu C, Randall LL, Smith RD. 1998. The observation of chaperone-ligand noncovalent complexes with electrospray ionisation mass spectrometry. Protein Sci 7:1180-1185.
4. Bruce JE, Van Orden SL, Anderson GA, Hofstadler SA, Sherman MG, Rockwood AL, Smith RD. 1995. Selected ion accumulation of noncovalent complexes in a Fourier transform ion cyclotron resonance mass spectrometer. J Mass Spectrom 30:124-133.
5. Collier DN. 1993. SecB: A molecular chaperone of Escherichia coli protein secretion pathway. Adv Protein Chem 44:151-193.
6. Fekkes P, den Blaauwen T, Driessen AJM. 1995. Diffusion-limited interaction between unfolded polypeptides and the Escherichia coli chaperone SecB. Biochemistry 34:10078-10085.
7. Hardy SJS, Randall LL. 1993. Recognition of ligands by SecB, a molecular chaperone involved in bacterial protein export. Philos Trans R Soc Lond B Biol Sci 339:343-352.
8. Hardy SJS, Randall LL. 1991. A kinetic partitioning model of selective binding of nonnative proteins by the bacterial chaperone SecB. Science 251-439-443.
9. Khisty VJ, Munske GR, Randall LL. 1995. Mapping of the binding frame for the chaperone SecB within a natural ligand, galactose-binding protein. J Biol Chem 270:25920-25927.
10. Kumamoto CA. 1991. Molecular chaperones and protein translocation across the Escherichia coli inner membrane. Mol Microbiol 5:19-22.
11. Light-Wahl KJ, Schwartz BL, Smith RD. 1994. Observation of the noncovalent quaternary associations of proteins by electrospray ionization mass spectrometry. J Am Chem Soc 116:5271-5278.
12. Liu CM, Wu Q, Harms AC, Smith RD. 1996. Microdialysis sample clean-up for electrospray ionization-mass spectrometry of nucleic acid samples. Anal Chem 65:3795-3799.
13. Pasa-Tolic L, Bruce JE, Lei QP, Anderson GA, Smith RD. 1998. In-trap cleanup of proteins from electrospray ionization using soft sustained off-resonance irradiation with Fourier transform ion cyclotron resonance mass spectrometry. Anal Chem 70:405-408.
14. Randall LL. 1992. Peptide binding by chaperone SecB: Implications for recognition of non-native structure. Science 257:241-245.
15. Randall LL, Hardy SJS. 1995. High selectivity with low specificity: How SecB solved the paradox of chaperone binding. Trends Biochem Sci 20:65-69.
16. Randall LL, Topping TB, Hardy SJS. 1990. No specific recognition of leader peptide by SecB, a chaperone involved in protein export. Science 248:860-863.
17. Randall LL, Topping TB, Suciu D, Hardy SHS. 1998. Calorimetric analysis of the interaction between SecB and its ligands. Protein Sci 7:1195-1200.
18. Rockwood AL, Van Orden SL. 1996. Ultrahigh-speed calculation of isotope distributions. Anal Chem 68:2027-2030.
19. Shortle D, Meeker AK. 1989. Residual structure in large fragments of staphylococcal nuclease: Effects of amino acid substitutions. Biochemistry 28:936-944.
20. Smith VF, Hardy SJS, Randall LL. 1997. Determination of the binding frame of the chaperone SecB within the physiological ligand oligopeptide-binding protein. Protein Sci 6:1746-1755.
21. Smith VF, Schwartz BL, Randall LL, Smith RD. 1996. Electrospray mass spectrometric investigation of the chaperone SecB. Protein Sci 5:488-494.
22. Stenberg G, Fersht AR. 1997. Folding of barnase in the presence of the molecular chaperone SecB. J Mol Biol 274:268-275.
23. Topping TB, Randall LL. 1994. Determination of the binding frame within a physiological ligand for the chaperone SecB. Protein Sci 3:730-736.
24. Topping TB, Randall LL. 1997. Chaperone SecB from Escherichia coli mediates kinetic partitioning via a dynamic equilibrium with its ligands. J Biol Chem 272:19314-19318.
25. van Holde KE, Weischet WO. 1978. Boundary analysis of sedimentation-velocity experiments with monodisperse and paucidisperse. Biopolymers 17:1387-1403.
26. Winger BE, Hofstadler SA, Bruce JE, Udseth HR, Smith RD. 1993. High resolution accurate mass measurements of biomolecules using a new electrospray ionization ion cyclotron resonance mass spectrometer. J Am Soc Mass Spectrom 4:566-577.
27. Wiseman T, Williston S, Brandts JF, Lin L-N. 1989. Rapid measurement of binding constants and heats of binding using a new titration calorimeter. Anal Biochem 179:131-137.