Translational initiation in Leishmania tarentolae and Phytomonas serpens (Kinetoplastida) is strongly influenced by pre-ATG triplet and its 5′ sequence context

Molecular and Biochemical Parasitology

Volumn 148, Issue 2, 2006, Pages 125-132

  Lukeš, Julius ^a   Paris, Zdeněk ^a   Regmi, Sandesh ^a   Breitling, Reinhard ^b   Mureev, Sergey ^c   Kushnir, Susanna ^c   Pyatkov, Konstantin ^d   Jirků, Milan ^a   Alexandrov, Kirill A ^c  

^a INSTITUTE OF PARASITOLOGY   (Czech Republic)

^b Jena Bioscience GmbH   (Germany)

^c MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

^d CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

Initiation; Kinetoplastida; Leishmania; Pre ATG; Translation

Indexed keywords

GREEN FLUORESCENT PROTEIN; PLASMID VECTOR; RED FLUORESCENT PROTEIN; RIBOSOME DNA;

ARTICLE; CODON; CONTROLLED STUDY; CORRELATION ANALYSIS; EXPRESSION VECTOR; GENE LOCUS; GENETIC CODE; LEISHMANIA TARENTOLAE; MOLECULAR EVOLUTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYTOMONAS; PHYTOMONAS SERPENS; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; RANDOMIZATION; REPORTER GENE; TRANSLATION INITIATION; WESTERN BLOTTING;

5' UNTRANSLATED REGIONS; ANIMALS; CODON, INITIATOR; GENE EXPRESSION REGULATION; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; LEISHMANIA; LUMINESCENT PROTEINS; PROTEIN BIOSYNTHESIS; TETRACYCLINE RESISTANCE; TRANSFECTION; TRYPANOSOMATINA;

KINETOPLASTIDA; LEISHMANIA TARENTOLAE; PHYTOMONAS SERPENS; TRYPANOSOMATIDAE;

EID: 33745351081     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2006.03.008     Document Type: Article

References (38)

1. Higgs D.C., Shapiro R.S., Kindle K.L., and Stern D.B. Small cis-acting sequences that specify secondary structures in a chloroplast mRNA are essential for RNA stability and translation. Mol Cell Biol 19 (1999) 8479-8491
2. Kozak M. Initiation of translation in prokaryotes and eukaryotes. Gene 234 (1999) 187-208
3. Vervoort E.B., van Ravenstein A., van Peij N.N.M.E., et al. Optimizing heterologous expression in Dictyostelium: importance of 5′ codon adaptation. Nucleic Acids Res 28 (2000) 2069-2074
4. Wilkie G.S., Dickson K.S., and Gray N.K. Regulation of mRNA translation by 5′-and 3′-UTR-binding factors. Trends Biochem Sci 28 (2003) 182-188
5. Kozak M. Regulation of translation via mRNA structure in prokaryotes and eukaryotes. Gene 361 (2005) 13-37
6. Kozak M. Recognition of AUG and alternative initiator codons is augmented by G in position +4 but is not generally affected by the nucleotides in positions +5 and +6. EMBO J 16 (1997) 2482-2492
7. Pestova T.V., and Kolupaeva V.G. The roles of individual eukaryotic translation initiation factors in ribosomal scanning and initiation codon selection. Genes Dev 16 (2002) 2906-2922
8. Kozak M. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44 (1986) 283-292
9. Yun D.F., Laz T.M., Clements J.M., and Sherman F. mRNA sequences influencing translation and the selection of AUG initiator codons in the yeast Saccharomyces cerevisiae. Mol Microbiol 19 (1996) 1225-1239
10. Esposito D., Hicks A.J., and Stern D.B. A role for initiation codon context in chloroplast translation. Plant Cell 13 (2001) 2373-2384
11. Pesole G., Gissi C., Grillo G., Licciulli F., Liuni S., and Saccone C. Analysis of oligonucleotide AUG start codon context in eukaryotic mRNAs. Gene 261 (2000) 85-91
12. Kozak M. Structural features in eukaryotic messenger RNAs that modulate the initiation of translation. J Biol Chem 266 (1991) 19867-19870
13. Clayton C.E. Life without transcriptional control? From fly to man and back again. EMBO J 21 (2002) 1881-1888
14. Johnson P.J., Kooter J.M., and Borst P. Inactivation of transcription by UV-irradiation of Trypanosoma brucei provides evidence for a multicistronic transcription unit including a VSG gene. Cell 51 (1987) 273-281
15. Martínez-Calvillo S., Nguyen D., Stuart K., and Myler P.J. Transcription initiation and termination on Leishmania major chromosome 3. Eukaryot Cell 3 (2004) 506-517
16. Myung K.S., Beetham J.K., Wilson M.E., and Donelson J.E. Comparison of the post-transcriptional regulation of the mRNAs for the surface proteins PSA (GP46) and MSP (GP63) of Leishmania chagasi. J Biol Chem 277 (2002) 16489-16497
17. D'Orso I., De Gaudenzi J.G., and Frasch A.C.C. RNA-binding proteins and mRNA turnover in trypanosomes. Trends Parasitol 19 (2003) 151-155
18. Requena J.M., Quijada L., Soto M., and Alonso C. Conserved nucleotides surrounding the trans-splicing acceptor site and the translation initiation codon in Leishmania genes. Exp Parasitol 103 (2003) 78-81
19. Yamauchi K. The sequence flanking translational initiation site in protozoa. Nucleic Acids Res 19 (1991) 2715-2720
20. Stanton D.J., and Mensa-Wilmot K. Control of protein expression by -3 to -1 nucleotides of Leishmania 5′ UTRs: identification of translational enhancers. Mol Biol Cell 11 (2000) 441A
21. Breitling R., Klinger S., Callewaert N., et al. Non-pathogenic trypanosomatid protozoa as a platform for protein research and production. Protein Expr Purif 25 (2002) 209-218
22. Kushnir S., Gase K., Breitling R., and Alexandrov K.A. Development of an inducible protein expression system based on the protozoan host Leishmania tarentolae. Protein Expr Purif 42 (2005) 37-46
23. Bevis B.J., and Glick B.S. Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed). Nat Biotechnol 20 (2002) 83-87
24. Robinson K.A., and Beverley S.M. Improvements in transfection efficiency and tests of RNA interference (RNAi) approaches in the protozoan parasite Leishmania. Mol Biochem Parasitol 128 (2003) 217-228
25. Pasion S.G., Hines J.C., Aebersold R., and Ray D.S. Molecular cloning and expression of the gene encoding the kinetoplast-associated type II DNA topoisomerase of Crithidia fasciculata. Mol Biochem Parasitol 50 (1992) 57-68
26. Wang B.B., Ernst N.L., Palazzo S.S., Panigrahi A.K., Salavati R., and Stuart K. TbMP44 is essential for RNA editing and structural integrity of the editosome in Trypanosoma brucei. Eukaryot Cell 2 (2003) 578-587
27. Yakhnin A.V., Vinokurov L.M., Surin A.K., and Alakhov Y.B. Green fluorescent protein purification by organic extraction. Protein Expr Purif 14 (1998) 382-386
28. Rutherford K., Parkhill J., Crook J., et al. Artemis: sequence visualization and annotation. Bioinformatics 16 (2000) 944-945
29. Biebinger S., Rettenmaier S., Flaspohler J., et al. The PARP promoter of Trypanosoma brucei is developmentally regulated in a chromosomal context. Nucleic Acids Res 24 (1996) 1202-1211
30. Jack H.M., Berg J., and Wabl M. Translation affects immunoglobulin mRNA stability. Eur J Immunol 19 (1989) 843-847
31. Braun F., Le Derout J., and Regnier P. Ribosomes inhibit an RNase E cleavage which induces the decay of the rpsO mRNA in Escherichia coli. EMBO J 17 (1998) 4790-4797
32. Fernandes A.P., Nelson K., and Beverley S.M. Evolution of nuclear ribosomal RNAs in kinetoplastid protozoa-perspectives on the age and origins of parasitism. Proc Natl Acad Sci USA 90 (1993) 11608-11612
33. Marché S., Roth C., Philippe H., Dollet M., and Baltz T. Characterization and detection of plant trypanosomatids by sequence analysis of the small subunit ribosomal RNA gene. Mol Biochem Parasitol 71 (1995) 15-26
34. Clayton C.E. Genetic manipulation of Kinetoplastida. Parasitol Today 15 (1999) 372-378
35. Berens C., and Hillen W. Gene regulation by tetracyclines. Eur J Biochem 270 (2003) 3109-3121
36. Baird G.S., Zacharias D.A., and Tsien R.Y. Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral. Proc Natl Acad Sci USA 97 (2000) 11984-11989
37. Webb H., Burns R., Ellis L., Kimblin N., and Carrington M. Developmentally regulated instability of the GPI-PLC mRNA is dependent on a short-lived protein factor. Nucleic Acids Res 33 (2005) 1503-1512
38. Ivens A.C., Peacock C.S., Worthey E.A., et al. The genome of the kinetoplastid parasite Leishmania major. Science 309 (2005) 436-442