The substrate specificity of cruzipain 2, a cysteine protease isoform from Trypanosoma cruzi

FEMS Microbiology Letters

Volumn 259, Issue 2, 2006, Pages 215-220

  Dos Reis, Flavia C G ^a   Júdice, Wagner A S ^b   Juliano, Maria A ^b   Juliano, Luiz ^b   Scharfstein, Julio ^a   De A Lima, Ana Paula C ^a  

^a FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^b FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Chagas Disease; Cysteine protease; Isoform; Specificity; Trypanosome

Indexed keywords

CRUZIPAIN; CRUZIPAIN 2; CYSTEINE PROTEINASE; ISOENZYME; UNCLASSIFIED DRUG;

ARTICLE; ENZYME SPECIFICITY; NONHUMAN; NONPHOTOCHEMICAL QUENCHING; PRIORITY JOURNAL; TRYPANOSOMA CRUZI;

MASTIGOPHORA (FLAGELLATES); TRYPANOSOMA CRUZI;

EID: 33744813530     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2006.00267.x     Document Type: Article

References (31)

1. Aparicio IM, Scharfstein J Lima APCA 2004 A new cruzipain-dependent pathway of human cell invasion by Trypanosoma cruzi requiring trypomastigote membranes. Infect Immun 72: 5892 5902.
2. Aslund L, Henriksson J, Campetella O, Frasch CC, Petterson U Cazzulo JJ 1990 The C-terminal extension of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi. Mol Biochem Parasitol 45: 345 348.
3. Brinen LS, Hansell E, Cheng J, Roush WR, McKerrow JH Fletterick RJ 2000 A target within the target: probing cruzain's P′1 site to define structural determinants for the Chagas' disease protease. Structure 8: 831 840.
4. Campetella O, Henriksson J, Aslund L, Frasch ACC, Pettterson U Cazzulo JJ 1992 The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is encoded by multiple polymorphic tandemly organized genes located on different chromosomes. Mol Biochem Parasitol 50: 225 234.
5. Cazzulo JJ, Cazzulo Franke MC, Martinez J Franke de Cazzulo BM 1990a Some kinetic properties of a cysteine proteinase (cruzipain) from Trypanosoma cruzi. Biochim Biophys Acta 1037: 186 191.
6. Cazzulo JJ, Cazzulo Franke MC, Martinez J Franke de Cazzulo BM 1990b Some kinetic properties of a cysteine proteinase (cruzipain) from Trypanosoma cruzi. Biochim Biophys Acta 1037: 186 191.
7. Cazzulo JJ, Bravo M, Raimondi A, Engstrom U, Lindeberg G Hellman U 1996 Hydrolysis of synthetic peptides by cruzipain, the major cysteine proteinase from Trypanosoma cruzi, provides evidence for self-processing and the possibility of more specific substrates for the enzyme. Cell Mol Biol 42: 691 696.
8. Cazzulo JJ, Stoka V Turk V 1997 Cruzipain, the major cysteine proteinase from the protozoan parasite Trypanosoma cruzi. Biol Chem 378: 1 10.
9. Eakin RE, Mills RR, Harth G, McKerrow JH Craik CS 1992 The sequence, organization, and expression of the major cysteine protease (Cruzain) from Trypanosoma cruzi. J Biol Chem 267: 7411 7420.
10. Engel JC, Doyle PS, Hsieh I McKerrow JH 1998 Cysteine protease inhibitors cure an experimental Trypanosoma cruzi infection. J Exp Med 188: 725 734.
11. Gillmor SR, Craik CS Fletterick RJ 1997 Structural determinants of specificity in the cysteine protease cruzain. Prot Sci 6: 1603 1611.
12. Harth G, Andrews N, Mills RR, Engel JC, Smith R McKerrow JH 1993 Peptide-fluoromethyl ketones arrest intracellular replication and intercellular transmission of T. cruzi. Mol Biochem Parasitol 58: 17 24.
13. Judice WA, Cezari MHS, Lima APCA, Scharfstein J, Chagas JR, Tersariol ILS, Juliano MA Juliano L 2001 Comparison of the specificity, stability and individual rate constants with respective activation parameters for the peptidase activity of cruzipain and its recombinant form, cruzain, from Trypanosoma cruzi. Eur J Biochem 268: 6578 6586.
14. Lima APCA, Scharfstein J, Storer AC Ménard R 1992 Temperature dependent substrate inhibition of the cysteine proteinase (GP57/51) from Trypanosoma cruzi. Mol Biochem Parasitol 56: 335 338.
15. Lima APCA, Tessier DC, Thomas DY, Scharfstein J, Storer AC Vernet T 1994 Identification of new cysteine protease isoforms in Trypanosoma cruzi. Mol Biochem Parasitol 67: 333 338.
16. Lima APCA, Reis FCG, Serveau C, Lalmanach G, Juliano L, Ménard R, Vernet T, Thomas DY, Storer AC Scharfstein J 2001 Cysteine protease isoforms from Trypanosoma cruzi, cruzipain 2 and cruzain, present different substrate preference and susceptibility to inhibitors. Mol Biochem Parasitol 114: 41 52.
17. McGrath ME, Eakin RE, Engel JC, Mckerrow JH, Craik CS Fletterick RA 1995 The crystal structure of cruzain: a therapeutic target for Chagas' Disease. J Mol Biol 247: 251 259.
18. Meirelles MNL, Juliano L, Carmona E, Silva SG, Costa EM, Murta AC Scharfstein J 1992 Inhibitors of the major cysteinyl proteinase (GP57/51) impair host cell invasion and arrest the intracellular development of Trypanosoma cruziin vitro. Mol Biochem Parasitol 52: 175 184.
19. Meldal M, Svendsen IB, Juliano L, Juliano MA, Nery ED Scharfstein J 1998 Inhibition of cruzipain visualized in a fluorescence quenched solid-phase inhibitor library assay. d-amino acid inhibitors for cruzipain, cathepsin B and cathepsin L. J Peptide Sci 4: 83 91.
20. Murta ACM, Persechini PM, Souto-Padron T, De Souza W, Guimarães JR Scharfstein J 1990 Structural and functional identification of GP57/51 antigen of Trypanosoma cruzi as a cysteine proteinase. Mol Biochem Parasitol 43: 27 38.
21. Nagler DK Ménard R 2003 Family C1 cysteine proteases: biological diversity or redundancy? Biol Chem 384: 837 843.
22. Nery ED, Juliano MA, Meldal M, Svendsen I, Scharfstein J, Walmsley A Juliano L 1997 Characterization of the substrate specificity of the major cysteine protease (cruzipain) from Trypanosoma cruzi using a portion-mixing combinatorial library and fluorogenic peptides. Biochem J 323: 427 433.
23. Parodi AJ, Labriola C Cazzulo JJ 1995 The presence of complex-type oligosaccharides at the C-terminal domain glycosylation site of some molecules of cruzipain. Mol Biochem Parasitol 69: 247 255.
24. Sajid M McKerrow JH 2002 Cysteine proteases of parasitic organisms. Mol Biochem Parasitol 120: 1 21.
25. Scharfstein J 2003 Activation of bradykinin-receptors by Trypanosoma cruzi: a role for cruzipain in microvascular pathology. Molecular Pathogenesis of Chagas' Disease Kelly J.M., ed), pp. 111 137. Landes Bioscience, Austin, TX.
26. Scharfstein J, Schmitz V, Morandi V, Capella MM, Lima APCA, Morrot A, Juliano L Muller-Esterl W 2000 Host cell invasion by Trypanosoma cruzi is potentiated by activation of bradykinin B2 receptors. J Exp Med 192: 1289 1330.
27. Serveau C, Lalmanach G, Juliano MA, Scharfstein J, Juliano L Gauthier F 1996 Investigation of the substrate specificity of cruzipain, the major cysteine proteinase of T. cruzi, through the use of cystatin-derived substrates and inhibitors. Biochem J 313: 951 1956.
28. Serveau C, Lalmanach G, Hirata I, Scharfstein J, Juliano MA Gauthier F 1999 Discrimination of cruzipain, the major cysteine proteinase of Trypanosoma cruzi, and mammalian cathepsins B and L, by a pH-inducible fluorogenic substrate of trypanosomal cysteine proteinases. Eur J Biochem 259: 275 280.
29. Tomas A Kelly JM 1996 Stage-regulated expression of cruzipain, the major cysteine protease of Trypanosoma cruzi is independent of the level of RNA. Mol Biochem Parasitol 76: 91 103.
30. Turk D, Guncar G, Podohnick M Turk B 1998 Revised definition of binding sites of papain-like cysteine proteases. Biol Chem 379: 137 147.
31. Wang B, Shi GP, Yao PM, Li Z, Chapman HA Bromme D 1998 Human cathepsin F, molecular cloning, functional expression, tissue localization, and enzymatic characterization. J Biol Chem 273: 32000 32008.