Large Conformational Changes in a Kinesin Motor Catalyzed by Interaction with Microtubules

Molecular Cell

Volumn 23, Issue 6, 2006, Pages 913-923

  Hirose, Keiko ^a   Akimaru, Erika ^a   Akiba, Toshihiko ^a   Endow, Sharyn A ^b   Amos, Linda A ^c  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b DUKE UNIVERSITY MEDICAL CENTER   (United States)

^c MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

CELLBIO

Indexed keywords

ADENOSINE DIPHOSPHATE; ADENOSINE PHOSPHATE; ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATE; KINESIN; MOLECULAR MOTOR; MYOSIN; NUCLEOTIDE; PROTEIN KAR3; PROTEIN KIF1A; TUBULIN; UNCLASSIFIED DRUG;

ALPHA HELIX; ARTICLE; BETA SHEET; CATALYSIS; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CRYOELECTRON MICROSCOPY; CRYSTAL STRUCTURE; MICROTUBULE; NONHUMAN; PROTEIN CONFORMATION; SWINE; THREE DIMENSIONAL IMAGING;

ADENOSINE DIPHOSPHATE; ADENYLYL IMIDODIPHOSPHATE; BINDING SITES; CRYOELECTRON MICROSCOPY; CRYSTALLOGRAPHY, X-RAY; KINESIN; MICROTUBULES; MODELS, MOLECULAR; PROTEIN STRUCTURE, TERTIARY; TUBULIN;

EID: 33748421625     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molcel.2006.07.020     Document Type: Article

References (38)

1. Al-Bassam J., Cui Y., Klopfenstein D., Carragher B.O., Vale R.D., and Milligan R.A. Distinct conformations of the kinesin Unc104 neck regulate a monomer to dimer motor transition. J. Cell Biol. 163 (2003) 743-753
2. Arnal I., and Wade R.H. Nucleotide-dependent conformations of the kinesin dimer interacting with microtubules. Structure 6 (1998) 33-38
3. Coureux P.D., Wells A.L., Menetrey J., Yengo C.M., Morris C.A., Sweeney H.L., and Houdusse A. A structural state of the myosin V motor without bound nucleotide. Nature 425 (2003) 419-423
4. Endow S.A., Kang S.J., Satterwhite L.L., Rose M.D., Skeen V.P., and Salmon E.D. Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends. EMBO J. 13 (1994) 2708-2713
5. Endres N.F., Yoshioka C., Milligan R.A., and Vale R.D. A lever-arm rotation drives motility of the minus-end-directed kinesin Ncd. Nature 439 (2006) 875-878
6. Frank J., Radermacher M., Penczek P., Zhu J., Li Y., Ladjadj M., and Leith A. SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related fields. J. Struct. Biol. 116 (1996) 190-199
7. Gulick A.M., Song H., Endow S.A., and Rayment I. X-ray crystal structure of the yeast Kar3 motor domain complexed with Mg.ADP to 2.3 Å resolution. Biochemistry 37 (1998) 1769-1776
8. Hirose K., Lockhart A., Cross R.A., and Amos L.A. Nucleotide-dependent angular change in kinesin motor domain bound to tubulin. Nature 376 (1995) 277-279
9. Hirose K., Cross R.A., and Amos L.A. Nucleotide-dependent structural changes in dimeric NCD molecules complexed to microtubules. J. Mol. Biol. 278 (1998) 389-400
10. Hirose K., Löwe J., Alonso M., Cross R.A., and Amos L.A. Congruent docking of dimeric kinesin and ncd into three-dimensional electron cryomicroscopy maps of microtubule-motor ADP complexes. Mol. Biol. Cell 10 (1999) 2063-2074
11. Hirose K., Henningsen U., Schliwa M., Toyoshima C., Shimizu T., Alonso M., Cross R.A., and Amos L.A. Structural comparison of dimeric Eg5, Neurospora kinesin (Nkin) and Ncd head-Nkin neck chimera with conventional kinesin. EMBO J. 19 (2000) 5308-5314
12. Hoenger A., Sack S., Thormahlen M., Marx A., Muller J., Gross H., and Mandelkow E. Image reconstructions of microtubules decorated with monomeric and dimeric kinesins: comparison with x-ray structure and implications for motility. J. Cell Biol. 141 (1998) 419-430
13. Hoenger A., Thormahlen M., Diaz-Avalos R., Doerhoefer M., Goldie K.N., Muller J., and Mandelkow E. A new look at the microtubule binding patterns of dimeric kinesins. J. Mol. Biol. 297 (2000) 1087-1103
14. Howard J. Mechanics of Motor Proteins and the Cytoskeleton (2001), Sinauer Associates, Inc., Sunderland, Massachusetts
15. Jones T.A., Zou J.Y., Cowan S.W., and Kjeldgaard. Improved methods for building protein models in electron density maps and the location of errors in these models. Acta. Crystallogr. A 47 Pt 2 (1991) 110-119
16. Kikkawa M., Okada Y., and Hirokawa N. 15 Å resolution model of the monomeric kinesin motor, Kif1A. Cell 100 (2000) 241-252
17. Kikkawa M., Sablin E.P., Okada Y., Yajima H., Fletterick R.J., and Hirokawa N. Switch-based mechanism of kinesin motors. Nature 411 (2001) 439-445
18. Kozielski F., De Bonis S., Burmeister W.P., Cohen-Addad C., and Wade R.H. The crystal structure of the minus-end-directed microtubule motor protein ncd reveals variable dimer conformations. Structure 7 (1999) 1407-1416
19. Kull F.J., and Endow S.A. Kinesin: switch I & II and the motor mechanism. J. Cell Sci. 115 (2002) 15-23
20. Kull F.J., and Endow S.A. A new structural state of myosin. Trends Biochem. Sci. 29 (2004) 103-106
21. Löwe J., Li H., Downing K.H., and Nogales E. Refined structure of alpha beta-tubulin at 3.5 Å resolution. J. Mol. Biol. 313 (2001) 1045-1057
22. Meluh P.B., and Rose M.D. KAR3, a kinesin-related gene required for yeast nuclear fusion. Cell 60 (1990) 1029-1041
23. Nitta R., Kikkawa M., Okada Y., and Hirokawa N. Kif1A alternately uses two loops to bind microtubules. Science 305 (2004) 678-683
24. Okada Y., and Hirokawa N. Mechanism of the single-headed processivity: diffusional anchoring between the K-loop of kinesin and the C terminus of tubulin. Proc. Natl. Acad. Sci. USA 97 (2000) 640-645
25. Ray S., Meyhofer E., Milligan R.A., and Howard J. Kinesin follows the microtubule's protofilament axis. J. Cell Biol. 121 (1993) 1083-1093
26. Reubold T.F., Eschenburg S., Becker A., Kull F.J., and Manstein D.J. A structural model for actin-induced nucleotide release in myosin. Nat. Struct. Biol. 10 (2003) 826-830
27. Sablin E.P., and Fletterick R.J. Nucleotide switches in molecular motors: structural analysis of kinesins and myosins. Curr. Opin. Struct. Biol. 11 (2001) 716-724
28. Sablin E.P., Case R.B., Dai S.C., Hart C.L., Ruby A., Vale R.D., and Fletterick R.J. Direction determination in the minus-end-directed kinesin motor ncd. Nature 395 (1998) 813-816
29. Sack S., Kull F.J., and Mandelkow E. Motor proteins of the kinesin family. Structures, variations, and nucleotide binding sites. Eur. J. Biochem. 262 (1999) 1-11
30. Shipley K., Hekmat-Nejad M., Turner J., Moores C., Anderson R., Milligan R., Sakowicz R., and Fletterick R. Structure of a kinesin microtubule depolymerization machine. EMBO J. 23 (2004) 1422-1432
31. Song H., and Endow S.A. Rapid purification of microtubule motor domain proteins expressed in bacteria. Biotechniques 22 (1997) 82-85
32. Song H., and Endow S.A. Decoupling of nucleotide- and microtubule-binding sites in a kinesin mutant. Nature 396 (1998) 587-590
33. Tani K., Sasabe H., and Toyoshima C. A set of computer programs for determining defocus and astigmatism in electron images. Ultramicroscopy 65 (1996) 31-44
34. Toyoshima C. Structure determination of tubular crystals of membrane proteins. I. Indexing of diffraction patterns. Ultramicroscopy 84 (2000) 1-14
35. Vale R.D., and Milligan R.A. The way things move: looking under the hood of molecular motor proteins. Science 288 (2000) 88-95
36. Wendt T.G., Volkmann N., Skiniotis G., Goldie K.N., Muller J., Mandelkow E., and Hoenger A. Microscopic evidence for a minus-end-directed power stroke in the kinesin motor ncd. EMBO J. 21 (2002) 5969-5978
37. Yun M., Zhang X., Park C.G., Park H.W., and Endow S.A. A structural pathway for activation of the kinesin motor ATPase. EMBO J. 20 (2001) 2611-2618
38. Yun M., Bronner C.E., Park C.G., Cha S.S., Park H.W., and Endow S.A. Rotation of the stalk/neck and one head in a new crystal structure of the kinesin motor protein, Ncd. EMBO J. 22 (2003) 5382-5389