Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed)

Nature Biotechnology

Volumn 20, Issue 1, 2002, Pages 83-87

  Bevis, B J ^a   Glick, B S ^a  

^a UNIVERSITY OF CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CHROMOPHORES; FLUORESCENCE; MUTAGENESIS; PROTEINS; SOLUBILITY; YEAST;

FLUORESCENT PROTEINS;

BIOTECHNOLOGY;

ASPARAGINE; GLUTAMINE; GREEN FLUORESCENT PROTEIN; PROTEIN; RED FLUORESCENT PROTEIN;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; CHROMATOPHORE; COLOR; CONTROLLED STUDY; CORAL; FLUORESCENCE; FUNGUS GROWTH; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROCESSING; PROTEIN VARIANT; SITE DIRECTED MUTAGENESIS; SOLUBILITY; SURFACE CHARGE; YEAST;

ANIMALS; ASPARAGINE; CNIDARIA; GENETIC TECHNIQUES; GLUTAMINE; KINETICS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; MUTAGENESIS; MUTAGENESIS, SITE-DIRECTED; PROTEIN STRUCTURE, TERTIARY; SACCHAROMYCES CEREVISIAE; TEMPERATURE; TIME FACTORS;

ANTHOZOA; DISCOSOMA; FUNGI;

EID: 0036138545     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0102-83     Document Type: Article

References (23)

1. Fluorescent proteins from nonbioluminescent Anthozoa species
2. Biochemistry, mutagenesis, and origomerization of DsRed, a red fluorescent protein from coral
3. EGFP and DsRed expressing cultures of Escherichia coli imaged by confocal, two-photon and fluorescence lifetime microscopy
4. Improved green fluorescence
5. FACS-optimized mutants of the green fluorescent protein (GFP)
6. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescent resonance energy transfer
7. 2+ based on green fluorescent proteins and calmodulin
8. The structural basis for red fluorescence in the tetrameric GFP homolog DsRed
9. Refined crystal structure of DsRed, a red fluorescent protein from coral, at 2.0-Å resolution
10. The structure of the chromophore within DsRed, a red fluorescent protein from coral
11. Four-color flow cytometric detection of retrovirally expressed red, yellow, green, and cyan fluorescent proteins
12. Living colors™ DsRed2
13. Expression of fluorescently tagged connexins: A novel approach to rescue function of oligomerrc DsRed-tagged proteins
14. Mutants of Discosoma red fluorescent protein with a GFP-like chromophore
15. "Fluorescent timer": Protein that changes color with time
16. A genetics-friendly GFP assay
17. Mutagenic PCR
18. An enhanced mutant of red fluorescent protein DsRed for double labeling and developmental timer of neural fiber bundle formation
19. A role for actin, Cdc1p and Myo2p in the inheritance of late Golgi elements in Saccharomyces cerevisiae
20. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae
21. Golgi structure correlates with transitional endoplasmic reticulum organization in Pichia pastoris and Saccharomyces cerevisiae