Induction of single chain tetracycline repressor requires the binding of two inducers

Nucleic Acids Research

Volumn 34, Issue 14, 2006, Pages 3834-3841

  Kamionka, Annette ^a   Majewski, Marius ^a   Roth, Karin ^a   Bertram, Ralph ^a   Kraft, Christine ^a   Hillen, Wolfgang ^a  

^a UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

4 DEDIMETHYLAMINOANHYDROTETRACYCLINE; ANHYDROTETRACYCLINE; BACTERIAL DNA; DIMER; ESCHERICHIA COLI PROTEIN; MUTANT PROTEIN; PROTEIN SUBUNIT; REPRESSOR PROTEIN; TETRACYCLINE; TETRACYCLINE DERIVATIVE; TETRACYCLINE REPRESSOR PROTEIN; UNCLASSIFIED DRUG; UREA; TETRACYCLINE RESISTANCE ENCODING TRANSPOSON REPRESSOR PROTEIN; TETRACYCLINE RESISTANCE-ENCODING TRANSPOSON REPRESSOR PROTEIN;

ARTICLE; BINDING SITE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA SEQUENCE; ESCHERICHIA COLI; GENETIC STABILITY; GENETIC VARIABILITY; IN VITRO STUDY; IN VIVO STUDY; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DENATURATION; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY; REGULATORY MECHANISM; STOICHIOMETRY; THERMODYNAMICS; TRANSCRIPTION INITIATION; BIOSYNTHESIS; CHEMISTRY; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; MUTATION;

ESCHERICHIA COLI;

BINDING SITES; ESCHERICHIA COLI; MUTATION; PROTEIN DENATURATION; REPRESSOR PROTEINS; TETRACYCLINE; TETRACYCLINES; THERMODYNAMICS; TRANSCRIPTION, GENETIC;

EID: 33748540072     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkl316     Document Type: Article

References (37)

1. Browning,D.F. and Busby,S.J. (2004) The regulation of bacterial transcription initiation. Nature Rev. Microbiol., 2, 57-65.
2. Huffman,J.L. and Brennan,R.G. (2002) Prokaryotic transcription regulators: More than just the helix-turn-helix motif. Curr. Opin. Struct. Biol., 12, 98-106.
3. Zaim,J. and Kierzek,A.M. (2003) The structure of full-length LysR-type transcriptional regulators. Modeling of the full-length OxyR transcription factor dimer. Nucleic Acids Res., 31, 1444-1454.
4. Busby,S. and Ebright,R.H. (1999) Transcription activation by catabolite activator protein (CAP). J Mol. Biol., 293, 199-213.
5. Henssler,E.M., Bertram,R., Wisshak,S. and Hillen,W. (2005) Tet repressor mutants with altered effector binding and allostery. FEBS J., 272, 4487-4496.
6. Scholz,O., Henssler,E.M., Bail,J., Schubert,P., Bogdanska-Urbaniak,J., Sopp,S., Reich,M., Wisshak,S., Köstner,M., Bertram,R. et al. (2004) Activity reversal of Tet repressor caused by single amino acid exchanges. Mol. Microbiol., 53, 777-789.
7. Schnappinger,D., Schubert,P., Pfleiderer,K. and Hillen,W. (1998) Determinants of protein-protein recognition by four helix bundles: changing the dimerization specificity of Tet repressor. EMBO J., 17, 535-543.
8. Schubert,P., Pfleiderer,K. and Hillen,W. (2004) Tet repressor residues indirectly recognizing anhydrotetracycline. Eur. J Biochem., 271, 2144-2152.
9. Orth,P., Schnappinger,D., Hillen,W., Saenger,W. and Hinrichs,W. (2000) Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system [see comments]. Nature Struct. Biol., 7, 215-219.
10. Kedracka-Krok,S., Gorecki,A., Bonarek,P. and Wasylewski,Z. (2005) Kinetic and thermodynamic studies of tet repressor-tetracycline interaction. Biochemistry, 44, 1037-1046.
11. Ehrt,S., Guo,X.V., Hickey,C.M., Ryou,M., Monteleone,M., Riley,L.W. and Schnappinger,D. (2005) Controlling gene expression in mycobacteria with anhydrotetracycline and Tet repressor. Nucleic Acids Res., 33, e21.
12. Gossen,M. and Bujard,H. (2001) Tetracyclines in the control of gene expression in eukaryotes. In Nelson,M., Hillen,W. and Greenwald,R.A. (eds), Tetracyclines in Biology, Chemistry and Medicine. Birkhäuser Verlag, Switzerland, pp. 139-158.
13. Weinmann,P., Gossen,M., Hillen,W., Bujard,H. and Gatz,C. (1994) A chimeric transactivator allows tetracycline-responsive gene expression in whole plants. Plant J., 5, 559-569.
14. Berens,C. and Hillen,W. (2003) Gene regulation by tetracyclines. Constraints of resistance regulation in bacteria shape TetR for application in eukaryotes. Eur. J Biochem., 270, 3109-3121.
15. Kisker,C., Hinrichs,W., Tovar,K., Hillen,W. and Saenger,W. (1995) The complex formed between Tet repressor and tetracycline-Mg2+ reveals mechanism of antibiotic resistance. J Mol. Biol., 247, 260-280.
16. Kamionka,A., Bogdanska-Urbaniak,J., Scholz,O. and Hillen,W. (2004) Two mutations in the tetracycline repressor change the inducer anhydrotetracycline to a corepressor. Nucleic Acids Res., 32, 842-847.
17. Brown,N.L., Stoyanov,J.V., Kidd,S.P. and Hobman,J.L. (2003) The MerR family of transcriptional regulators. FEMS Microbiol. Rev., 27, 145-163.
18. Dieckmann,G.R., McRorie,D.K., Lear,J.D., Sharp,K.A., DeGrado,W.F. and Pecoraro,V.L. (1998) The role of protonation and metal chelation preferences in defining the properties of mercury-binding coiled coils. J. Mol. Biol., 280, 897-912.
19. Shewchuk,L.M., Verdine,G.L., Nash,H. and Walsh,C.T. (1989) Mutagenesis of the cysteines in the metalloregulatory protein MerR indicates that a metal-bridged dimer activates transcription. Biochemistry, 28, 6140-6145.
20. Ramos,J.L., Martinez-Bueno,M., Molina-Henares,A.J., Teran,W., Watanabe,K., Zhang,X., Gallegos,M.T., Brennan,R. and Tobes,R. (2005) The TetR family of transcriptional repressors. Microbiol. Mol. Biol. Rev., 69, 326-356.
21. Schumacher,M.A., Miller,M.C., Grkovic,S., Brown,M.H., Skurray,R.A. and Brennan,R.G. (2001) Structural mechanisms of QacR induction and multidrug recognition. Science, 294, 2158-2163.
22. Krueger,C., Berens,C., Schmidt,A., Schnappinger,D. and Hillen,W. (2003) Single-chain Tet transregulators. Nucleic Acids Res., 31, 3050-3056.
23. Sambrook,J. (2001) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
24. Miller,J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
25. Watt,V.M., Ingles,C.J., Urdea,M.S. and Rutter,W.J. (1985) Homology requirements for recombination in Escherichia coli. Proc. Natl Acad. Sci. USA, 82, 4768-4772.
26. Heinz,C., Karosi,S. and Niederweis,M. (2003) High-level expression of the mycobacterial porin MspA in Escherichia coli and purification of the recombinant protein. J Chromatogr. B. Analyt. Technol. Biomed. Life Sci., 790, 337-348.
27. Kamionka,A., Bertram,R. and Hillen,W. (2005) Tetracycline-dependent conditional gene knockout in Bacillus subtilis. Appl. Environ. Microbiol., 71, 728-733.
28. Ettner,N., Müller,G., Berens,C., Backes,H., Schnappinger,D., Schreppel,T., Pfleiderer,K. and Hillen,W. (1996) Fast large-scale purification of tetracycline repressor variants from overproducing Escherichia coli strains. J. Chromatogr. A., 742, 95-105.
29. Müller,G., Hecht,B., Helbl,V., Hinrichs,W., Saenger,W. and Hillen,W. (1995) Characterization of non-inducible Tet repressor mutants suggests conformational changes necessary for induction. Nature Struct. Biol., 2, 693-703.
30. Scholz,O., Schubert,P., Kintrup,M. and Hillen,W. (2000) Tet repressor induction without Mg2+. Biochemistry, 39, 10914-10920.
31. Henssler,E.M., Scholz,O., Lochner,S., Gmeiner,P. and Hillen,W. (2004) Structure-based design of Tet repressor to optimize a new inducer specificity. Biochemistry, 43, 9512-9518.
32. Backes,H., Berens,C., Helbl,V., Walter,S., Schmid,F.X. and Hillen,W. (1997) Combinations of the alpha-helix-turn-alpha-helix motif of TetR with respective residues from LacI or 434Cro: DNA recognition, inducer binding, and urea-dependent denaturation. Biochemistry, 36, 5311-5322.
33. Schubert,P., Schnappinger,D., Pfleiderer,K. and Hillen,W. (2001) Identification of a stability determinant on the edge of the tet repressor four-helix bundle dimerization motif. Biochemistry, 40, 3257-3263.
34. Simoncsits,A., Chen,J., Percipalle,P., Wang,S., Toro,I. and Pongor,S. (1997) Single-chain repressors containing engineered DNA-binding domains of the phage 434 repressor recognize symmetric or asymmetric DNA operators. J. Mol. Biol., 267, 118-131.
35. Robinson,C.R. and Sauer,R.T. (1998) Optimizing the stability of single-chain proteins by linker length and composition mutagenesis. Proc. Natl Acad. Sci. USA, 95, 5929-5934.
36. Jana,R., Hazbun,T.R., Fields,J.D. and Mossing,M.C. (1998) Single-chain lambda Cro repressors confirm high intrinsic dimer-DNA affinity. Biochemistry, 37, 6446-6455.
37. Flynn,T.C., Swint-Kruse,L., Kong,Y., Booth,C., Matthews,K.S. and Ma,J. (2003) Allosteric transition pathways in the lactose repressor protein core domains: Asymmetric motions in a homodimer. Protein Sci., 12, 2523-2541.