Pregnenolone sulphate and Zn2+ inhibit recombinant rat GABA A receptor through different channel property

Acta Physiologica

Volumn 188, Issue 3-4, 2006, Pages 153-162

  Wang, M D ^a   Rahman, M ^a   Zhu, D ^a   Backstrom T ^a  

^a UMEÅ UNIVERSITY   (Sweden)

Author keywords

GABA; GABAA receptor; Pentobarbital; Pregnenolone sulphate; Zn 2+

Indexed keywords

4 AMINOBUTYRIC ACID; 4 AMINOBUTYRIC ACID A RECEPTOR; 4 AMINOBUTYRIC ACID A RECEPTOR BLOCKING AGENT; CHLORIDE CHANNEL; CHLORIDE CHANNEL BLOCKING AGENT; PENTOBARBITAL; PICROTOXIN; PREGNENOLONE SULFATE; RECEPTOR SUBUNIT; ZINC ION; 4 AMINOBUTYRIC ACID RECEPTOR BLOCKING AGENT; BENZODIAZEPINE RECEPTOR AFFECTING AGENT; PREGNENOLONE; RECOMBINANT PROTEIN; ZINC;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CHANNEL GATING; CONTROLLED STUDY; CYTOPLASM; FEMALE; NONHUMAN; OOCYTE; PRIORITY JOURNAL; STATISTICAL SIGNIFICANCE; VOLTAGE CLAMP; WILD TYPE; XENOPUS; ANIMAL; DOSE RESPONSE; DRUG ANTAGONISM; GENETICS; METABOLISM; METHODOLOGY; MUTATION; PATCH CLAMP; RAT; XENOPUS LAEVIS;

ANIMALS; CHLORIDE CHANNELS; DOSE-RESPONSE RELATIONSHIP, DRUG; FEMALE; GABA ANTAGONISTS; GABA MODULATORS; MUTATION; OOCYTES; PATCH-CLAMP TECHNIQUES; PENTOBARBITAL; PICROTOXIN; PREGNENOLONE; RATS; RECEPTORS, GABA-A; RECOMBINANT PROTEINS; XENOPUS LAEVIS; ZINC;

EID: 33750311821     PISSN: 17481708     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1748-1716.2006.01617.x     Document Type: Article

References (39)

1. Akaike, N., Inoue, M. & Krishtal, O.A. 1986. 'Concentrationclamp' study of gamma-aminobutyric-acid-induced chloride current kinetics in frog sensory neurones. J Physiol 379, 171-185.
2. Akk, G., Bracamontes, J. & Steinbach, J.H. 2001. Pregnenolone sulfate block of GABA(A) receptors: mechanism and involvement of a residue in the M2 region of the alpha subunit. J Physiol 532, 673-684.
3. Barberis, A., Petrini, E.M., Cherubini, E. & Mozrzymas, J.W. 2002. Allosteric interaction of zinc with recombinant alpha(1)beta(2)gamma(2) and alpha(1)beta(2) GABA(A) receptors. Neuropharmacology 43, 607-618.
4. Buhl, E.H., Otis, T.S. & Mody, I. 1996. Zinc-induced collapse of augmented inhibition by GABA in a temporal lobe epilepsy model. Science 271, 369-373.
5. Chang, Y., Wang, R., Barot, S. & Weiss, D.S. 1996. Stoichiometry of a recombinant GABAA receptor. J Neurosci 16, 5415-5424.
6. Corpechot, C., Collins, B.E., Carey, M.P., Tsouros, A., Robel, P. & Fry, J.P. 1997. Brain neurosteroids during the mouse oestrous cycle. Brain Res 766, 276-280.
7. A receptor subtypes by Zn2+. Neuron 5, 781-788.
8. A receptor-mediated current. J Physiol 550, 679-691.
9. England, P.M., Zhang, Y., Dougherty, D.A. & Lester, H.A. 1999. Backbone mutations in transmembrane domains of a ligand-gated ion channel: implications for the mechanism of gating. Cell 96, 89-98.
10. Farrar, S.J., Whiting, P.J., Bonnert, T.P. & McKernan, R.M. 1999. Stoichiometry of a ligand-gated ion channel determined by fluorescence energy transfer. J Biol Chem 274, 10100-10104.
11. Frederickson, C.J. 1989. Neurobiology of zinc and zinc-containing neurons. Int Rev Neurobiol 31, 145-238.
12. Gibbs, J.W., III, Shumate, M.D. & Coulter, D.A. 1997. Differential epilepsy-associated alterations in postsynaptic GABA(A) receptor function in dentate granule and CA1 neurons. J Neurophysiol 77, 1924-1938.
13. Gingrich, K.J. & Burkat, P.M. 1998. Zn2+ inhibition of recombinant GABAA receptors: an allosteric, state-dependent mechanism determined by the gamma-subunit. J Physiol 506, 609-625.
14. Haage, D., Backstrom, T. & Johansson, S. 2005. Interaction between allopregnanolone and pregnenolone sulfate in modulating GABA-mediated synaptic currents in neurons from the rat medial preoptic nucleus. Brain Res 1033, 58-67.
15. Horenstein, J., Wagner, D.A., Czajkowski, C. & Akabas, M.H. 2001. Protein mobility and GABA-induced conformational changes in GABA(A) receptor pore-lining M2 segment. Nat Neurosci 4, 477-485.
16. Hoshino, K., Kondo, J., Soma, T., Kurata, H., Imoto, K. & Matsumoto, A. 1986. Rupture of the right pulmonary artery aneurysm after shunt operation of tetralogy of Fallot. Nippon Kyobu Geka Gakkai Zasshi 34, 1839-1843.
17. Hosie, A.M., Dunne, E.L., Harvey, R.J. & Smart, T.G. 2003. Zinc-mediated inhibition of GABA(A) receptors: discrete binding sites underlie subtype specificity. Nat Neurosci 6, 362-369.
18. Imoto, K., Methfessel, C., Sakmann, B., Mishina, M., Mori, Y., Konno, T., Fukuda, K., Kurasaki, M., Bujo, H., Fujita, Y. et al. 1986. Location of a delta-subunit region determining ion transport through the acetylcholine receptor channel. Nature 324, 670-674.
19. A receptors. Neuropharmacology 35, 1289-1298.
20. 2+ on recombinant and native rat neuronal GABAA receptors. J Physiol 507, 639-652.
21. Lambert, J.D., Fueta, Y., Roepstorff, A. & Andreasen, M. 1996. Analysis of the kinetics of synaptic inhibition points to a reduction in GABA release in area CA1 of the genetically epileptic mouse, El. Epilepsy Res 26, 15-23.
22. Lanthier, A. & Patwardhan, V.V. 1986. Sex steroids and 5-en-3 beta-hydroxysteroids in specific regions of the human brain and cranial nerves. J Steroid Biochem 25, 445-449.
23. Legendre, P. & Westbrook, G.L. 1991. Noncompetitive inhibition of gamma-aminobutyric acidA channels by Zn. Mol Pharmacol 39, 267-274.
24. A receptor-mediated currents via a reduction of channel opening frequency. Brain Res 489, 190-194.
25. Mishina, M., Takai, T., Imoto, K., Noda, M., Takahashi, T., Numa, S., Methfessel, C. & Sakmann, B. et al. 1986. Molecular distinction between fetal and adult forms of muscle acetylcholine receptor. Nature 321, 406-411.
26. Molnar, P. & Nadler, J.V. 2001. Lack of effect of mossy fiber-released zinc on granule cell GABA(A) receptors in the pilocarpine model of epilepsy. J Neurophysiol 85, 1932-1940.
27. Shen, W., Mennerick, S., Zorumski, E.C., Covey, D.F. & Zorumski, C.F. 1999. Pregnenolone sulfate and dehydroepiandrosterone sulfate inhibit GABA-gated chloride currents in Xenopus oocytes expressing picrotoxin- insensitive GABA(A) receptors. Neuropharmacology 38, 267-271.
28. Shen, W., Mennerick, S., Covey, D.F. & Zorumski, C.F. 2000. Pregnenolone sulfate modulates inhibitory synaptic transmission by enhancing GABA(A) receptor desensitization. J Neurosci 20, 3571-3579.
29. Sieghart, W. 1995. Structure and pharmacology of gamma-aminobutyric acidA receptor subtypes. Pharmacol Rev 47, 181-234.
30. A receptor complex in cultured rat neurones. J Physiol 447, 587-625.
31. A-receptor complex. Br J Pharmacol 99, 643-654.
32. Smart, T.G., Constanti, A., Houamed, K., Bilbe, G., Brown, D.A., Barnard, E.A. & Van Renterghem, C. et al. 1986. Expression of vertebrate amino acid receptors in Xenopus oocytes. Adv Exp Med Biol 203, 525-537.
33. A receptors are differentially sensitive to zinc: dependence on subunit composition. Br J Pharmacol 103, 1837-1839.
34. A receptor subtype. J Neurosci 17, 2728-2737.
35. Wang, M.D., Wahlstrom, G. & Backstrom, T. 1997. The regional brain distribution of the neurosteroids pregnenolone and pregnenolone sulfate following intravenous infusion. J Steroid Biochem Mol Biol 62, 299-306.
36. Wang, M., He, Y., Eisenman, L.N., Fields, C., Zeng, C.M., Mathews, J., Benz, A., Fu, T., Zorumski, E., Steinbach, J.H., Covey, D.F., Zorumski, C.F. & Mennerick, S. et al. 2002. 3beta-hydroxypregnane steroids are pregnenolone sulfatelike GABA(A) receptor antagonists. J Neurosci 22, 3366-3375.
37. Westbrook, G.L. & Mayer, M.L. 1987. Micromolar concentrations of Zn2+ antagonize NMDA and GABA responses of hippocampal neurons. Nature 328, 640-643.
38. Xie, X. & Smart, T.G. 1994. Modulation of long-term potentiation in rat hippocampal pyramidal neurons by zinc. Pflugers Arch 427, 481-486.
39. Xie, X., Hider, R.C. & Smart, T.G. 1994. Modulation of GABA-mediated synaptic transmission by endogenous zinc in the immature rat hippocampus in vitro. J Physiol 478, 75-86.