Mismatched siRNAs downregulate mRNAs as a function of target site location

FEBS Letters

Volumn 580, Issue 15, 2006, Pages 3694-3698

  Martin, Scott E ^a   Caplen, Natasha J ^a  

^a CCR   (United States)

Author keywords

3 UTR; miRNA; RNAi; siRNA

Indexed keywords

MESSENGER RNA; SMALL INTERFERING RNA;

3' UNTRANSLATED REGION; ARTICLE; CONTROLLED STUDY; DOWN REGULATION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; RNA INTERFERENCE; RNA TRANSCRIPTION;

ATP-BINDING CASSETTE TRANSPORTERS; BASE PAIR MISMATCH; BASE SEQUENCE; BETA CATENIN; BINDING SITES; CELL LINE, TUMOR; DOWN-REGULATION; HUMANS; RNA, MESSENGER; RNA, SMALL INTERFERING;

MAMMALIA;

EID: 33745227243     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2006.05.056     Document Type: Article

References (23)

1. Hammond S.M. Dicing and slicing: the core machinery of the RNA interference pathway. FEBS Lett. 579 (2005) 5822-5829
2. Elbashir S.M., Lendeckel W., and Tuschl T. RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev. 15 (2001) 188-200
3. Elbashir S.M., Martinez J., Patkaniowska A., Lendeckel W., and Tuschl T. Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate. EMBO J. 20 (2001) 6877-6888
4. Bartel D.P. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116 (2004) 281-297
5. Zeng Y., Yi R., and Cullen B.R. MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms. Proc. Natl. Acad. Sci. USA 100 (2003) 9779-9784
6. Hutvagner G., and Zamore P.D. A microRNA in a multiple-turnover RNAi enzyme complex. Science 297 (2002) 2056-2060
7. Doench J.G., Petersen C.P., and Sharp P.A. siRNAs can function as miRNAs. Genes Dev. 17 (2003) 438-442
8. Bagga S., Bracht J., Hunter S., Massirer K., Holtz J., Eachus R., and Pasquinelli A.E. Regulation by let-7 and lin-4 miRNAs results in target mRNA degradation. Cell 122 (2005) 553-563
9. Lim L.P., et al. Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Nature 433 (2005) 769-773
10. Yu Z., Raabe T., and Hecht N.B. MicroRNA Mirn122a reduces expression of the posttranscriptionally regulated germ cell transition protein 2 (Tnp2) messenger RNA (mRNA) by mRNA cleavage. Biol. Reprod. 73 (2005) 427-433
11. Yekta S., Shih I.H., and Bartel D.P. MicroRNA-directed cleavage of HOXB8 mRNA. Science 304 (2004) 594-596
12. Liu J., Valencia-Sanchez M.A., Hannon G.J., and Parker R. MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies. Nat. Cell Biol. 7 (2005) 719-723
13. Sen G.L., and Blau H.M. Argonaute 2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies. Nat. Cell Biol. 7 (2005) 633-636
14. Liu J., Rivas F.V., Wohlschlegel J., Yates III J.R., Parker R., and Hannon G.J. A role for the P-body component GW182 in microRNA function. Nat. Cell Biol. 7 (2005) 1161-1166
15. Pillai R.S., Bhattacharyya S.N., Artus C.G., Zoller T., Cougot N., Basyuk E., Bertrand E., and Filipowicz W. Inhibition of translational initiation by Let-7 MicroRNA in human cells. Science 309 (2005) 1573-1576
16. Rehwinkel J., Behm-Ansmant I., Gatfield D., and Izaurralde E. A crucial role for GW182 and the DCP1:DCP2 decapping complex in miRNA-mediated gene silencing. RNA 11 (2005) 1640-1647
17. Lewis B.P., Burge C.B., and Bartel D.P. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell 120 (2005) 15-20
18. Lee R.C., Feinbaum R.L., and Ambros V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 75 (1993) 843-854
19. Wightman B., Ha I., and Ruvkun G. Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans. Cell 75 (1993) 855-862
20. Germann U.A., Chambers T.C., Ambudkar S.V., Licht T., Cardarelli C.O., Pastan I., and Gottesman M.M. Characterization of phosphorylation-defective mutants of human P-glycoprotein expressed in mammalian cells. J. Biol. Chem. 271 (1996) 1708-1716
21. Roschke A.V., et al. Karyotypic complexity of the NCI-60 drug-screening panel. Cancer Res. 63 (2003) 8634-8647
22. Birmingham A., et al. 3′ UTR seed matches, but not overall identity, are associated with RNAi off-targets. Nat. Meth. 3 (2006) 199-204
23. Saxena S., Jonsson Z.O., and Dutta A. Small RNAs with imperfect match to endogenous mRNA repress translation. Implications for off-target activity of small inhibitory RNA in mammalian cells. J. Biol. Chem. 278 (2003) 44312-44319