Leishmania tarentolae: Purification and characterization of tubulin and its suitability for antileishmanial drug screening

Experimental Parasitology

Volumn 114, Issue 4, 2006, Pages 289-296

  Yakovich, Adam J ^a   Ragone, Frank L ^a   Alfonzo, Juan D ^a   Sackett, Dan L ^b   Werbovetz, Karl A ^a  

^a OHIO STATE UNIVERSITY   (United States)

^b EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

Author keywords

buffer consisting of 0.1 M Pipes (pH 6.9), 1 mM MgCl2, and 1 mM EGTA; DEAE; diethylaminoethyl; dimethyl sulfoxide; Dinitroaniline; DMSO; Drug discovery; EGTA; ethyleneglycol bis( aminoethyl ether); GB II 150; GB II 5; Leishmania; N1 phenyl 3,5 dinitro N4,N4 di n butylsulfanilamide; N1 phenyl 3,5 dinitro N4,N4 di n propylsulfanilamide; PBS; phosphate buffered saline; PME; Tubulin

Indexed keywords

ALPHA TUBULIN; ANILINE DERIVATIVE; ANTILEISHMANIAL AGENT; BETA TUBULIN; CELL PROTEIN; N1 PHENYL 3,5 DINITRO N4,N4 DI N BUTYLSULFANILAMIDE; N1 PHENYL 3,5 DINITRO N4,N4 DI N PROPYLSULFANILAMIDE; ORYZALIN; SEPHAROSE; SULFANILAMIDE DERIVATIVE; TUBULIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BINDING AFFINITY; CHROMATOGRAPHY; CONTROLLED STUDY; DRUG INHIBITION; DRUG SCREENING; DRUG SENSITIVITY; LEISHMANIA; LEISHMANIA MAJOR; LEISHMANIA TARENTOLAE; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN BINDING; PROTEIN PURIFICATION; SPECIES COMPARISON; ULTRASOUND;

AMINO ACID SEQUENCE; ANIMALS; CHROMATOGRAPHY, AGAROSE; DINITROBENZENES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; LEISHMANIA; LIGANDS; MOLECULAR SEQUENCE DATA; SEQUENCE ALIGNMENT; SONICATION; SULFANILAMIDES; TUBULIN; TUBULIN MODULATORS;

LEISHMANIA AMAZONENSIS; LEISHMANIA MAJOR; LEISHMANIA TARENTOLAE;

EID: 33750333141     PISSN: 00144894     EISSN: 10902449     Source Type: Journal    
DOI: 10.1016/j.exppara.2006.04.008     Document Type: Article

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