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0344445909
-
-
note
-
In contrast, for the intermolecular reaction, negative peaks at 208 and 222 nm and a positive peak at 195 nm due to a typical α-helix in the CD spectrum are gradually lessened and lost during photolysis. The results obtained from HPLC showed that, during the photolysis, the peak intensity due to protoheme and the protein skeleton is reduced for the intermolecular system. In SDS - PAGE, the original band due to apo-Mb is smeared, and several bands are observed. These suggest that the Mb structure was considerably damaged in the intermolecular photooxidation process.
-
-
-
-
47
-
-
0344014615
-
-
note
-
A sharp Soret-band is never regenerated by addition of 2-methoxyphenol.
-
-
-
-
48
-
-
0344014614
-
-
note
-
8c
-
-
-
-
49
-
-
0000793939
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Gans, P.; Buisson, G.; Duée, E.; Marchon, J.-C.; Erler, B. S.; Scholz, W. F.; Reed, C. A. J. Am. Chem. Soc. 1986, 108, 1223.
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Reed, C.A.7
-
50
-
-
0344014613
-
-
note
-
1 at pH 9.0 independently.
-
-
-
-
51
-
-
0344445906
-
-
note
-
1.
-
-
-
-
52
-
-
0344445907
-
-
note
-
3.
-
-
-
-
53
-
-
0344445905
-
-
note
-
10 (25) In eqs 3 and 7, it is not clear so far that electron and proton transfers are concerted or stepwise. It is only insisted that this process includes one kinetic rate needed for fitting the present pH dependence.
-
-
-
-
54
-
-
0344877681
-
-
note
-
2O, we cannot conduct the curve-fitting analysis.
-
-
-
-
55
-
-
0004155427
-
-
Freeman: New York
-
a of ε-N of Lys in general is about 10 ± 1. See: Biochemistry, 3rd ed.; Stryer, L., Ed.; Freeman: New York, 1988.
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Biochemistry, 3rd Ed.
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Stryer, L.1
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Carver, P. E.; Olson, J. S.; Smerdon, S. J.; Krzywda, S.; Wilkinson, A. J.; Gibson, Q. H.; Blackmore, R. S.; Ropp, J. D.; Sligar, S. G. Biochemistry 1991, 30, 4697.
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Sligar, S.G.9
-
58
-
-
0344877680
-
-
note
-
3-Mb, more physicochemical experiments using various mutant Mbs are required.
-
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-
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59
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