Comparison of the specificity, stability and individual rate constants with respective activation parameters for the peptidase activity of cruzipain and its recombinant form, cruzain, from trypanosoma cruzi

European Journal of Biochemistry

Volumn 268, Issue 24, 2001, Pages 6578-6586

  Judice, Wagner A S ^a   Cezari, Maria Helena S ^a   Lima, Ana Paula C A ^b   Scharfstein, Julio ^b   Chagas, Jair Ribeiro ^c   Tersariol, Ivarne L S ^c   Juliano, Maria A ^a   Juliano, Luiz ^a  

^a FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

^b FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^c UNIVERSIDADE DE MOGI DAS CRUZES   (Brazil)

Author keywords

C terminal domain; Cruzain; Cruzipain; Cysteine proteinase; Substrate specificity.

Indexed keywords

ASPARTIC ACID; CRUZIPAIN; CYSTEINE; MANNOSE; PEPTIDASE; PEPTIDE DERIVATIVE; PROTEINASE; RECOMBINANT ENZYME; SODIUM CHLORIDE;

ACYLATION; ARTICLE; CARBOXY TERMINAL SEQUENCE; COMPARATIVE STUDY; CONTROLLED STUDY; DEACYLATION; DIFFUSION; DISSOCIATION; ENERGY; ENTROPY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME DENATURATION; ENZYME GLYCOSYLATION; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SUBSTRATE; HYDROLYSIS; MICHAELIS CONSTANT; NONHUMAN; PH; PRIORITY JOURNAL; TEMPERATURE DEPENDENCE; TRYPANOSOMA CRUZI;

AMINO ACID SEQUENCE; ANIMALS; CYSTEINE ENDOPEPTIDASES; ENZYME ACTIVATION; ENZYME STABILITY; HEAT; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; OLIGOPEPTIDES; PROTEIN DENATURATION; PROTOZOAN PROTEINS; RECOMBINANT PROTEINS; SODIUM CHLORIDE; SUBSTRATE SPECIFICITY; TRYPANOSOMA CRUZI;

PROTOZOA; TRYPANOSOMA; TRYPANOSOMA CRUZI;

EID: 0035660408     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0014-2956.2001.02612.x     Document Type: Article

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