Stabilization of a Multimeric β-Galactosidase from Thermus sp. Strain T2 by Immobilization on Novel Heterofunctional Epoxy Supports Plus Aldehyde-Dextran Cross-Linking

Biotechnology Progress

Volumn 20, Issue 1, 2004, Pages 388-392

  Pessela, Benevides C C ^a   Mateo, Cesar ^a   Fuentes, Manuel ^a   Vian, Alejandro ^a   García, José L ^b   Carrascosa, Alfonso V ^a   Guisán, José M ^a   Fernández Lafuente, Roberto ^a  

^a UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

^b Campus Universidad Autónoma   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCATALYSTS; CONTAMINATION; CROSSLINKING; ENZYMES;

ALKALINE INCUBATION; FOOD TECHNOLOGY;

BIOTECHNOLOGY;

ALDEHYDE; BETA GALACTOSIDASE; DEXTRAN; EPOXIDE; IMMOBILIZED ENZYME; LACTOSE; POLYMER;

ADSORPTION; ARTICLE; CHEMISTRY; CLASSIFICATION; DIMERIZATION; ENZYME ACTIVATION; ENZYME STABILITY; ENZYMOLOGY; HYDROLYSIS; PROTEIN CONFORMATION; PROTEIN QUATERNARY STRUCTURE; SPECIES DIFFERENCE; THERMUS;

ADSORPTION; ALDEHYDES; BETA-GALACTOSIDASE; DEXTRANS; DIMERIZATION; ENZYME ACTIVATION; ENZYME STABILITY; ENZYMES, IMMOBILIZED; EPOXY COMPOUNDS; HYDROLYSIS; LACTOSE; POLYMERS; PROTEIN CONFORMATION; PROTEIN STRUCTURE, QUATERNARY; SPECIES SPECIFICITY; THERMUS;

THERMUS; THERMUS SP.;

EID: 1142269586     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp034183f     Document Type: Article

References (14)

1. Bastida, A.; Sabuquillo, P.; Armisén, P.; Fernádez- Lafuente, R.; Huguet, J.; Guisan, J. M. A single step purification, immobilization and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports. Biotechnol. Bioeng. 1998, 58, 486-493.
2. Bradford, M. M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 68, 98-105.
3. Fernández-Lafuente, R.; Hernández-Jústiz, O.; Mateo, C.; Fernández-Lorente, G.; Terrini, M.; Alonso, J.; García- López, J. L.; Moreno, M. A.; Guisán, J. M. Biotransformations catalysed by multimeric enzymes: stabilization of tetrameric ampicillin acylase permits the optimization of ampicillin synthesis under dissociation conditions. Biomacromolecules 2001, 2, 95-104.
4. Fernández-Lafuente, R.; Rodríguez, V.; Mateo, C.; Penzol, G.; Hernández-Jústiz, O.; Irazoqui, G.; Villarino, A.; Ovsejevi, K.; Batista, F.; Guisán, J. M. Strategies for the stabilization of multimeric enzymes via immobilization and post-immobilization techniques. J. Mol. Catal. B: Enzym. 1999, 7, 181-189.
5. Guisán, J. M.; Penzol, G.; Armisén, P.; Bastida, A.; Blanco, R. M.; Fernández-Lafuente, R.; García-Junceda, E. Immobilization of enzymes acting on macromolecular substrates: reduction of steric problems. In Methods in Biotechnology 1, Immobilization of Enzymes and Cells; Bickerstaff, G. F., Ed.; Humana Press: Totowa, NJ, 1997; pp 261-276.
6. Katchalski-Katzir, E.; Kraemer, D. Eupergit C, a carrier for immobilization of enzymes of industrial potential. J. Mol. Catal. B: Enzym. 2000, 10, 157-176.
7. Kramer, D. M.; Lehmann, K.; Pennewiss, H.; Plainer, H. Oxirane acrylic beads for protein immobilization: A novel matrix for biocatalysts and biospecific adsorption. 26th International IUPAC Symposium on Macromolecules, 1979.
8. Mateo, C.; Fernández-Lorente, G.; Cortés, E.; García, J. L.; Fernández-Lafuente, R.; Guisán, J. M. One-step purification, covalent immobilization, and additional stabilization of poly-His-tagged proteins using novel heterofunctional chelate-epoxy supports. Biotechnol. Bioeng. 2001, 76, 269-277.
9. Mateo, C.; Abián, O.; Fernández-Lafuente, R.; Guisán, J. M. Increasing in conformational stability of enzymes immobilized on epoxy-activated supports by favouring additional multi-point covalent attachment. Enzyme Microbiol. Technol. 2000a, 26, 509-515.
10. Mateo, C.; Abián, O.; Fernández-Lorente, G.; Pedroche, J.; Fernández-Lafuente, R.; Tam, A.; Daminati, M.; Guisán, J. M. Epoxy sepabeads: A novel epoxy support for stabilization of industrial enzymes via very intense multipoint covalent attachment. Biotechnol. Prog. 2002, 18, 629-634.
11. Mateo, C.; Fernández-Lorente, G.; Abián, O.; Fernández-Lafuente, R.; Guisán, J. M. Multifunctional epoxy supports: A new tool to improve the immobilization of proteins. The promotion of physical adsorption of proteins on the supports before their covalent linkage. Biomacromolecules 2000b, 1, 739-745.
12. Pessela, B. C. Ch.; Vian, A.; Mateo, C.; Fernández-Lafuente, R.; García, J. L.; Guisán, J. M.; Carrascosa, A. V. Over production of Thermus sp. T2 beta-galactosidase in Escherichia coli and preparation using Taylor-made metal chelate supports. Appl. Envion. Microb. 2003, 69, 1967-1972.
13. Pessela, B. C. Ch.; Vián, A.; Mateo, C.; Fernández- Lafuente, R.; García, J. L.; Guisán, J. M.; Carrascosa, A. V. Hidrólisis de lactosa con lactasa thermorresistente inmovilizada y su método de purificación. Spanish patent application 200200419, 2002.
14. Wheatley, J. B.; Schmidt, D. E. Salt-induced immobilization of affinity ligands onto epoxide-activated supports. J. Chromatogr. A 1999, 849, 1-12.