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Dedieu, J.-C.4
Vautrin, C.5
Désert, S.6
Perez, E.7
Zemb, T.8
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74
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33747197518
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note
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Longer reconstitution times resulted in a decrease in activity. When the reconstitution was performed at 4°C no enzymatic activity was observed. Also the use of other cofactor solvents such as DMF did not result in an increase of enzymatic activity, rather a precipitate indicative of denaturation was observed after 4 days.
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75
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33747191794
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-
note
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When le was incubated with glucose oxidase (GOx) and acylase, two non-heme enzymes containing several lysine and histidine residues on their periphery, no peroxidase activity was observed. This implies that the enzymatic activity of le incubated with apo-HRP is not caused by the interaction of le with amino acid residues on the apo-HRP periphery.
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76
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0028890688
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M. Arnold, P. Ringler, A. Brisson, Biochim, Biophys. Acta 1995, 1233, 198.
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Arnold, M.1
Ringler, P.2
Brisson, A.3
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77
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33747190933
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note
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The scattering may be also partially caused by the presence of some denaturated protein.
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78
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0040487502
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T. Hayashi, Y. Hitomi, A. Suzuki, T. Takamura, H. Ogoshi, Chem. Lett. 1995, 911.
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84891514224
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Carboxy-terminated polystyrene was synthesized according to a literature procedure: J. C. M. v. Hest, D. A. P. Delnoye, M. W. P. L. Baars, C. Elissen-Román, M. H. P. v. Genderen, E. W. Meijer, Chem. Eur. J. 1996, 2, 1616-1626.
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Genderen, M.H.P.V.5
Meijer, E.W.6
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