Measurement of large distances in biomolecules using double-quantum filtered refocused electron spin-echoes

Journal of the American Chemical Society

Volumn 126, Issue 25, 2004, Pages 7746-7747

  Borbat, Peter P ^a   Davis, Jared H ^b   Butcher, Samuel E ^b   Freed, Jack H ^a  

^a Cornell University ^*  (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN; RNA;

ACCURACY; ANALYTIC METHOD; ARTICLE; BASE PAIRING; DIFFUSION; DIPOLE; DOUBLE QUANTUM FILTERED REFOCUSED ELECTRON SPIN ECHO; ELECTRON SPIN RESONANCE; HEATING; MEASUREMENT; OSCILLATION; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN STRUCTURE; QUANTUM MECHANICS; SPIN LABELING; STRUCTURE ANALYSIS; ULTRAVIOLET SPECTROSCOPY;

BASE PAIRING; BIOPHYSICS; CRYSTALLOGRAPHY, X-RAY; ELECTRON SPIN RESONANCE SPECTROSCOPY; MODELS, MOLECULAR; NITRIC OXIDE; QUANTUM THEORY; RNA; THIOURIDINE;

EID: 3042690165     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja049372o     Document Type: Article

References (14)

1. Borbat, P. P.; Freed, J. H. Chem. Phys. Lett. 1999, 313, 145-154.
2. Borbat, P. P.; Freed, J. H. Biol. Magn. Reson. 2001, 19, 383-459.
3. Borbat, P. P.; Mchaourab, H. S.; Freed, J. H. J. Am. Chem. Soc. 2002, 124, 5304-5314.
4. Berliner, L. J., Eaton, G. R., Eaton, S. S., Eds. Distance Measurements in Biological Systems; Biological Magnetic Resonance, Vol. 19; Kluwer Academic: New York, 2000, provides extensive reviews of current ESR methods for distance measurements.
5. Milov, A. D.; Maryasov, A. G.; Tsvetkov, Y. D. Appl. Magn. Reson. 1998, 15, 107-143.
6. Pannier, M.; Veit, S.; Godt, A.; Jeschke, G.; Spiess, H. W. J. Magn. Reson. 2000, 142, 331-340.
7. Huber, M.; Lindgren, M.; Hammarstrom, P.; Martensson, L. G.; Carlsson, U.; Eaton, G. R.; Eaton, S. S. Biophys. Chem. 2001, 94, 245-256.
8. Schiemann, O.; Weber, A.; Edwards, T. E.; Prisner, T. F.; Siguordsson, S. T. J. Am. Chem. Soc. 2003, 125, 3434-3435 have reported a 35 Å distance across an RNA duplex using nitroxide labels and PELDOR.
9. Ramos, A.; Varani, G. J. Am. Chem. Soc. 1998, 120, 10992-10993.
10. Borbat, P. P.; Crepeau, R. H.; Freed, J. H. J. Magn. Reson. 1997, 127, 155-167.
11. Dikanov, S. A.; Tsvetkov, Yu. D. Electron Spin-Echo Envelope Modulation (ESEEM) Spectroscopy; CRC Press: Boca Raton, FL, 1992.
12. Note that for our dilute 100 μM solutions, corresponding to a mean distance of 255 Å between RNAs, instantaneous diffusion was weak and readily corrected for by standard methods. (cf. refs 3, 13)
13. Jeschke, G.; Koch, A.; Jonas, U.; Godt, A. J. Magn. Reson. 2002, 155, 72-82.
14. 4-6 (or DEER) techniques may usefully be applied to distances of 20-50 Å, but conditions of refocusing unfortunately lead to a decrease in the measurable distance range (cf. ref 6).