Spectroscopic Evidence that Osmolytes Used in Crystallization Buffers Inhibit a Conformation Change in a Membrane Protein

Biochemistry

Volumn 42, Issue 45, 2003, Pages 13106-13112

  Fanucci, Gail E ^a   Lee, Jae Y ^a   Cafiso, David S ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

SITE-DIRECTED SPIN LABELING (SDSL);

BACTERIA; BIOLOGICAL MEMBRANES; CRYSTAL STRUCTURE; CRYSTALLIZATION; HYDRATION; POLYETHYLENE GLYCOLS; SPECTROSCOPIC ANALYSIS;

PROTEINS;

CYANOCOBALAMIN; MACROGOL; OUTER MEMBRANE PROTEIN; PROTEN BTUB; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; CRYSTALLIZATION; HYDRATION; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN STRUCTURE; SPECTROSCOPY; SPIN LABELING;

BACTERIAL OUTER MEMBRANE PROTEINS; BUFFERS; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; ELECTRON SPIN RESONANCE SPECTROSCOPY; ESCHERICHIA COLI PROTEINS; MEMBRANE TRANSPORT PROTEINS; OSMOLAR CONCENTRATION; OSMOTIC PRESSURE; PEPTIDE FRAGMENTS; POLYETHYLENE GLYCOLS; PROTEIN CONFORMATION; PROTEIN FOLDING; RECEPTORS, PEPTIDE; SENSITIVITY AND SPECIFICITY; SOLUTIONS; SPIN LABELS;

BACTERIA (MICROORGANISMS);

EID: 0242569215     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi035439t     Document Type: Article

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