Generating tetracycline-inducible auxotrophy in Escherichia coli and Salmonella enterica serovar typhimurium by using an insertion element and a hyperactive transposase

Applied and Environmental Microbiology

Volumn 72, Issue 7, 2006, Pages 4717-4725

  Köstner, Martin ^a   Schmidt, Britta ^a   Bertram, Ralph ^a   Hillen, Wolfgang ^a  

^a UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; CELLS; ENZYMES; GENES; GENETIC ENGINEERING; GROWTH KINETICS; MICROBIOLOGY; MUTAGENESIS;

AUXOTROPHIC MUTANTS; HYPERACTIVE TRANSPOSASE; INSERTION ELEMENT; SALMONELLA ENTERICA SEROVAR TYPHIMURIUM;

ESCHERICHIA COLI;

AMPICILLIN; ANHYDROTETRACYCLINE; KANAMYCIN; TETRACYCLINE; TRANSPOSASE;

BACTERIUM; MUTATION;

ANIMAL CELL; ARGA GENE; ARTICLE; AUXOTROPHY; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM MUTANT; CELL GROWTH; CELL LINE; COMPLEX FORMATION; CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; GENE INSERTION SEQUENCE; GROWTH RATE; KNOCKOUT GENE; MOUSE; MUTAGENESIS; NONHUMAN; PROMOTER REGION; PYRE GENE; REPRESSOR GENE; REVERTANT; SALMONELLA ENTERICA; SEROTYPE;

ANIMALS; BASE SEQUENCE; CELL LINE; DNA TRANSPOSABLE ELEMENTS; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; MACROPHAGES; MICE; MOLECULAR SEQUENCE DATA; MUTATION; PROMOTER REGIONS (GENETICS); SALMONELLA TYPHIMURIUM; TETRACYCLINES; TRANSPOSASES;

ESCHERICHIA COLI; NEGIBACTERIA; SALMONELLA; SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHIMURIUM;

EID: 33746032574     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.00492-06     Document Type: Article

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