Mechanism of N-WASP activation by CDC42 and phosphatidylinositol 4,5-bisphosphate

Journal of Cell Biology

Volumn 150, Issue 6, 2000, Pages 1299-1309

  Rohatgi, Rajat ^a   Ho, Hsin Yi Henry ^a   Kirschner, Marc W ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Actin; Cdc42 GTP binding protein; Phosphoinositides; Signal transduction; Wiskott Aldrich Syndrome protein

Indexed keywords

ARP 2 3 COMPLEX; CYCLIN DEPENDENT KINASE; MUTANT PROTEIN; PHOSPHATIDYLINOSITOL 4,5 BISPHOSPHATE; PROTEIN CDC42; RHO FACTOR; UNCLASSIFIED DRUG; WISKOTT ALDRICH SYNDROME PROTEIN;

ACTIN FILAMENT; ACTIN POLYMERIZATION; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SIGNAL TRANSDUCTION; WISKOTT ALDRICH SYNDROME; XENOPUS;

ACTIN-RELATED PROTEIN 2; ACTIN-RELATED PROTEIN 3; ACTINS; ANIMALS; BINDING SITES; CDC42 GTP-BINDING PROTEIN; CYTOSKELETAL PROTEINS; GTP-BINDING PROTEINS; NERVE TISSUE PROTEINS; OOCYTES; PHOSPHATIDYLINOSITOL 4,5-DIPHOSPHATE; POLYMERS; PROTEIN STRUCTURE, TERTIARY; RATS; SIGNAL TRANSDUCTION; WISKOTT-ALDRICH SYNDROME; WISKOTT-ALDRICH SYNDROME PROTEIN, NEURONAL; XENOPUS;

VESPIDAE;

EID: 0034683746     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.150.6.1299     Document Type: Article

References (36)

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2. Two GTPases, Cdc42 and Rac, bind directly to a protein implicated in the immunodeficiency disorder Wiskott-Aldrich Syndrome
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4. SCAR, a WASP-related protein, isolated as a suppressor of receptor defects in late Dictyostelium development
5. Actin polymerization: Where the WASP stings
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7. Pyrene actin: Documentation of the validity of a sensitive assay for actin polymerization
8. Isolation of a novel gene mutated in Wiskott-Aldrich syndrome
9. Activation of the CDC42 effector N-WASP by the Shigella flexneri IcsA protein promotes actin nucleation by Arp2/3 complex and bacterial actin-based motility
10. Rho GTPases and the actin cytoskeleton
11. Autoinhibition and activation mechanisms of the Wiskott-Aldrich syndrome protein
12. Direct interaction of the Wiskott-Aldrich syndrome protein with the GTPase Cdc42
13. Structure of PAK1 in an autoinhibited conformation reveals a multi-stage activation switch
14. Beel, a yeast protein with homology to Wiskott-Aldrich Syndrome protein, is critical for the assembly of cortical actin cytoskeleton
15. Corequirement of specific phosphoinositides and small GTP-binding protein Cdc42 in inducing actin assembly in Xenopus egg extracts
16. The Arp2/3 complex mediates actin polymerization induced by the small GTP-binding protein Cdc42
17. Signaling to actin dynamics
18. Scar, a WASp-related protein, activates nucleation of actin filaments by the Arp2/3 complex
19. Direct binding of the verprolin-homology domain in N-WASP to actin is essential for cytoskeletal reorganization
20. 2-dependent manner downstream of tyrosine kinases
21. Induction of filopodium formation by a WASP-related actin-depolymerizing protein N-WASP
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23. How WASP-family proteins and the Arp2/3 complex convert intracellular signals into cytoskeletal structures
24. The interaction of Arp2/3 complex with actin: Nucleation, high affinity pointed end capping, and formation of branching networks of filaments
25. A comparative analysis of the phosphoinositide binding specificity of pleckstrin homology domains
26. WIP, a protein associated with Wiskott-Aldrich Syndrome protein, induces actin polymerization and redistribution in lymphoid cells
27. Wiskott-Aldrich syndrome protein physically associates with Nck through Src homology 3 domains
28. The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly
29. Phosphatidylinositol 4,5-bisphosphate induces actin-based movement of raft-enriched vesicles through WASP-Arp2/3
30. Wiskott-Aldrich syndrome protein is associated with the adapter protein Grb2 and the epidermal growth factor receptor in living cells
31. Identification of two human WAVE/SCAR homologues as general actin regulatory molecules which associate with the Arp2/3 complex
32. Neural Wiskott-Aldrich syndrome protein is implicated in the actin-based motility of Shigella flexneri
33. Arp2/3 complex and actin depolymerizing factor/cofilin in dendritic organization and treadmilling of actin filament array in lamellipodia
34. Wiskott-Aldrich syndrome protein, a novel effector for the GTPase CDC42Hs, is implicated in actin polymerization
35. Activation of the yeast Arp2/3 complex by Bee1p, a WASP-family protein
36. The Wiskott-Aldrich syndrome protein directs actin-based motility by stimulating actin nucleation with the Arp2/3 complex