Random insertion of a TetR-inducing peptide tag into Escherichia coli proteins allows analysis of protein levels by induction of reporter gene expression

Applied and Environmental Microbiology

Volumn 72, Issue 8, 2006, Pages 5637-5642

  Schlicht, Maximilian ^a   Berens, Christian ^a   Daam, Janko ^a   Hillen, Wolfgang ^a  

^a UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI; GENES; GENETIC ENGINEERING; PROTEINS;

GENE EXPRESSION; MICROARRAYS; PEPTIDE TAG; TRANSCRIPTIONAL FUSIONS;

BACTERIOLOGY;

BACTERIAL PROTEIN; BETA GALACTOSIDASE; HYBRID PROTEIN; MUTANT PROTEIN; POLYPEPTIDE; REPRESSOR PROTEIN; TRANSCRIPTION INDUCING PEPTIDE; UNCLASSIFIED DRUG;

COLIFORM BACTERIUM; GENE EXPRESSION; PEPTIDE; PROTEIN;

ARTICLE; CELLULAR DISTRIBUTION; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; GENE INSERTION SEQUENCE; INSERTION ELEMENT INSTIPALPHA; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEOMICS; REPORTER GENE; REPRESSOR GENE;

DNA TRANSPOSABLE ELEMENTS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENES, REPORTER; MUTATION; PEPTIDES; RECOMBINANT FUSION PROTEINS; TETRACYCLINE RESISTANCE; TRANSCRIPTION, GENETIC;

ESCHERICHIA COLI;

EID: 33747335912     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.00739-06     Document Type: Article

References (35)

1. Baranova, N., and H. Nikaido. 2002. The BaeSR two-component regulatory system activates transcription of the yegMNOB (mdtABCD) transporter gene cluster in Escherichia coli and increases its resistance to novobiocin and deoxycholate. J. Bacteriol. 184:4168-4176.
2. Bertram, R., M. Köstner, J. Müller, J. Vazquez Ramos, and W. Hillen. 2005. Integrative elements for Bacillus subtilis yielding tetracycline-dependent growth phenotypes. Nucleic Acids Res. 33:el53.
3. Brent, R., and M. Ptashne. 1981. Mechanism of action of the lexA gene product. Proc. Natl. Acad. Sci. USA 78:4204-4208.
4. R cassettes with the option of Flp-analyzed excision of the antibiotic-resistance determinant. Gene 158:9-14.
5. Dartigalongue, C., and S. Raina. 1998. A new heat-shock gene, ppiD, encodes a peptidyl-prolyl isomerase required for folding of outer membrane proteins in Escherichia coli. EMBO J. 17:3968-3980.
6. Gerdes, S. Y., M. D. Scholle, J. W. Campbell, G. Balázsi, E. Ravas, M. D. Daugherty, A. L. Somera, N. C. Kyrpides, I. Anderson, M. S. Gelland, A. Bhattacharya, V. Kapatral, M. D'Souza, M. V. Baev, Y. Grechkin, K. Mseeh, M. Y. Fonstein, R. Overbeek, A. L. Barabási, Z. N. Oltvai, and A. L. Osterman. 2003. Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J. Bacteriol. 185:5673-5684.
7. Ghaemmaghami, S., W. K. Huh, K. Bower, R. W. Howson, A. Belle, N. Dephoure, E. K. O'Shea, and J. S. Weissman. 2003. Global analysis of protein expression in yeast. Nature 425:737-741.
8. Goryshin, I. Y., J. Jendrisak, L. M. Hoffman, R. Meis, and W. S. Reznikoff. 2000. Insertional transposon mutagenesis by electroporation of released Tn5 transposition complexes. Nat. Biotechnol. 18:97-100.
9. Griffin, T. J., D. R. Goodlett, and R. Aebersold. 2001. Advances in proteome analysis by mass spectrometry. Curr. Opin. Biotechnol. 12:607-612.
10. Guérout-Fleury, A.-M., K. Shazand, N. Frandsen, and P. Stragier. 1995. Antibiotic-resistance cassettes for Bacillus subtilis. Gene 167:335-336.
11. Haddadin, F. T., and S. W. Harcum. 2005. Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli. Biotechnol. Bioeng. 90:127-153.
12. Hoffman, L. M., J. J. Jendrisak, R. J. Meis, I. Y. Goryshin, and W. S. Reznlkoff. 2000. Transposome insertional mutagenesis and direct sequencing of microbial genomes. Genetica 108:19-24.
13. Huh, W. K., J. V. Falvo, L. C. Gerke, A. S. Carroll, R. W. Howson, J. S. Weissman, and E. K. O'Shea. 2003. Global analysis of protein localization in budding yeast. Nature 425:686-691.
14. o ATPase subunit c and cytoplasmic pH regulation. J. Bacteriol. 183:1524-1530.
15. Kang, Y., T. Durfee, J. D. Glasner, Y. Qiu, D. Frisch, K. M. Winterherg, and F. R. Bluttner. 2004. Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186:4921-4930.
16. Klotzsche, M., C. Berens, and W. Hillen. 2005. A peptide triggers allostery in Tet repressor by binding to a unique site. J. Biol. Chem. 280:24591-24599.
17. Köstner, M., B. Schmidt, R. Bertram, and W. Hillen. 2006. Generating tetracyelinc-inducible auxotrophy in Escherichia coli and Salmonella enterica serovar Typhimurium by using an insertion clement and a hyperactive transposase. Appl. Environ. Microbiol. 72:4717-4725.
18. Lee, R. S., J. Pagan, S. Wilke-Mounts, and A. E. Senior. 1991. Characterization of Escherichia coli ATP synlhase β-subunit mutations using a chromosomal deletion strain. Biochemistry 30:6842-6847.
19. Lesley, S. A., J. Graziano, C. Y. Cho, M. W. Knuth, and H. E. Klock. 2002. Gene expression response to misfolded protein as a screen for soluble recombinant protein. Protein Eng. 15:153-160.
20. 2 regulatory elements. Nucleic Acids Res. 25:1203-1210.
21. Minoil, C., J. J. Mekalanos, and J. Beckwith. 1990. Alkaline phosphatase fusions: sensors of subcellular location. J. Bacteriol. 172:515-518.
22. Masuda, N., and G. M. Church. 2002. Escherichia coli gene expression responsive to levels of the response regulator EvgA. J. Bacteriol. 184:6225-6234.
23. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
24. Nishino, K., T. Honda, and A. Yamaguchi. 2005. Genome-wide analyses of Escherichia coli gene expression responsive to the BaeSR two-component regulatory system. J. Bacteriol. 187:1763-1772.
25. Rabilloud, T. 2002. Two-dimensional gel electrophoresis in proteomics: old, old fashioned, but it still climbs up the mountains. Proteomics 2:3-10.
26. Reznikoff, W. S. 2003. Tn5 as a model for understanding DNA transposition. Mol. Microbiol. 47:1199-1206.
27. Riley, M., T. Abe, M. B. Arnaud, M. K. Berlyn, F. R. Blattner, R. R. Chaudhuri, J. D. Glasner, T. Horiuchi, I. M. Keseler, T. Kosuge, H. Mori, N. T. Perna, G. Plunkett III, K. E. Rudd, M. H. Serres, G. H. Thomas, N. R. Thomson, D. Wishart, and B. L. Wanner. 2006. Escherichia coli K-12: a cooperatively developed annotation snapshot-2005. Nucleic Acids Res. 34: 1-9.
28. Ross-Macdonald, P., P. S. Coelho, T. Roemer, S. Agarwal, A. Kumar, R. Jansen, K. H. Cheung, A. Sheehan, D. Symoniatis, L. Umansky, M. Heidtman, F. K. Nelson, H. Iwasaki, K. Hager, M. Gerstein, P. Miller, G. S. Reeder, and M. Snyder. 1999. Large-scale analysis of the yeast genome by transposon tagging and gene disruption. Nature 402:413-418.
29. 0-ATPase. J. Biol. Chem. 271:33390-33393.
30. Schollmeier, K., D. Gärtner, and W. Hillen. 1985. A bidirectionally active signal for termination of transcription is located between tetA and orfL on transposon Tn10. Nucleic Acids Res. 13:4227-4237.
31. Selinger, D. W., K. J. Cheung, R. Mei, E. M. Johansson, C. S. Richmond, F. R. Blattner, D. J. Lockhart, and G. M. Church. 2000. RNA expression analysis using a 30 base pair resolution Escherichia coli genome array. Nat. Biotechnol. 18:1262-1268.
32. Smith, L. D., and K. P. Bertrand. 1988. Mutations in the Tn10 let repressor that interfere with induction. Location of the tetracycline-binding domain. J. Mol. Biol. 203:949-959.
33. Uzzau, S., N. Figueroa-Bossi, S. Rubino, and L. Bossi. 2001. Epitope tagging of chromosomal genes in Salmonella. Proc. Natl. Acad. Sci. USA 98:15264-15269.
34. Wise, J. G. 1990. Site-directed mutagenesis of the conserved β subunit tyrosine 331 of Escherichia coli ATP synthase yields catalytically active enzymes. J. Biol. Chem. 265:10403-10409.
35. Wissmann, A., L. V. Wray, Jr., U. Somaggio, R. Baumeister, M. Geissendörfer, and W. Hillen. 1991. Selection for Tn10 Tet repressor binding to tet operator in Escherichia coli: isolation of temperature-sensitive mutants and combinatorial mutagenesis in the DNA binding motif. Genetics 128:225-232.