Structural determinants of specificity in the cysteine protease cruzain

Protein Science

Volumn 6, Issue 8, 1997, Pages 1603-1611

  Gillmor, Sarah A ^a   Craik, Charles S ^a   Fletterick, Robert J ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cysteine protease; Parasite diseases; pH switch; Specificity; Structure based drug design; Trypanosoma cruzi; X ray structure

Indexed keywords

CRUZIPAIN;

ARTICLE; CONTROLLED STUDY; ENZYME CONFORMATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; STRUCTURE ACTIVITY RELATION; TRYPANOSOMA CRUZI; X RAY ANALYSIS;

ESCHERICHIA COLI; TRYPANOSOMA; TRYPANOSOMA CRUZI;

EID: 0030841381     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560060801     Document Type: Article

References (30)

1. Barrett AJ, Kirschke H. 1981. Cathepsin B, cathepsin H, and cathepsin L. Methods Enzymol 80 C:535-561.
2. Berti PJ, Storer AC. 1995. Alignment/phylogeny of the papain superfamily of cysteine proteases. J Mol Biol 246:273-283.
3. Bone R, Fujishige A, Kettner CA, Agard DA. 1991. Structural basis for broad specificity in alpha-lytic protease mutants. Biochemistry 30:10388-10398.
4. Brömme D, Bonneau PR, Lanchance P, Storer AC. 1994. Engineering the S2 subsite specificity of human cathepsin S to a cathepsin L and cathepsin B-like specificity. J Biol Chem 269:30238-30242.
5. Brünger AT. 1993. Assessment of phase accuracy by cross validation - The free R-value - Methods and applications. Acta Crystallogr D 49:24-36.
6. Brünger AT, Kuriyan J, Karplus M. 1987. Crystallographic R-factor refinement by molecular dynamics. Science 235:458-460.
7. Craik CS, Largman C, Fletcher T, Roczniak S, Barr PJ, Fletterick R, Rutter WJ. 1985. Redesigning trypsin: Alteration of substrate specificity. Science 228:291-297.
8. Creighton TE. 1993. Proteins: Structures and molecular properties. New York: W.H. Feeman and Co.
9. Cygler M, Sivaraman J, Grochulski P, Coulombe R, Storer AC, Mort JS. 1996. Structure of rat procathepsin B: Model for inhibition of cysteine protease activity by the proregion. Structure 4:405-416.
10. Eakin AE, McGrath ME, McKerrow JH, Fletterick RJ, Craik CS. 1993. Production of crystallizable cruzain, the major cysteine protease from Trypanosoma cruzi. J Biol Chem 268:6115-6118.
11. Eakin AE, Mills AA, Harth G, McKerrow JH, Craik CS. 1992. The sequence, organization, and expression of the major cysteine protease (cruzain) from Trypanosoma cruzi. J Biol Chem 267:7411-7420.
12. Harth G, Andrews N, Mills AA, Engel J, Smith R, McKerrow JH. 1993. Peptide-fluoromethyl ketones arrest intracellular replication and intercellular transmission of T. cruzi. Mol Biochem Parasitol 58:17-24.
13. Hedstrom L, Szilagyi L, Rutter WJ. 1992. Converting trypsin to chymotrypsin: The role of surface loops. Science 255:1249-1253.
14. Hof P, Mayr I, Huber R, Korzus E, Potempa J, Travis J, Powers J C, Bode W. 1996. The 1.8 Å crystal structure of human cathepsin G in complex with Suc-Val-Pro-Phe-(Oph)2: A janus-faced proteinase with two opposite specificities. EMBO J 15:5481-5491.
15. Jia Z, Hasnain S, Hirama T, Lee X, Mort JS, To R, Huber CP. 1995. Crystal structures of recombinant rat cathepsin B and a cathepsin B-inhibitor complex. J Biol Chem 270:5527-5533.
16. Khouri HE, Plouffe C, Hasnain S, Hirama T, Storer AC, Ménard R. 1991. A model to explain the pH-dependent specificity of cathepsin B-catalyzed hydrolyses. Biochem J 275:751-757.
17. McGrath ME, Eakin AE, Engel JC, McKerrow JH, Craik CS, Fletterick RJ. 1995. The crystal structure of cruzain: A therapeutic target for Chagas' disease. J Mol Biol 247:251-259.
18. Muller K, Ammann HJ, Doran DM, Gerber PR, Gubernator K, Schrepfer G. 1988. Moloc: A molecular modelling program. Bull Soc Chim Belg 97:655-667.
19. Musil D, Zucic D, Turk D, Engh RA, Mayr I, Huber R, Popovic T, Turk V, Towatar T, Katunuma N, Bode W. 1991. The refined 2.15 Å X-ray crystal structure of human liver cathepsin B: The structural basis for its specificity. EMBO J 10:2321-2330.
20. Nascimento AE, de Souza W. 1996. High resolution localization of cruzipain and ssp4 in Trypanosoma cruzi by replica staining of label fracture. Biology of the Cell 86:53-58.
21. Perona JJ, Craik CS. 1995. Structural basis of substrate specificity in the serine proteases. Protein Sci 4:337-360.
22. Perona JJ, Tsu CA, Craik CS, Fletterick RJ. 1996. The crystal structure of an ecotin-collagenase complex suggests a model for recognition and cleavage of the collagen triple helix. Biochemistry. Forthcoming.
23. Ponder JW, Richards FM. 1987. Tertiary templates for proteins: Use of packing criteria in the enumeration of allowed sequences for different structural classes. J Mol Biol 193:775-791.
24. Rosenthal PJ, McKerrow JH, Aikawa M, Nagasawa H, Leech JH. 1988. A malarial cysteine proteinase is necessary for hemoglobin degradation by Plasmodium falciparum. J Clin Invest 82:1560-1566.
25. Sack JS. 1988. Chain - A crystallographic modelling program. J Mol Graphics 6:244-245.
26. Schecter I, Berger A. 1967. On the size of the active site in proteases. I. Papain. Biochem Biophys Res Commun 27:157-163.
27. Serveau C, Lalmanach G, Juliano MA, Scharfstein J, Juliano L, Gauthier F. 1995. Investigation of the substrate specificity of cruzipain, the major cysteine proteinase of Trypanosoma cruzi, through the use of cystalin-derived substrates and inhibitors. Biochem J 313:951-956.
28. Tsu CA, Perona JJ, Schellenberger V, Turck CW, Craik CS, 1994. The substrate specificity of Uca pugilator collagenolytic serine protease 1 correlates with the bovine type I collagen cleavage sites. J Biol Chem 269:19565-19572.
29. Turk D, Podobnick M, Popovic T, Katunuma N, Bode W, Huber R, Turk V. 1995. Crystal structure of cathepsin B inhibited with CA030 at 2.0 Å resolution: A basis for the design of specific epoxysuccinyl inhibitors. Biochemistry 34:4791-4797.
30. Wells JA, Powers DB, Bott RR, Graycar TP, Estell DA. 1987. Designing substrate specificity by protein engineering of electrostatic interactions. Proc Natl Acad Sci USA 84:1219-1223.