Transposome insertional mutagenesis and direct sequencing of microbial genomes

Genetica

Volumn 108, Issue 1, 2000, Pages 19-24

  Hoffman, Les M ^a   Jendrisak, Jerry J ^a   Meis, Ronald J ^a   Goryshin, Igor Y ^b   Reznikoff, William S ^b  

^a ILLUMINA INC   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Genomic sequencing; Mutagenesis; Transposome

Indexed keywords

ANTIBIOTIC RESISTANCE; BACTERIAL CELL; BACTERIAL DNA; ELECTROPORATION; IN VIVO STUDY; INSERTIONAL MUTAGENESIS; KANAMYCIN; PHENOTYPE; TRANSPOSASE; TRANSPOSON;

CHROMOSOMES, BACTERIAL; DNA TRANSPOSABLE ELEMENTS; DNA, BACTERIAL; ELECTROPORATION; ENTEROBACTERIACEAE; GENOME, BACTERIAL; MAGNESIUM; MUTAGENESIS, INSERTIONAL; PSEUDOMONAS; SEQUENCE ANALYSIS, DNA; TRANSPOSASES;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; PROKARYOTA;

EID: 0034534135     PISSN: 00166707     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1004083307819     Document Type: Article

References (12)

1. Identification of a gene, PilV required for type 4 fimbfial biogenesis in Pseudornonas aeruginosa, whose product possesses a pre-pilin-like leader sequence
2. Differentiation of bacterial 16S rRNA genes and intergenic regions and Mycobacteruim tuberculosis katG genes by structure-specific endonuclease cleavage
3. Tn5 in vitro transposition
4. Insertional transposon mutagenesis by electroporation of released Tn5 transposition complexes
5. Sequencing mutimegabase-template DNA with BigDye terminator chemistry
6. In vivo transposition of transposon/ transposase complexes into the genorne of Saccharomyces
7. Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K
8. Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6K gamma origin plasmids at different copy numbers
9. Phylogenetic and physiological comparisons of PAH-degrading bacteria from geographically diverse soils
10. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae
11. Simple and efficient generation in vitro of nested deletions and inversions: Tn5 intrarnolecular transposition
12. Molecular genetic analysis of transposase-end sequence recognition: Cooperation of three adjacent base pairs in specific interaction with a mutant Tn5 transposase