Thin layer chromatography-blotting, a novel method for the detection of phosphoinositides

Journal of Biochemistry

Volumn 139, Issue 4, 2006, Pages 663-670

  Furutani, Masahiro ^a   Itoh, Toshiki ^a   Ijuin, Takeshi ^a   Tsujita, Kazuya ^a   Takenawa, Tadaomi ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

Cancer; Insulin; Phosphoinositide; Phosphoinositide binding domain; Thin layer chromatography (TLC) blotting

Indexed keywords

INSULIN; PHOSPHATIDYLINOSITIDE; PHOSPHATIDYLINOSITIDE 3 P2; PHOSPHATIDYLINOSITIDE 3 P3; PHOSPHATIDYLINOSITIDE 4 P2; PHOSPHATIDYLINOSITIDE 4 P3; PHOSPHATIDYLINOSITIDE 5 P3; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BLOTTING; CANCER; CANCER CELL CULTURE; CONTROLLED STUDY; DIFFERENTIAL DIAGNOSIS; HUMAN; HUMAN CELL; IN VIVO STUDY; METABOLIC DISORDER; MOUSE; NONHUMAN; PHYSIOLOGY; PROTEIN ANALYSIS; PROTEIN DOMAIN; THIN LAYER CHROMATOGRAPHY;

1-PHOSPHATIDYLINOSITOL 4-KINASE; ANIMALS; CELLS, CULTURED; CHO CELLS; CHROMATOGRAPHY, THIN LAYER; CRICETINAE; HUMANS; IMMUNOBLOTTING; INSULIN; LIPID METABOLISM; MELANOMA, EXPERIMENTAL; MICE; PHOSPHATIDYLINOSITOL PHOSPHATES; PROTO-ONCOGENE PROTEINS C-AKT; PTEN PHOSPHOHYDROLASE; TUMOR CELLS, CULTURED;

EID: 33745667306     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvj076     Document Type: Article

References (26)

1. Takenawa, T. and Itoh, T. (2001) Phosphoinositides, key molecules for regulation of actin cytoskeletal organization and membrane traffic from the plasma membrane. Biochim. Biophys. Acta 1533, 190-206
2. Rohatgi, R., Ho, H.Y., and Kirschner, M.W. (2000) Mechanism of N-WASP activation by CDC42 and phosphatidylinositol 4, 5-bisphosphate. J. Cell. Biol. 150, 1299-1310
3. Roth, M.G. (2004) Phosphoinositides in constitutive membrane traffic. Physiol. Rev. 84, 699-730
4. Pendaries, C., Tronchere, H., Plantavid, M., and Payrastre, B. (2003) Phosphoinositide signaling disorders in human diseases. FEBS. Lett. 546, 25-31
5. Dressman, M.A., Olivos-Glander, I.M., Nussbaum, R.L., and Suchy, S.F (2000) Ocrl1, a PtdIns(4,5)P(2) 5-phosphatase, is localized to the trans-Golgi network of fibroblasts and epithelial cells. J. Histochem. Cytochem. 48, 179-190
6. Nystuen, A., Legare, M.E., Shultz, L.D., and Frankel, W.N. (2001) A null mutation in inositol polyphosphate 4-phosphatase type I causes selective neuronal loss in weeble mutant mice. Neuron 32, 203-212
7. Serunian, L.A., Auger, K.R., and Cantley, L.C. (1991) Identification and quantification of polyphosphoinositides produced in response to platelet-derived growth factor stimulation. Methods Enzymol. 198, 78-87
8. Wenk, M.R., Lucast, L., Di Paolo, G., Romanelli, A.J., Suchy, S.F., Nussbaum, R.L., Cline, G.W., Shulman, G.I., McMurray, W., and De Camilli, P. (2003) Phosphoinositide profiling in complex lipid mixtures using electrospray ionization mass spectrometry. Nat. Biotechnol. 21, 813-817
9. Klarlund, J.K., Tsiaras, W., Holik, J.J., Chawla, A., and Czech, M.P. (2000) Distinct polyphosphoinositide binding selectivities for pleckstrin homology domains of GRP1-like proteins based on diglycine versus triglycine motifs. J. Biol. Chem. 275, 32816-32821
10. Gray, A., Van Der Kaay, J., and Downes, C.P. (1999) The pleckstrin homology domains of protein kinase B and GRP1 (general receptor for phosphoinositides-1) are sensitive and selective probes for the cellular detection of phosphatidylinositol 3,4-bisphosphate and/or phosphatidylinositol 3,4,5-trisphosphate in vivo. Biochem. J. 344 Pt 3, 929-936
11. Dowler, S., Currie, R.A., Campbell, D.G., Deak, M., Kular, G., Downes, C.P., and Alessi, D.R. (2000) Identification of pleckstrin-homology-domain- containing proteins with novel phosphoinositide-binding specificities. Biochem. J. 351, 19-31
12. Gillooly, D.J., Morrow, I.C., Lindsay, M., Gould, R., Bryant, N.J., Gaullier, J.M., Parton, R.G., and Stenmark, H. (2000) Localization of phosphatidylinositol 3-phosphate in yeast and mammalian cells. EMBO J. 19, 4577-4588
13. Levine, T.P. and Munro, S. (2002) Targeting of Golgi-specific pleckstrin homology domains involves both PtdIns 4-kinase-dependent and -independent components. Curr. Biol. 12, 695-704
14. Itoh, T. and Takenawa, T. (2002) Phosphoinositide-binding domains: Functional units for temporal and spatial regulation of intracellular signalling. Cell. Signal. 14, 733-743
15. Lemmon, M.A. (2003) Phosphoinositide recognition domains. Traffic 4, 201-213
16. Stillway, L.W. and Harmon, S.J. (1980) A procedure for detecting phosphonolipids on thin-layer chromatograms. J. Lipid. Res. 21, 1141-1143
17. Ishikawa, D. and Taki, T. (2000) Thin-layer chromatography blotting using polyvinylidene difluoride membrane (far-eastern blotting) and its applications. Methods. Enzymol. 312, 145-157
18. Ruderman, N.B., Kapeller, R., White, M.F., and Cantley, L.C. (1990) Activation of phosphatidylinositol 3-kinase by insulin. Proc. Natl. Acad. Sci. USA 87, 1411-1415
19. Wymann, M.P., Bulgarelli-Leva, G., Zvelebil, M.J., Pirola, L., Vanhaesebroeck, B., Waterfield, M.D., and Panayotou, G. (1996) Wortmannin inactivates phosphoinositide 3-kinase by covalent modification of Lys-802, a residue involved in the phosphate transfer reaction. Mol. Cell. Biol. 16, 1722-1733
20. Walker, E.H., Pacold, M.E., Perisic, O., Stephens, L., Hawkins, P.T., Wymann, M.P., and Williams, R.L. (2000) Structural determinants of phosphoinositide 3-kinase inhibition by wortmannin, LY294002, quercetin, myricetin, and staurosporine. Mol. Cell. 6, 909-919
21. Maehama, T. and Dixon, J.E. (1998) The tumor suppressor, PTEN/MMAC1, dephosphorylates the lipid second messenger, phosphatidylinositol 3,4,5-trisphosphate. J. Biol. Chem. 273, 13375-13378
22. Myers, M.P., Pass, I., Batty, I.H., Van der Kaay, J., Stolarov, J.P., Hemmings, B.A., Wigler, M.H., Downes, C.P., and Tonks, N.K. (1998) The lipid phosphatase activity of PTEN is critical for its tumor supressor function. Proc. Natl. Acad. Sci. USA 95, 13513-13518
23. Stambolic, V., Suzuki, A., de la Pompa, J.L., Brothers, G.M., Mirtsos, C., Sasaki, T., Ruland, J., Penninger, J.M., Siderovski, D.P., and Mak, T.W. (1998) Negative regulation of PKB/Akt-dependent cell survival by the tumor suppressor PTEN. Cell 95, 29-39
24. Adachi, J., Ohbayashi, K., Suzuki, T., and Sasaki, T. (1999) Cell cycle arrest and astrocytic differentiation resulting from PTEN expression in glioma cells. J. Neurosurg. 91, 822-830
25. Fidler, I.J. (1973) Selection of successive tumour lines for metastasis. Nat. New. Biol. 242, 148-149
26. Haas-Kogan, D., Shalev, N., Wong, M., Mills, G., Yount, G., and Stokoe, D. (1998) Protein kinase B (PKB/Akt) activity is elevated in glioblastoma cells due to mutation of the tumor suppressor PTEN/MMAC. Curr. Biol. 8, 1195-1198