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Volumn 16, Issue 5, 2006, Pages 644-653

Recent advances and applications of site-directed spin labeling

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE PROTEIN; NITROXIDE; RETINA S ANTIGEN; RHODOPSIN;

EID: 33749041288     PISSN: 0959440X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.sbi.2006.08.008     Document Type: Review
Times cited : (282)

References (76)
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    • Li Y., Han X., Lai A.L., Bushweller J.H., Cafiso D.S., and Tamm L.K. Membrane structures of the hemifusion-inducing fusion peptide mutant G1S and the fusion-blocking mutant G1V of influenza virus hemagglutinin suggest a mechanism for pore opening in membrane fusion. J Virol 79 (2005) 12065-12076. This work combines structural data from NMR and depth data from SDSL to determine the structures and positions of fusion peptides, and selected mutants, within the lipid bilayer. It indicates that the shape and position assumed by these peptides is important for their fusion activity.
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    • Kim M., Xu Q., Fanucci G.E., and Cafiso D.S. Solutes modify a conformational transition in a membrane transport protein. Biophys J 90 (2006) 2922-2929. This work shows that a conformational change seen by EPR may be modulated by solution osmolality and that the sensitivity to osmolality is consistent with the structural change observed. This observation provides a likely explanation for the absence of this transition in the protein crystal structure.
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    • An outward tilt of transmembrane helix 6 of rhodopsin accompanies the phototransduction process. This work demonstrates that this structural change takes place in lipid bilayers and in micelles of dodecylmaltoside. It also nicely demonstrates that this structural change and optical changes in the chromophore are not directly coupled. Unlike the optical changes, the structural change is not strongly coupled to bilayer composition.
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    • Qin P.Z., Iseri J., and Oki A. A model system for investigating lineshape/structure correlations in RNA site-directed spin labeling. Biochem Biophys Res Commun 343 (2006) 117-124. EPR spectra of proteins have lineshapes that reflect local protein structure. This work demonstrates that RNA structural elements have unique spectral signatures. The authors establish a procedure to generate a library of EPR spectra for RNA.
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    • This work combines distance data obtained by EPR to define the complex between the chemotactic adaptor protein CheW and the histidine kinase CheA.
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